Effects of Morphogen and Scaffold Porogen on the Differentiation of Dental Pulp Stem Cells

Demarco, Flávio Fernando; Casagrande, Luciano; Zhang, Zhaocheng; Dong, Zhihong; Tarquínio, Sandra Beatriz Chaves; Zeitlin, Benjamin David; Shi, Songtao; Smith, Anthony J.; Nör, Jacques E.

doi:10.1016/j.joen.2010.08.031

Cited by 115 publications

(112 citation statements)

References 36 publications

(43 reference statements)

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“…Scaffold's pore size has been shown to affect the cell attachment, proliferation, migration, morphology and gene expression (10,13). The smaller the scaffold's pore size the greater the specific surface area for cell attachment on its surface; however, the cell migration can be hindered (12).…”

Section: Introductionmentioning

confidence: 99%

Influence of Poly-L-Lactic Acid Scaffold's Pore Size on the Proliferation and Differentiation of Dental Pulp Stem Cells

et al. 2015

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The aim of this study was to evaluate the influence of the poly-L-lactic acid (PLLA)-based scaffold's pore size on the proliferation and differentiation of dental pulp stem cells (DPSCs). The scaffolds were prepared in pulp chambers of 1-mm-thick tooth slices from third molars using salt crystals (150-250 µm or 251-450 µm) as porogen. DPSC (1x10 5 cells) were seeded in the scaffolds with different pore sizes, and cultured in 24-well plates. The cell proliferation was evaluated using the WST-1 assay after 3-21 days. Furthermore, RT-PCR was used to assess the differentiation of the DPSCs into odontoblasts, using markers of odontoblastic differentiation (DSPP, DSP-1 and MEPE). RNA from human odontoblasts was used as control. Cell proliferation rate was similar in both scaffolds except at the 14 th day period, in which the cells seeded in the scaffolds with larger pores showed higher proliferation (p<0.05). After 21 days DPSCs seeded in both evaluated scaffolds were able of expressing odontoblastic markers DMP-1, DSPP and MEPE. In summary, both scaffolds tested in this study allowed the proliferation and differentiation of DPSCs into odontoblast-like cells.

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Section: Introductionmentioning

confidence: 99%

Influence of Poly-L-Lactic Acid Scaffold's Pore Size on the Proliferation and Differentiation of Dental Pulp Stem Cells

et al. 2015

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“…Both cell-based and cell-homing approaches showed various aspects necessary for pulp regeneration (14)(15)(16). Some of these studies already focused on the use of irrigating solutions (10,17) and the type of scaffold (18)(19)(20). In the current study, the fibrin gel showed distinctive tissue in-growth without cell transplantation.…”

Section: Discussionmentioning

confidence: 64%

Fibrin Gel Improves Tissue Ingrowth and Cell Differentiation in Human Immature Premolars Implanted in Rats

Ruangsawasdi

Zehnder

Weber

2014

Journal of Endodontics

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INTRODUCTION In pulpless immature human premolars implanted in rodents, this study investigated whether fibrin gel offered advantages over leaving the root canal empty regarding soft tissue ingrowth and cell differentiation. METHODS Root canals of extracted human immature premolars (n = 12) were accessed and then irrigated with 5% sodium hypochlorite followed by 17% ethylenediaminetetraacetic acid. Root canals were then either left empty or filled with a fibrin gel (n = 6 each) before being placed subcutaneously on top of the calvarial bone of rats (1 tooth per rat) for 12 weeks. After sacrifice, teeth were histologically assessed. Tissue ingrowth was quantified and compared between groups using the Mann-Whitney U test (P < .05). Cells adhering to the pulp canal wall were immunohistochemically screened for the presence of bone sialoprotein (BSP) and dentin sialoprotein (DSP). RESULTS More tissue grew into the pulp space when teeth were filled with fibrin gel (P < .05). The presence of fibrin gel affected not only the extent of tissue ingrowth but also tissue morphology and differentiation of cells contacting the dentinal wall. In the fibrin gel group, newly formed tissue was similar to normal pulp, constituted of inner pulp, cell-rich zone, cell-free zone, and an apparent odontoblast layer, which stained positive for BSP and DSP. Newly formed blood vessels were also more abundant compared with the initially empty root canals. CONCLUSIONS Under the conditions of this study, fibrin gel improved cell infiltration and cell-dentin interaction. Both are necessary for pulp tissue regeneration.

