Effects of Moringa oleifera lam. Leaf Powder on Bifidobacteria and Escherichia coli in the Gut of Albino Rats

Abstract

Aim: This study was carried out to determine the effects of dried Moringa oleifera leaves on Bifidobacteria and Escherichia coli in the gut of albino rats.
 Location: The rats were habituated under laboratory conditions at the animal house of the Department of Zoology, Faculty of Science, University of Ibadan, for two weeks in other to adapt to the environmental conditions during the experiment.
 Duration of Study: The rats were exposed to the M. oleifera feed for four weeks.
 Design of Study: There were five groups in all. The 5 to 6 weeks old rats were fed with M. oleifera powder supplement except for the control groups.
 Methods: No supplement of M. oleifera feed was administered to group A while group B received streptomycin antibiotics.
 Groups C, D and E received dried leaf supplement of M. oleifera (DMO) 1.25 g/kg body weight (2.5%), 2.5 g/kg body weight (5%) and 5.0 g/kg body weight (10%) respectively.
 Results: E.coli counts increased from 2.3*104 to 2.6*104 colony-forming units per gram (cfu/g) in group E, from 2.2*104 to 3.0*10 cfu/g in group B; but reduced from 4.1*104 to 3.7*104 cfu/g in group D and from 5.4*104 to 3.9*104 cfu/g in group C between day 20 and day 28. As from day 8, the isolates from the non-control groups were resistant to the M. oleifera extract except E. coli isolates in both 5% and 10% M. oleifera groups on day 8 with 6 mm zone of inhibition each. The rate of Bifidobacteria viable counts increase in group E was expressed as P = 0.05 at the beginning of the experiment, unlike E.coli counts where there was a decrease.
 Conclusion: The M. oleifera leaf alters the microbiota in the gut, a situation which sends impulses to the brain. Thus, the M. oleifera leaf powder is a potential prebiotic for probiotics like Bifidobacteria, and as well as induce changes in the gut-brain axis.

Effect of paprika extracts on growth performance, haemolymph chemistry, intestinal microbiota and antioxidant enzyme activities of white‐leg shrimp (Litopenaeus vannamei)

“…However, the intestinal microbiota and its stability depend on intrinsic factors of the host, such as age, gender and genotype, as well as extrinsic factors such as habitat and diet (Ringø et al, 2016; Xu & Knight, 2015). Therefore, diet additive including probiotic or herbal supplements, affects the intestinal microbiota and may lead to a balance in the gut microbial population by improving the growth of beneficial bacteria (Abidin et al, 2022; Amoah et al, 2020; Gbadebo et al, 2019; Habiba et al, 2021; Munaeni et al, 2020). In this study, the total number of heterotrophic bacteria in the intestine decreased significantly with an increase in the concentration of paprika extract.…”

Deciphering the influence of dietary synbiotics in white shrimp gut and its effects in regulating immune signaling pathways

“…Aquatic microbiologists were more interested in studying the gut microbiota because it plays a significant role in the growth of its hosts through a variety of mechanisms, such as producing digestive enzymes for food digestion (Amin, 2018), excreting vitamins , and producing short-chain fatty acids (SCFAs) (Hoseinifar et al, 2017). Dietary management, such as probiotic and prebiotics supplementation, affects the gut microbiota composition (Gbadebo et al, 2019;Amoah et al, 2020;Munaeni et al, 2020). The growth performance of shrimp fed with diets containing a combination of a probiotic and a prebiotic is higher than that of shrimp fed with diets containing a probiotic or prebiotic alone (Abidin et al, 2022) Moreover, many researchers also found an increase in growth performance by using multiple probiotics (Lee et al, 2022).…”

Effect of Moringa Oleifera Supplementation during Pregnancy on the Prevention of Stunted Growth in Children between the Ages of 36 to 42 Months

“…For example, flavonoids are found to have a large anti-oxidant function and increase urination and electrolyte excretion, which functions like potassium that absorbs electrolyte ions. 27 In addition, the phenolic chemicals in moringa are multi-functional compounds that are useful for pregnant women and children in preventing cell damage, diseases and aging. 28 Previous research also shows that giving EG to pregnant women increases the quantity of their breast milk.…”