Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process

Mattos, Ives Bernardelli de; Alves, Dilson Amancio; Hollanda, Luciana Maria de; Ceragiogli, Helder J; Baranauskas, Vı́tor; Lancellotti, Marcelo

doi:10.1186/1477-3155-9-53

Cited by 14 publications

(8 citation statements)

References 41 publications

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“…It has to be noticed that several publications have already shown that nanoparticles may be involved in a better transformation efficiency 40 – 45 . But, in all theses cases, the authors demonstrated that it was due to a mechanical effect of the nanoparticles: protection of the DNA from DNase activity by interaction between DNA and nanoparticles 41 or damage to the cell envelope which improves the DNA uptake 43 – 45 , i.e. phenomena that occur only under laboratory growth conditions in an agitated liquid medium.…”

Section: Discussionmentioning

confidence: 99%

Impact of nanoparticles on the Bacillus subtilis (3610) competence

Eymard-Vernain

Luche

Rabilloud

et al. 2018

Sci Rep

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Due to the physicochemical properties of nanoparticles, the use of nanomaterials increases every year in industrial and medical processes. At the same time, the increasing number of bacteria becoming resistant to many antibiotics, mostly by a horizontal gene transfer process, is a major public health concern. We herein report, for the first time, the role of nanoparticles in the physiological induction of horizontal gene transfer in bacteria. Besides the most well-known impacts of nanoparticles on bacteria, i.e. death or oxidative stress, two nanoparticles, n-ZnO and n-TiO2, significantly and oppositely impact the transformation efficiency of Bacillus subtilis in biofilm growth conditions, by modification of the physiological processes involved in the induction of competence, the first step of transformation. This effect is the consequence of a physiological adaptation rather than a physical cell injury: two oligopeptide ABC transporters, OppABCDF and AppDFABC, are differentially expressed in response to nanoparticles. Interestingly, a third tested nanoparticle, n-Ag, has no significant effect on competence in our experimental conditions. Overall, these results show that nanoparticles, by altering bacterial physiology and especially competence, may have profound influences in unsuspected areas, such as the dissemination of antibiotic resistance in bacteria.

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Section: Discussionmentioning

confidence: 99%

Impact of nanoparticles on the Bacillus subtilis (3610) competence

Eymard-Vernain

Luche

Rabilloud

et al. 2018

Sci Rep

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“…Another recent report has also revealed that ZnO NP promoted competence, whereas TiO 2 decreased competence, in B. subtilis during biofilm mode of growth 45 . Several other studies have also shown that nanoparticles can augment transformation efficiency in bacteria 46–48 . But studies on the ability of NPs to cure plasmid in bacteria are scarce 37 .…”

Section: Discussionmentioning

confidence: 85%

Sub lethal levels of platinum nanoparticle cures plasmid and in combination with carbapenem, curtails carbapenem resistant Escherichia coli

Bharathan

Sundaramoorthy

Chandrasekaran

et al. 2019

Sci Rep

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Drug resistance traits are rapidly disseminated across bacteria by horizontal gene transfer, especially through plasmids. Plasmid curing agents that are active both in vitro and in vivo will resensitize Multi Drug Resistant (MDR) bacteria to antimicrobial agents. Pectin capped platinum nanoparticles (PtNPs) at sub MIC (20 µM) concentration was effective, in causing loss of Extended Spectrum Beta Lactamase (ESBL) harboring plasmid as evidenced by, absence of plasmid in agarose gel and by a concomitant (16–64 fold) drop in MIC for cell wall inhibitors ceftriaxone and meropenem, in carbapenem resistant Escherichia coli (CREC). Interestingly, the plasmid cured strain exhibited small colony morphology and displayed slower growth both in vitro and in vivo . Complementation of cured strain with plasmid from the wild type strain restored resistance towards meropenem and ceftriaxone. Relative to wild type, plasmid cured strain displayed 50% reduction in biofilm formation. Plasmid curing also occurred in vivo in infected zebrafish with curing efficiency of 17% for nanoparticle + meropenem treatment. PtNPs + meropenem reduced bioburden of CREC in infected zebrafish by 2.4 log CFU. Mechanistic studies revealed that nanoparticle interacted with cell surface and perturbed inner membrane integrity. PtNPs did not induce ROS, yet it caused plasmid DNA cleavage, as evidenced by gyrase inhibition assay. Our study for the first time reveals that PtNPs as plasmid curing agent can resensitize MDR bacteria to selective antimicrobial agents in vivo .

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“…The transformation experiments using the S. pneumoniae strain ATCC33400 was performed using as donor DNA the mutant for quorum sensing gene luxS. The transforming units obtained from the mix of the peptide, bacterium and synthetic carbon nanotubes (Mattos et al, 2011), and mesoporous silica SBa15 and SBa16. The tests were performed in triplicate and the results shown in the Figure 1.…”

Section: Resultsmentioning

confidence: 99%