Effects of morphology regulated by Pb2+ on graphene oxide cytotoxicity: Spectroscopic and in vitro investigations

Qiang, Shirong; Wang, Mingyuan; Liang, Jianjun; Zhao, Xuejiao; Fan, Qiaohui; Geng, Rongyue; Luo, Dongxia; Li, Zhengbin; Zhang, Li

doi:10.1016/j.matchemphys.2019.122016

Cited by 6 publications

(6 citation statements)

References 36 publications

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“…Again, an interesting result was that the H 2 O 2 level in the CeO 2 -RGO nanocomposites was significantly lower than the RGO group and was not statistically different from the control group ( Figure 8C). In similarity, Qiang et al [26] observed that Pb 2+ reduced the cytotoxicity, oxidative stress, and apoptotic response of GO through the regulation of the GO morphology. The equilibrium between pro-oxidant generation and their scavenging by antioxidant molecules and enzymes is a delicate phenomenon.…”

Section: Oxidative Stressmentioning

confidence: 88%

“…The process of apoptosis can be triggered by several factors, including nutrient deficiency, growth factors, and environmental contaminants [41]. GO and RGO have shown potential to induce apoptosis in human cells [7,18,19,26]. Ali et al [18] also reported that Ag-doped RGO triggered apoptosis in human hepatic normal and carcinoma cells.…”

Section: Apoptosismentioning

confidence: 99%

“…Moreover, the widespread applications of these nanocomposites may increase their chance of exposure to human lung cells. In this regard, A549 cells have been widely used by toxicology and pharmacology researchers [26]. CeO 2 -RGO nanocomposites were prepared by a simple hydrothermal method.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Cytotoxicity and Oxidative Stress Response of CeO2-RGO Nanocomposites in Human Lung Epithelial A549 Cells

et al. 2019

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Graphene-based nanocomposites have attracted enormous interest in nanomedicine and environmental remediation, owing to their unique characteristics. The increased production and widespread application of these nanocomposites might raise concern about their adverse health effects. In this study, for the first time, we examine the cytotoxicity and oxidative stress response of a relatively new nanocomposite of cerium oxide-reduced graphene oxide (CeO2-RGO) in human lung epithelial (A549) cells. CeO2-RGO nanocomposites and RGO were prepared by a simple hydrothermal method and characterized by relevant analytical techniques. Cytotoxicity data have shown that RGO significantly induces toxicity in A549 cells, evident by cell viability reduction, membrane damage, cell cycle arrest, and mitochondrial membrane potential loss. However, CeO2-RGO nanocomposites did not cause statistically significant toxicity as compared to a control. We further observed that RGO significantly induces reactive oxygen species generation and reduces glutathione levels. However, CeO2-RGO nanocomposites did not induce oxidative stress in A549 cells. Interestingly, we observed that CeO2 nanoparticles (NPs) alone significantly increase glutathione (GSH) levels in A549 cells as compared to a control. The GSH replenishing potential of CeO2 nanoparticles could be one of the possible reasons for the biocompatible nature of CeO2-RGO nanocomposites. Our data warrant further and more advanced research to explore the biocompatibility/safety mechanisms of CeO2-RGO nanocomposites in different cell lines and animal models.

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Section: Oxidative Stressmentioning

confidence: 88%

Section: Apoptosismentioning

confidence: 99%

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Evaluation of the Cytotoxicity and Oxidative Stress Response of CeO2-RGO Nanocomposites in Human Lung Epithelial A549 Cells

et al. 2019

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“…Due to its excellent physical, chemical, and mechanical properties, graphene and its derivatives have been extensively studied and have been used to prepare new composite materials [1][2][3][4]. Particularly, graphene oxide (GO), a graphene derivative, possesses both hydrophilic and hydrophobic oxygen-containing functional groups, such as hydroxyl, carboxyl, epoxy groups, thereby making graphene oxide soluble in water and a number of organic solvents [5]. Owing to its high solubility, GO has been widely applied in various biomedical applications, such as biological sensing, heat-based tumor targeting, drug delivery, and tissue engineering [4,6].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli

