Effects of Mogrosides on High-Fat-Diet-Induced Obesity and Nonalcoholic Fatty Liver Disease in Mice

Zhang, Xiaobing; Song, Yunfei; Ding, Yipei; Wang, Wei; Liao, Ling; Zhong, Jin; Sun, Pengbo; Fan, Lei; Zhang, Yaou; Xie, Weidong

doi:10.3390/molecules23081894

Cited by 32 publications

(20 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, we observed that the oocyte activation rate was reduced, and the development of competence was enhanced after oocytes were exposed to MV during in vitro ageing. As MV is an antioxidant that can directly scavenge free radicals [36] and inhibit ROS production in the cultured cells [18, 20] and the IVM porcine oocytes [37], our results demonstrated that MV reduced ROS levels in aged oocytes. As expected, MV ameliorated the abnormal microtubule assembly and chromosomal misalignment in oocytes caused by in vitro ageing.…”

Section: Discussionmentioning

confidence: 69%

“…In addition, several studies have reported that MV is a powerful antioxidant that scavenges free radicals [15]. Mogrosides, the primary component of which is MV, reduce oxidative stress in the livers of high-fat diet-induced obese mice and alloxan-induced diabetic mice [16–18]. In addition, MV reduces the production of pro-inflammatory cytokines, protecting lung tissue against acute lung injury induced by lipopolysaccharide (LPS) [19].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation

Nie

Sui

Zhang

et al. 2019

Aging

View full text Add to dashboard Cite

Postovulatory ageing compromises oocyte quality and subsequent development in various manners. We aimed to assay the protective effects of mogroside V on porcine oocyte quality during in vitro ageing and explore the related causes. We observed that mogroside V can effectively maintain normal oocyte morphology and early embryo development competence after prolonged culture for 24 h. Moreover, mogroside V can markedly reduce reactive oxygen species (ROS) levels, alleviate spindle formation and chromosome alignment abnormalities, improve mitochondrial contents, adenosine triphosphate (ATP) levels and the membrane potential (ΔΨm), and reduce early apoptosis in aged oocytes. We examined the molecular changes and found that SIRT1 expression was decreased in in vitro aged oocytes but was maintained by exposure to mogroside V. However, when SIRT1 was successfully inhibited by the specific inhibitor EX-527, mogroside V could not reduce ROS levels or alleviate abnormal spindle organization and chromosome misalignment. In summary, our results demonstrated that mogroside V can alleviate the deterioration of oocyte quality during in vitro ageing, possibly by reducing oxidative stress through SIRT1 upregulation.

show abstract

Section: Discussionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation

Nie

Sui

Zhang

et al. 2019

Aging

View full text Add to dashboard Cite

show abstract

“… 7 , 11 Due to their sweet taste and nontoxic characteristic, they are used as beverages for patients who present with obesity and diabetes. 12 Recently, their bioactivities including anti-bacterial, anti-allergic, anti-inflammatory and anti-diabetic effects have been reported. 13 Mogroside IIIE (MG IIIE), the most widely distributed metabolite, can reduce blood glucose, and alleviate the clinical symptoms of pancreas at the early stage and pathological lesions.…”

Section: Introductionmentioning

confidence: 99%

<p>Mogroside IIIE Alleviates High Glucose-Induced Inflammation, Oxidative Stress and Apoptosis of Podocytes by the Activation of AMPK/SIRT1 Signaling Pathway</p>

