Effects of modified pellicles on <i>Candida</i> biofilm formation on acrylic surfaces

Nikawa, Hiroki; Nishimura, Haruki; Hamada, Takashi; Yamashiro, H.; Samaranayake, LP

doi:10.1046/j.1439-0507.1999.00270.x

Cited by 24 publications

(16 citation statements)

References 25 publications

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“…The presence of serum or salivary pellicles, which are normally found in the oral environment and may provide binding sites for Candida (8,10), increased the initial adherence of C. dubliniensis cells to biomaterials and subsequent biofilm formation. Other investigators have previously shown that the presence of serum and salivary pellicles can potentiate C. albicans colonization of acrylic strips and denture lining materials (41)(42)(43)(44)(45)(46). SEM techniques revealed that similar to C. albicans biofilms (5, 23), mature C. dubliniensis biofilms consist of a mixture of yeast and filamentous forms embedded within exopolymeric material.…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation by Candida dubliniensis

Ramage¹,

Walle

Wickes³

et al. 2001

J Clin Microbiol

167

156

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Candida dubliniensis is an opportunistic yeast closely related to Candida albicans that has been recently implicated in oropharyngeal candidiasis in human immunodeficiency virus-infected patients. Most manifestations of candidiasis are associated with biofilm formation, with cells in biofilms displaying properties dramatically different from free-living cells grown under normal laboratory conditions. Here, we report on the development of in vitro models of C. dubliniensis biofilms on the surfaces of biomaterials (polystyrene and acrylic) and on the characteristics associated with biofilm formation by this newly described species. Time course analysis using a formazan salt reduction assay to monitor metabolic activities of cells within the biofilm, together with microscopy studies, revealed that biofilm formation by C. dubliniensis occurred after initial focal adherence, followed by growth, proliferation, and maturation over 24 to 48 h. Serum and saliva preconditioning films enhanced the initial attachment of C. dubliniensis and subsequent biofilm formation. Scanning electron microscopy and confocal scanning laser microscopy were used to further characterize C. dubliniensis biofilms. Mature C. dubliniensis biofilms consisted of a dense network of yeasts cells and hyphal elements embedded within exopolymeric material. C. dubliniensis biofilms displayed spatial heterogeneity and an architecture showing microcolonies with ramifying water channels. Antifungal susceptibility testing demonstrated the increased resistance of sessile C. dubliniensis cells, including the type strain and eight different clinical isolates, against fluconazole and amphotericin B compared to their planktonic counterparts. C. dubliniensis biofilm formation may allow this species to maintain its ecological niche as a commensal and during infection with important clinical repercussions.

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Section: Discussionmentioning

confidence: 99%

Biofilm Formation by Candida dubliniensis

Ramage¹,

Walle

Wickes³

et al. 2001

J Clin Microbiol

167

156

View full text Add to dashboard Cite

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“…The ecologic niche for denture stomatitis, the oral cavity, is similar between rats and humans in terms of salivary flow, host immune response, and host salivary proteins. The role of saliva in Candida adhesion is complex (18,41,42). Saliva has a Compared to other commonly used laboratory animals, we found several advantages to employing a rat for the denture stomatitis model.…”

Section: Discussionmentioning

confidence: 99%

“…For example, cells in the in vitro systems are not exposed to salivary immune components and proteins which may precondition, or coat, the surface and promote adherence. Although several of the host components which play a key role in promoting Candida adherence have been defined, the interaction is likely a complex phenomenon (42). In the denture biofilm niche, additional components hypothesized to impact this growth state include companion oral bacterial flora, contact between the device biofilm and the host mucosal surface, and salivary flow dynamics.…”

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confidence: 99%

Development and Validation of an In Vivo Candida albicans Biofilm Denture Model

Marchillo²,

et al. 2010

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The most common form of oral candidiasis, denture-associated stomatitis, involves biofilm growth on an oral prosthetic surface. Cells in this unique environment are equipped to withstand host defenses and survive antifungal therapy. Studies of the biofilm process on dentures have primarily been limited to in vitro models. We developed a rodent acrylic denture model and characterized the Candida albicans and mixed oral bacterial flora biofilm formation, architecture, and drug resistance in vivo, using time course quantitative culture experiments, confocal microscopy, scanning electron microscopy, and antifungal susceptibility assays. We also examined the utility of the model for measurement of C. albicans gene expression and tested the impact of a specific gene product (Bcr1p) on biofilm formation. Finally, we assessed the mucosal host response to the denture biofilm and found the mucosal histopathology to be consistent with that of acute human denture stomatitis, demonstrating fungal invasion and neutrophil infiltration. This current oral denture model mimics human denture stomatitis and should be useful for testing the impact of gene disruption on biofilm formation, studying the impact of anti-infectives, examining the biology of mixed Candida-oral bacterial flora biofilm infections, and characterizing the host immunologic response to this disease process.

