Effects of Modified Sanzi Yangqin Decoction on Tyrosine Phosphorylation of IRS-1 in Skeletal Muscle of Type 2 Diabetic Rats

Wang, Ya; La, Xiaojin; Tian, Chunyu; Dong, Yan; Feng, Qi; Chang-long, Qiu; Gao, Xiujuan; Li, Jian

doi:10.1155/2018/7092140

Cited by 3 publications

(5 citation statements)

References 21 publications

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“…In present study, diabetic model induced by HFD and low-dose STZ displays the raise in fasting blood glucose, glycated hemoglobin, and ratio of heart weight to body weight, as well as decreased fasting serum insulin level and ventricular function. These results confirm the successfully established diabetic model with cardiomyopathy that is consistent with previous studies [ 16 , 31 ].…”

Section: Discussionsupporting

confidence: 93%

Cardioprotective effects of polydatin against myocardial injury in diabetic rats via inhibition of NADPH oxidase and NF-κB activities

Tan

Chen

Fang

et al. 2020

BMC Complement Med Ther

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Background Diabetic cardiomyopathy is a main cause of the increased morbidity in diabetic patients, no effective treatment is available so far. Polydatin, a resveratrol glucoside isolated from the Polygonum cuspidatum, was found by our and others have antioxidant and cardioprotective activities. Therapeutic effects of polydatin on diabetic cardiomyopathy and the possible mechanisms remains unclear. This study aimed to investigate the cardioprotective effects and underlying mechanisms of polydatin on myocardial injury induced by hyperglycemia. Methods Diabetes in rats was made by high-fat diet combined with multiple low doses of streptozotocin, and then treated with polydatin (100 mg·kg-1·day-1, by gavage) for 8 weeks. Cardiac function was examined by echocardiography. Myocardial tissue and blood samples were collected for histology, protein and metabolic characteristics analysis. In cultured H9c2 cells with 30 mM of glucose, the direct effects of polydatin on myocyte injury were also observed. Results In diabetic rats, polydatin administration significantly improved myocardial dysfunction and attenuated histological abnormalities, as evidenced by elevating left ventricular shortening fraction and ejection fraction, as well as reducing cardiac hypertrophy and interstitial fibrosis. In cultured H9c2 cells, pretreatment of polydatin dose-dependently inhibited high glucose-induced cardiomyocyte injury. Further observation evidenced that polydatin suppressed the increase in the reactive oxygen species levels, NADPH oxidase activity and inflammatory cytokines production induced by hyperglycemia in vivo and in vitro. Polydatin also prevented the increase expression of NOX4, NOX2 and NF-κB in the high glucose -stimulated H9c2 cells and diabetic hearts. Conclusions Our results demonstrate that the cardioprotective effect of polydatin against hyperglycemia-induced myocardial injury is mediated by inhibition of NADPH oxidase and NF-κB activity. The findings may provide a novel understanding the mechanisms of the polydatin to be a potential treatment of diabetic cardiomyopathy.

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Section: Discussionsupporting

confidence: 93%

Cardioprotective effects of polydatin against myocardial injury in diabetic rats via inhibition of NADPH oxidase and NF-κB activities

Tan

Chen

Fang

et al. 2020

BMC Complement Med Ther

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“…It has been reported that modified San Zi Yang Qin Decoction (SZ plus dodder and glossy privet) could reduce the levels of FBG and 2 h postprandial blood glucose and increase the glucose tolerance in type 2 diabetes model rats induced by high-fat diet and multiple low-dose streptozotocin injections [ 15 ], which is consistent with our results. In addition, natural compounds in SZ, such as luteolin, β -sitosterol, and stigmasterol, can counter NAFLD or NASH in rodents.…”

Section: Discussionsupporting

confidence: 93%

“…As one downstream molecule of IRS-1, AKT can be activated by phosphorylated IRS-1. Similarly, modified SZ can increase tyrosine-phosphorylated insulin receptor substrate-1 (IRS-1) in the skeletal muscle of T2DM rats to relieve insulin resistance [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…SZ is particularly effective for pulmonary diseases, such as intractable cough, chronic obstructive pneumonia disease (COPD), and chronic bronchitis [ 8 , 9 ]. According to the TCM theory of “treatment based on syndrome differentiation,” and “homotherapy for heteropathy” during which the same therapy is administered for different diseases sharing the same pathogenesis, SZ has been recently applied in the treatment of diseases resulting from the stagnation of phlegm, dampness, and Qi, like NAFLD, hyperlipidemia, type 2 diabetes mellitus (T2DM), and breast hyperplasia [ 10 – 15 ]. However, in vitro and in vivo experiments and evidence-based analyses have not been carried out to validate the efficacy and interpret the related biological mechanisms of SZ [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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A Study on the Therapeutic Efficacy of San Zi Yang Qin Decoction for Non-Alcoholic Fatty Liver Disease and the Underlying Mechanism Based on Network Pharmacology

