Effects of Moxibustion on Stress-Induced Delayed Gastric Emptying via Somatoautonomic Reflex in Rats

Abstract

Objective: Moxibustion (MOX) is used to treat a wide variety of disorders, including those with gastric symptoms. However, the exact mechanisms underlying the beneficial effects of MOX are unknown. The purpose of this study was to investigate if application of indirect MOX (iMOX) to ST 36 reduces restraint stress (RS)-induced alteration in gastric responses of conscious rats, and if a somatoautonomic reflex mediates gastric emptying (GE). Materials and Methods: One group of rats was fed solid food after 24 hours of fasting. Immediately after food ingestion. These rats were subjected to RS. Ninety minutes after feeding, the rats were euthanized, and their gastric contents were removed to calculate GE. iMOX had been performed at ST 36 bilaterally throughout the stress loading. To investigate if vagal-nerve activity was involved in mediating the stress-induced alterations of GE by iMOX, atropine was intraperitoneally administered to other rats just before initiating RS; bilateral truncal vagotomy had been performed on day 14 before GE measurement. Results: RS delayed GE significantly (42.9 -5.8%)in stressed rats, compared to nonstressed rats (68.7 -1.8%). iMOX at ST 36 reduced stress-induced inhibition of GE significantly (67.1 -2.4%). MOX-mediated reduction of GE disappeared upon atropine injection and vagotomy. Conclusions: RS-induced delayed GE may be ameliorated by iMOX at ST 36. Somatoautonomic, reflexinduced vagal-nerve activity helps mediate the stimulatory effects of iMOX on RS-induced delayed GE. As a complementary and alternative medicine, iMOX may also be advantageous for patients with gastric disorders, such as functional dyspepsia.