“…Among these, the proportions of disordered structures were 51.2%, 48.6%, 51.1%, 51.6%, and 52.4%, whereas the proportions of ordered structures were 48.8%, 51.4%, 48.9%, 48.4%, and 47.6% for the AD group, HAD group, and HVCD groups with air-gap spacings of 7 cm, 8.5 cm, and 10 cm, respectively. During HVCD treatment, protein molecules undergo structural changes due to high-energy particle impact, resulting in not only alterations in their ordered structure but also an increase in the proportion of disordered structures [58][59][60]. The disorder of the alfalfa gradually increased as the air-gap spacing decreased.…”