Liu

Yang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Objective. This study aims to explore the therapeutic efficacy of San Zi Yang Qin Decoction (SZ) and its potential mechanism in the treatment of non-alcoholic fatty liver disease (NAFLD) based on network pharmacology and in vivo experiments. Methods. Effective chemicals and targets of SZ were searched in online databases, according to the drug-likeness of compounds and the binomial distribution of targets. A disease-target-chemical network was established using NAFLD-associated genes screened through GeneCards database, Gene Ontology (GO) terms, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Furthermore, animal experiments were conducted to verify the efficacy and mechanism of SZ predicted by network pharmacology. The NAFLD mouse model was established with C57BL/6J mice fed with a high-fat diet for 22 weeks. The mice in the control group were fed with a chow diet. From the 23rd week, the NAFLD mice were treated with intragastric SZ or normal saline for 8 weeks. After the glucose tolerance was measured, the mice were sacrificed, followed by the collection of serum and liver tissues. Pathological changes in liver tissues were examined by H&E staining. Additionally, alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum fast blood glucose, and insulin levels were detected. Expression levels of TNF-α of serum and liver tissues were determined by ELISA and qRT-PCR, respectively. Western blot was used to detect the activation of AKT in liver tissues. Results. A total of 27 effective compounds and 20 targets of SZ were screened. GO analysis uncovered a significant correlation between the targets of SZ and those of NAFLD. KEGG analysis presented the signaling pathways enriched in SZ and NAFLD, including NAFLD, TNF-α, and apoptosis pathways. The area under the curve of major GO and KEGG pathways indicated the potential role of SZ in improving NAFLD. In vivo experiments demonstrated that SZ significantly alleviated hepatosteatosis and inflammatory cell infiltration in liver tissues, reduced serum transaminases, and improved insulin resistance and glucose tolerance of NAFLD mice. The protein level of phospho-AKT was upregulated by SZ. Additionally, SZ treatment obviously impaired the TNF-α level in the serum and liver tissue of NAFLD mice. Conclusions. According to the network pharmacology analysis and in vivo experiments, SZ could have therapeutic efficacy for NALFD. The mechanism mainly involves pathways relative to insulin resistance, TNF-α, and apoptosis. Our results provide a scientific basis for SZ in the clinical treatment of NAFLD.

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“…Diabetes was confirmed six days after STZ injection using Glucocard™ 01-mini (Arkray Factory, Inc., Japan). Fasting blood glucose (FBG) of ≥11.1 mmol/L was considered in diabetic rats and was selected for the study ( Wang et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

The role of lutein-rich purple sweet potato leaf extract on the amelioration of diabetic retinopathy in streptozotocin-induced Sprague–Dawley rats

et al. 2023

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The objective of this study is to access the effect of purple sweet potato leaf (PSPL) extract on diabetic retinopathy (DR) of streptozotocin (STZ)-induced male Sprague–Dawley (SD) rats. In this study, rats were injected intraperitoneally with a single dose of 60 mg/kg STZ, and diabetes was confirmed on day 7. Rats were further divided into a few groups, which were then orally administered with one of the following treatments: 25 mg/kg of gliclazide (D25G), 200 mg/kg of PSPL extract (DT 200), and 400 mg/kg of PSPL extract (DT 400). However, the normal control (NS) and control group for diabetic (DNS) were given normal saline (NS) for 12 weeks. The results show that the treated group demonstrated a reduction in serum oral glucose tolerance test (OGTT) levels of DT 200 and DT 400, and an increase in the serum and retinal insulin levels, and restored oxidative stress markers in serum and retina on week 12. The PSPL extract exhibited protective effects in maintaining the kidney, liver, retina, and pancreas architecture in 400 mg/kg compared to the 200 mg/kg treated group and D25G, thereby restoring fully transparent lenses in diabetes-induced rats. In conclusion, 400 mg/kg PSPL is the most effective dose for the amelioration of STZ-induced DR pathology in male SD rats.

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Effects of Modified Sanzi Yangqin Decoction on Tyrosine Phosphorylation of IRS-1 in Skeletal Muscle of Type 2 Diabetic Rats

Cited by 3 publications

References 21 publications

Cardioprotective effects of polydatin against myocardial injury in diabetic rats via inhibition of NADPH oxidase and NF-κB activities

Cardioprotective effects of polydatin against myocardial injury in diabetic rats via inhibition of NADPH oxidase and NF-κB activities

A Study on the Therapeutic Efficacy of San Zi Yang Qin Decoction for Non-Alcoholic Fatty Liver Disease and the Underlying Mechanism Based on Network Pharmacology

The role of lutein-rich purple sweet potato leaf extract on the amelioration of diabetic retinopathy in streptozotocin-induced Sprague–Dawley rats

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