Qiang

Zhang

et al. 2021

Nanomaterials

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The biological and environmental toxicity of graphene and graphene derivatives have attracted great research interest due to their increasing applications. However, the cytotoxic mechanism is poorly understood. Here, we investigated the cytotoxic effect of graphene oxide nanoribbons (GORs) on Escherichia coli (E. coli) in an in vitro method. The fabricated GORs formed long ribbons, 200 nm wide. Based on the results of the MTT assay and plate-culture experiments, GORs significantly inhibited the growth and reproduction of E. coli in a concentration-dependent manner. We found that GORs stimulated E. coli to secrete reactive oxygen species, which then oxidized and damaged the bacterial cell membrane. Moreover, interaction between GORs and E. coli cytomembrane resulted in polysaccharide adsorption by GORs and the release of lactic dehydrogenase. Furthermore, GORs effectively depleted the metal ions as nutrients in the culture medium by adsorption. Notably, mechanical cutting by GORs was not obvious, which is quite different from the case of graphene oxide sheets to E. coli.

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“…Apoptosis is regulated by various factors such as environmental contaminants, growth factors and deficiency of nutrients [42]. Apoptotic response to GO and RGO is also reported in recent literature [43,44]. Ali et al [45] observed that Ag-doped RGO induce apoptosis in human liver cells.…”

Section: Apoptotic Response Of Rgo and Fe3o4-rgo Nanocompositesmentioning

confidence: 87%

Investigation of Cytotoxicity, Apoptosis, and Oxidative Stress Response of Fe3O4-RGO Nanocomposites in Human Liver HepG2 cells

2020

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Iron oxide–reduced graphene oxide (Fe3O4-RGO) nanocomposites have attracted enormous interest in the biomedical field. However, studies on biological response of Fe3O4-RGO nanocomposites at the cellular and molecular level are scarce. This study was designed to synthesize, characterize, and explore the cytotoxicity of Fe3O4-RGO nanocomposites in human liver (HepG2) cells. Potential mechanisms of cytotoxicity of Fe3O4-RGO nanocomposites were further explored through oxidative stress. Prepared samples were characterized by UV-visible spectrophotometer, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and energy dispersive spectroscopy. The results demonstrated that RGO induce dose-dependent cytotoxicity in HepG2 cells. However, Fe3O4-RGO nanocomposites were not toxic. We further noted that RGO induce apoptosis in HepG2 cells, as evidenced by mitochondrial membrane potential loss, higher caspase-3 enzyme activity, and cell cycle arrest. On the other hand, Fe3O4-RGO nanocomposites did not alter these apoptotic parameters. Moreover, we observed that RGO increases intracellular reactive oxygen species and hydrogen peroxide while decrease antioxidant glutathione. Again, Fe3O4-RGO nanocomposites did not exert oxidative stress. Altogether, we found that RGO significantly induced cytotoxicity, apoptosis and oxidative stress. However, Fe3O4-RGO nanocomposites showed good biocompatibility to HepG2 cells. This study warrants further research to investigate the biological response of Fe3O4-RGO nanocomposites at the gene and molecular level.

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Effects of morphology regulated by Pb2+ on graphene oxide cytotoxicity: Spectroscopic and in vitro investigations

Cited by 6 publications

References 36 publications

Evaluation of the Cytotoxicity and Oxidative Stress Response of CeO2-RGO Nanocomposites in Human Lung Epithelial A549 Cells

Evaluation of the Cytotoxicity and Oxidative Stress Response of CeO2-RGO Nanocomposites in Human Lung Epithelial A549 Cells

Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli

Investigation of Cytotoxicity, Apoptosis, and Oxidative Stress Response of Fe3O4-RGO Nanocomposites in Human Liver HepG2 cells

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