Effects of Moringa Extract on Aminoglycoside-Induced Hair Cell Death and Organ of Corti Damage

Broderick, Michael; Prince, Andrew D P; Dhukhwa, Asmita; Mukherjea, Debashree; Jiang, Peng; Campbell, Kathleen C.M.; Rybak, Leonard P.; Brenner, Michael

doi:10.1097/mao.0000000000003193

Cited by 2 publications

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liu et al reported that CA significantly ameliorated BV-2 microglial cell damage caused by lipopolysaccharide, improved mitochondria functions, and reduced MAPK/NF-κB-mediated mechanical stress. Administration of water extract from Moringa Oleifera protected against aminoglycoside-induced hair cells loss and reversed mitochondrial membrane potential of Corti explants through suppressing ROS production [ 39 ]. Protective actions exerted by stimulating Keap1/Nrf2 transcription activity resulted in an antioxidant defense capacity that ultimately restored the intracellular redox status in a C57BL/6J mice model [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Cichoric Acid May Play a Role in Protecting Hair Cells from Ototoxic Drugs

Lai

Cheng

et al. 2022

IJMS

View full text Add to dashboard Cite

Ototoxic hearing loss due to antibiotic medication including aminoglycosides and excess free radical production causes irreversible hair cell injury. Cichoric acid, a naturally occurring phenolic acid, has recently been found to exert anti-oxidative and anti-inflammatory properties through its free radical scavenging capacity. The present study aimed to investigate the protective effects of cichoric acid against neomycin-induced ototoxicity using transgenic zebrafish (pvalb3b: TagGFP). Our results indicated that cichoric acid in concentrations up to 5 μM did not affect zebrafish viability during the 2 h treatment period. Therefore, the otoprotective concentration of cichoric acid was identified as 5 μM under 2 h treatment by counting viable hair cells within the neuromasts of the anterior- and posterior-lateral lines in the study. Pretreatment of transgenic zebrafish with 5 μM of cichoric acid for 2 h significantly protected against neomycin-induced hair cell death. Protection mediated by cichoric acid was, however, lost over time. A terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and FM4-64 staining, respectively, provided in situ evidence that cichoric acid ameliorated apoptotic signals and mechanotransduction machinery impairment caused by neomycin. A fish locomotor test (distance move, velocity, and rotation frequency) assessing behavioral alteration after ototoxic damage revealed rescue due to cichoric acid pretreatment before neomycin exposure. These findings suggest that cichoric acid in 5 μM under 2 h treatment has antioxidant effects and can attenuate neomycin-induced hair cell death in neuromasts. Although cichoric acid offered otoprotection, there is only a small difference between pharmacological and toxic concentrations, and hence cichoric acid can be considered a rather prototypical compound for the development of safer otoprotective compounds.

show abstract

Section: Discussionmentioning

confidence: 99%

Cichoric Acid May Play a Role in Protecting Hair Cells from Ototoxic Drugs

Lai

Cheng

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

Aminoglycosides-Related Ototoxicity: Mechanisms, Risk Factors, and Prevention in Pediatric Patients

Rivetti,

Romano,

Mastrangelo

et al. 2023

Pharmaceuticals

View full text Add to dashboard Cite

Aminoglycosides are broad-spectrum antibiotics largely used in children, but they have potential toxic side effects, including ototoxicity. Ototoxicity from aminoglycosides is permanent and is a consequence of its action on the inner ear cells via multiple mechanisms. Both uncontrollable risk factors and controllable risk factors are involved in the pathogenesis of aminoglycoside-related ototoxicity and, because of the irreversibility of ototoxicity, an important undertaking for preventing ototoxicity includes antibiotic stewardship to limit the use of aminoglycosides. Aminoglycosides are fundamental in the treatment of numerous infectious conditions at neonatal and pediatric age. In childhood, normal auditory function ensures adequate neurocognitive and social development. Hearing damage from aminoglycosides can therefore strongly affect the normal growth of the child. This review describes the molecular mechanisms of aminoglycoside-related ototoxicity and analyzes the risk factors and the potential otoprotective strategies in pediatric patients.

show abstract

Effects of Moringa Extract on Aminoglycoside-Induced Hair Cell Death and Organ of Corti Damage

Cited by 2 publications

References 43 publications

Cichoric Acid May Play a Role in Protecting Hair Cells from Ototoxic Drugs

Cichoric Acid May Play a Role in Protecting Hair Cells from Ototoxic Drugs

Aminoglycosides-Related Ototoxicity: Mechanisms, Risk Factors, and Prevention in Pediatric Patients

Contact Info

Product

Resources

About