Xue¹,

Mao²,

Chen³

et al. 2020

DMSO

View full text Add to dashboard Cite

Background: Diabetic nephropathy (DN) is the leading cause of impaired renal function. The purpose of this study was to investigate the effects of Mogroside IIIE (MG IIIE), a cucurbitane-type compound isolated from Siraitia grosvenorii, in high glucose (HG)induced podocytes and the possible mechanisms. Methods: MPC-5 cells were cultured under normal glucose or HG conditions. After treatment with MG IIIE, cell viability was examined using a cell counting kit-8 assay. The contents of inflammatory factors and oxidative stress-related markers were determined using the corresponding kits. Additionally, apoptosis of MPC-5 cells was determined using flow cytometry assay and the levels of apoptosis-associated proteins were evaluated by Western blot analysis. Moreover, the expression of proteins in AMPK/SIRT1 signaling was tested and the compound C, an AMPK inhibitor, was used to study whether the effects of MG IIIE on HG-induced MPC-5 cells were mediated by activation of the AMPK/SIRT1 signaling pathway. Results: MG IIIE elevated the cell viability of HG-induced MPC-5 cells, reduced the concentrations of inflammatory cytokines and decreased the levels of oxidative stressrelated markers. What's more, the apoptosis of podocytes induced by HG was inhibited after MG IIIE intervention, accompanied by the upregulated expression of Bcl-2 and downregulated expression of Bax, cleaved caspase-3 and cleaved caspase-9. It was also found that MG IIIE could activate the AMPK/SIRT1 signaling, but compound C inhibited this pathway and reversed the inhibitory effects of MG IIIE on inflammation, oxidative stress and apoptosis in HG-stimulated podocytes. Conclusion: MG IIIE can alleviate HG-induced inflammation and oxidative stress of podocytes by the activation of AMPK-SIRT1 signaling.

show abstract

“…Currently, animal models for obesity research mainly are pigs, mice, and rats [3][4][5]. The pig is emerging rapidly as a biomedical model of energy metabolism and obesity in humans because it is postnatally devoid of brown fat and because of similar metabolic features, cardiovascular systems, and proportional organ sizes [6].…”

Section: Introductionmentioning

confidence: 99%

Relationship among porcine lncRNA TCONS_00010987, miR-323, and leptin receptor based on dual luciferase reporter gene assays and expression patterns

Ding

Wang

et al. 2020

Asian-Australas J Anim Sci

View full text Add to dashboard Cite

Considering the physiological and clinical importance of leptin receptor (LEPR) in regulating obesity and the fact that porcine LEPR expression is not known to be controlled by lncRNAs and miRNAs, we aim to characterize this gene as a potential target of SSC-miR-323 and the lncRNA TCONS_00010987. Methods: Bioinformatics analyses revealed that lncRNA TCONS_00010987 and LEPR have SSC-miR-323-binding sites and that LEPR might be a target of lncRNA TCONS_00010987 based on cis prediction. Wild-type and mutant TCONS_00010987-target sequence fragments and wild-type and mutant LEPR 3′-UTR fragments were generated and cloned into pmiR-RB-REPORT TM-Control vectors to construct respective recombinant plasmids. HEK293T cells were co-transfected with the SSC-miR-323 mimics or a negative control with constructs harboring the corresponding binding sites and relative luciferase activities were determined. Tissue expression patterns of lncRNA TCONS_00010987, SSC-miR-323, and LEPR in Anqing six-end-white (AQ, the obese breed) and Large White (LW, the lean breed) pigs were detected by real-time quantitative polymerase chain reaction; backfat expression of LEPR protein was detected by western blotting. Results: Target gene fragments were successfully cloned, and the four recombinant vectors were constructed. Compared to the negative control, SSC-miR-323 mimics significantly inhibited luciferase activity from the wild-type TCONS_00010987-target sequence and wildtype LEPR-3′-UTR (p<0.01 for both) but not from the mutant TCONS_00010987-target sequence and mutant LEPR-3′-UTR (p>0.05 for both). Backfat expression levels of TCONS_ 00010987 and LEPR in AQ pigs were significantly higher than those in LW pigs (p<0.01), whereas levels of SSC-miR-323 in AQ pigs were significantly lower than those in LW pigs (p<0.05). LEPR protein levels in the backfat tissues of AQ pigs were markedly higher than those in LW pigs (p<0.01). Conclusion: LEPR is a potential target of SSC-miR-323, and TCONS_00010987 might act as a sponge for SSC-miR-323 to regulate LEPR expression.

show abstract

Effects of Mogrosides on High-Fat-Diet-Induced Obesity and Nonalcoholic Fatty Liver Disease in Mice

Cited by 32 publications

References 24 publications

Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation

Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation

<p>Mogroside IIIE Alleviates High Glucose-Induced Inflammation, Oxidative Stress and Apoptosis of Podocytes by the Activation of AMPK/SIRT1 Signaling Pathway</p>

Relationship among porcine lncRNA TCONS_00010987, miR-323, and leptin receptor based on dual luciferase reporter gene assays and expression patterns

Contact Info

Product

Resources

About