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“…Several in vivo study models have been developed and conducted using many cell lines 1,2,36,38,40 . Synthetic and natural scaffolds have been developed and applied successfully 16,24,26 . Moreover, several techniques used in modern clinical dentistry emerged from the application of tissue engineering principles.…”

Section: Discussionmentioning

confidence: 99%

“…The spacing between the pores formed and the scaffold stiffness and topography will be important in determining the type of tissue formed 26 . Any change in this microenvironment is capable of altering cellular behavior.…”

Section: Scaffolds In Regenerative Therapiesmentioning

confidence: 99%

“…TS/S is an in vivo model where horizontal sections in the region of the cement/enamel junction are performed, so the tissue that is present is removed. The presence of the surrounding dentin is one of the key points of the model, since its prior conditioning with ethylenediaminetetraacetic acid (EDTA) releases the growth factors trapped in this tissue 26 . In addition, the tooth slice obtained is filled by PLLA scaffolds and these are seeded with stem cells.…”

Section: Scaffolds In Regenerative Therapiesmentioning

confidence: 99%

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What is the clinical applicability of regenerative therapies in dentistry?

Demarco

Kirschnick

Watson

et al. 2017

RGO, Rev. Gaúch. Odontol.

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Regenerative therapies have been widely developed in dentistry and it is important to incorporate dentists' knowledge of these new therapies RESUMOTerapias regenerativas vem sendo amplamente desenvolvidas na odontologia e o conhecimento destas novas terapias por parte dos dentistas é importante para que elas sejam incorporadas na rotina clínica odontológica. Assim, este estudo revisou a literatura acerca das terapias regenerativas e suas aplicações clínicas. A engenharia tecidual tem contribuído na mudança do paradigma restaurador das ciências da saúde. Seus pilares embasam as técnicas de regeneração de tecidos e órgãos. Apesar da grande maioria dos estudos neste campo ser in vitro, uma gama de metodologias pré-clínicas foi consolidada e estudos utilizando estes princípios já estão sendo empregados em humanos. A utilização de plasma rico em plaquetas e plasma rico em fibrina como scaffolds naturais em cirurgias para reestabelecimento de tecido ósseo e periodontal são frequentemente relatadas na literatura e ensaios clínicos utilizando esta abordagem demonstram resultados promissores. Células-tronco da polpa dental autólogas já foram aplicadas com sucesso na regeneração de tecido ósseo utilizando scaffold naturais de colágeno em humanos. Além disto, a revascularização do canal radicular já aparece na literatura como uma alternativa promissora frente a opção de apecificação do canal radicular. Esta terapia utiliza como princípio o coágulo sanguíneo como scaffold e a migração das células-tronco da papila apical para regenerar o órgão pulpar. Considerações finais: Apesar de incipientes, as terapias regenerativas já podem ser utilizadas na prática clínica odontológica. O conhecimento dos princípios que regem estas terapias deve ser compreendido pelo dentista para que sejam utilizadas na prática clínica.Termos de indexação: Fibrina. Plasma rico em plaquetas. Células-tronco. Engenharia tecidual.

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Effects of Morphogen and Scaffold Porogen on the Differentiation of Dental Pulp Stem Cells

Cited by 115 publications

References 36 publications

Influence of Poly-L-Lactic Acid Scaffold's Pore Size on the Proliferation and Differentiation of Dental Pulp Stem Cells

Influence of Poly-L-Lactic Acid Scaffold's Pore Size on the Proliferation and Differentiation of Dental Pulp Stem Cells

Fibrin Gel Improves Tissue Ingrowth and Cell Differentiation in Human Immature Premolars Implanted in Rats

What is the clinical applicability of regenerative therapies in dentistry?

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