Effects of Moringa oleifera Seed Oil on Cultured Human Sebocytes In Vitro and Comparison with Other Oil Types

Abstract

The seeds of Moringa oleifera (horseradish tree) contain about 40% of one of the most stable vegetable oils (Moringa seed oil). Therefore, the effects of Moringa seed oil on human SZ95 sebocytes were investigated and were compared with other vegetable oils. Immortalized human SZ95 sebocytes were treated with Moringa seed oil, olive oil, sunflower oil, linoleic acid and oleic acid. Lipid droplets were visualized by Nile Red fluorescence, cytokine secretion via cytokine antibody array, cell viability with calcein-AM fluorescence, cell proliferation by real-time cell analysis, and fatty acids were determined by gas chromatography. Statistical analysis was performed by the Wilcoxon matched-pairs signed-rank test, the Kruskal–Wallis test and Dunn’s multiple comparison test. The vegetable oils tested stimulated sebaceous lipogenesis in a concentration-dependent manner. The pattern of lipogenesis induced by Moringa seed oil and olive oil was comparable to lipogenesis stimulated by oleic acid with also similar fatty acid secretion and cell proliferation patterns. Sunflower oil induced the strongest lipogenesis among the tested oils and fatty acids. There were also differences in cytokine secretion, induced by treatment with different oils. Moringa seed oil and olive oil, but not sunflower oil, reduced the secretion of pro-inflammatory cytokines, in comparison to untreated cells, and exhibited a low n-6/n-3 index. The anti-inflammatory oleic acid detected in Moringa seed oil probably contributed to its low levels of pro-inflammatory cytokine secretion and induction of cell death. In conclusion, Moringa seed oil seems to concentrate several desired oil properties on sebocytes, such as high content level of the anti-inflammatory fatty acid oleic acid, induction of similar cell proliferation and lipogenesis patterns compared with oleic acid, lipogenesis with a low n-6/n-3 index and inhibition of secretion of pro-inflammatory cytokines. These properties characterize Moringa seed oil as an interesting nutrient and a promising ingredient in skin care products.

The Effect of Adding Different Proportions of Local Moringa oleifera Seed Oil on the Shelf Life Extension of Flaxseed Oil

“…Moringa oleifera extracts are one of the most important natural alternatives to chemical antioxidants, which pose a health risk due to their cumulative effect [6]. Moringa oleifera seed oil is one of the most important natural alternatives as it contains tocopherol, phenols, carotenoids and sterols [7][8][9]. In IOP Publishing doi:10.1088/1755-1315/1371/6/062026 2 addition to the high content of oleic acid, this is important for extending the shelf life of oils and increasing their stability [8,10].…”

“…Moringa oleifera seed oil is one of the most important natural alternatives as it contains tocopherol, phenols, carotenoids and sterols [7][8][9]. In IOP Publishing doi:10.1088/1755-1315/1371/6/062026 2 addition to the high content of oleic acid, this is important for extending the shelf life of oils and increasing their stability [8,10]. Flaxseed oil is distinguished by its high content of unsaturated fatty acids, which reaches 85%.…”

Linoleic Acid Induced Changes in SZ95 Sebocytes—Comparison with Palmitic Acid and Arachidonic Acid

“…Among the sebum components, LA is central. It contributes to the structural organisation and function of the permeability barrier of the stratum corneum [9] and also stimulates lipid production, increasing cell size and promoting differentiation and the delayed proliferation of sebocytes [10][11][12]. In pathological conditions such as acne-one of the most common inflammatory skin diseases associated with SGs-reduced levels of LA compared with other species have been associated with increased microcomedone formation and increased follicular cast size in affected patients.…”