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“…Therefore, it has the capacity to reduce biofilm formation and inhibit the synthesis of insoluble polysaccharides in the microbial matrix of the dental biofilm 3,13,14 . This biochemical behavior may be explained by its excellent antimicrobial activity and its clinical effectiveness Antifungal activity of components used for decontamination… 141…”

Section: Discussionmentioning

confidence: 99%

“…for acrylic resin, to which it will adhere and colonize 3,4 . Effective oral and prosthesis hygiene is therefore essential for the maintenance of tissue viability and prevention of denture associated infections 5,6 .…”

mentioning

confidence: 99%

Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

Gouveia

Freire

Leite

et al. 2014

Rev. odontol. UNESP

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ResumoIntrodução: A efetividade de soluções antimicrobianas empregadas na descontaminação de próteses ainda é incerta. Objetivo: Avaliar a atividade antifúngica de soluções empregadas na descontaminação de próteses sobre o crescimento de Candida albicans. Material e método: Foram avaliados os produtos: Corega Tabs Branqueador  (S1), Hipoclorito de Sódio 1% (S2), Bicarbonato de Sódio 1% (S3), Peróxido de Hidrogênio 1% (S4), Digluconato de Clorexidina 0,12% -Periogard  (S5), Enxaguatório bucal a base de óleos essenciais -Listerine  (S6), e óleo essencial de Rosmarinus officinalis (alecrim) nas concentrações 1% (S7) e 2% (S8). A atividade antifúngica foi avaliada por meio da técnica de difusão em ágar e da determinação da curva de morte microbiana de amostras de C. albicans (ATCC 90028) na concentração 1,5×10 6 UFC/mL. Os testes foram realizados em triplicata e a análise estatística se deu pelos testes ANOVA Two-Way e Tukey, sendo adotado nível de confiança de 95%. Resultado: A média dos halos de inibição do crescimento, em milímetros, obtidos para os produtos foram: 0,0 (S1); 44,7 (S2); 0,0 (S3); 21,6 (S4); 10,0 (S5); 6,1 (S6); 0,0 (S7) e 2,4 (S8). Para curva de morte microbiana, todos os produtos apresentaram diferença estatisticamente significante (p<0,05) do grupo controle (cloreto de sódio 0,85%) e do grupo S3. Verificou-se crescimento fúngico inferior a 2×10 4 UFC/mL e acentuação na curva de morte microbiana após 30 minutos de ação, a exceção do grupo S3. Conclusão: As substâncias analisadas, a exceção do Bicarbonato de Sódio, possuem ação antifúngica frente C. albicans, podendo contribuir para higienização de próteses.Descritores: Produtos com ação antimicrobiana; Candida albicans; descontaminação. AbstractIntroduction: The effectiveness of antimicrobial solutions employed in dental prosthesis decontamination is still uncertain. Aim: To evaluate the antifungal activity of cleaners used in the decontamination of dental prostheses on the growth of Candida albicans. Material and method: The evaluated products were: Corega Tabs  (S1), Sodium Hypochlorite 1% (S2), Sodium Bicarbonate 1% (S3), Hydrogen Peroxide 1% (S4), Chlorhexidine Digluconate 0.12% -Periogard  (S5), Mouthrinse based on essential oils -Listerine  (S6), essential oil from Rosmarinus officinalis (rosemary) at concentrations of 1% (S7) and 2% (S8). The antifungal activity of the products was evaluated by agar diffusion technique and the determination of microbial death curve of samples of C. albicans (ATCC 90028) in concentration 1.5 × 10 6 CFU/mL. The tests were performed in triplicate and statistical analysis was made by ANOVA Two-Way and Tukey tests, with the confidence level of 95%. Result: The average of the zones of inhibition growth, in millimeters, obtained for the products were: 0.0 (S1), 44.7 (S2), 0.0 (S3), 21.6 (S4), 10.0 (S5), 6.1 (S6), 0.0 (S7) and 2.4 (S8). Considering the determination of microbial death curve, all products showed a statistical difference (p<0.01) from control (0.85% sodium chloride) and S3 groups. Fungal growt...

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Effects of modified pellicles on Candida biofilm formation on acrylic surfaces

Cited by 24 publications

References 25 publications

Biofilm Formation by Candida dubliniensis

Biofilm Formation by Candida dubliniensis

Development and Validation of an In Vivo Candida albicans Biofilm Denture Model

Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

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