Effects of Mobile Buffers on Facilitation: Experimental and Computational Studies

Tang, Yun-gui; Schlumpberger, Thomas; Kim, Taesung; Lueker, M.; Zucker, Robert S.

doi:10.1016/s0006-3495(00)76819-6

Cited by 85 publications

(105 citation statements)

References 62 publications

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“…Although these simulation results are coherent and in agreement with 2D simulations [36][37][38][39][40][41][44][45][46][47] , there are experimental 3D studies 31,35 that show a reverse result for the limiting diffusion coefficient and the anomalous diffusion exponent values.…”

Section: I) Effect Of Obstacle Density and Sizesupporting

confidence: 80%

New insights into diffusion in 3D crowded media by Monte Carlo simulations: effect of size, mobility and spatial distribution of obstacles

Vilaseca

Isvoran

Madurga

et al. 2011

Phys. Chem. Chem. Phys.

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Abstract. Particle diffusion in crowded media was studied through Monte Carlo simulations in 3D obstructed lattices. Three particular aspects affecting the diffusion, not extensively treated in three-dimensional geometry, were analysed: the relative particle-obstacle size, the relative particle-obstacle mobility and the way of having the obstacles distributed in the simulation space (randomly or uniformly). The results are interpreted in terms of the parameters that characterize the time dependence of the diffusion coefficient: the anomalous diffusion exponent (a), the crossover time from anomalous to normal diffusion regimes (τ) and the long time diffusion coefficient (D*). Simulation results indicate that there is a more anomalous diffusion (smaller a) and lower long time diffusion coefficient (D*) when obstacle concentration increases, and that, for a given total excluded volume and immobile obstacles, the anomalous diffusion effect is less important for bigger size obstacles. However, for the case of mobile obstacles, this size effect is inverted yielding values that are in qualitatively good agreement with in vitro experiments of protein diffusion in crowded media.These results underline that the pattern of the spatial partitioning of the obstacleexcluded volume is a factor to be considered together with the value of the excluded volume itself.

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Section: I) Effect Of Obstacle Density and Sizesupporting

confidence: 80%

New insights into diffusion in 3D crowded media by Monte Carlo simulations: effect of size, mobility and spatial distribution of obstacles

Vilaseca

Isvoran

Madurga

et al. 2011

Phys. Chem. Chem. Phys.

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“…3). Thus, facilitation in the predepressed state of the synapse is largely independent of Ca 2ϩ current facilitation and is probably mediated by a fast-equilibrating, high-affinity Ca 2ϩ binding site (Atluri and Regehr, 1996;Tang et al, 2000) and/or by Ca 2ϩ buffer saturation (Blatow et al, 2003;Felmy et al, 2003) (Müller et al, 2008, their Discussion). The rapid decay kinetics of this facilitation mechanism (ϳ30 ms), which is caused by the fast decay of residual free [Ca 2ϩ ] i in the nerve terminal (Müller et al, 2007), implies that short-term facilitation will only build up significantly when the stimulation frequency exceeds a value of ϳ1/ or ϳ30 Hz.…”

Section: Mechanism Of Facilitation In the Depressed State Of The Synapsementioning

confidence: 93%

Interaction between Facilitation and Depression at a Large CNS Synapse Reveals Mechanisms of Short-Term Plasticity

Müller

Goutman²,

Kochubey³

et al. 2010

J. Neurosci.

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The two fundamental forms of short-term plasticity, short-term depression and facilitation, coexist at most synapses, but little is known about their interaction. Here, we studied the interplay between short-term depression and facilitation at calyx of Held synapses. Stimulation at a "low" frequency of 10 or 20 Hz, which is in the range of the spontaneous activity of these auditory neurons in vivo, induced synaptic depression. Surprisingly, an instantaneous increase of the stimulation frequency to 100 or 200 Hz following the low-frequency train uncovered a robust facilitation of EPSCs relative to the predepressed amplitude level. This facilitation decayed rapidly (ϳ30 ms) and depended on presynaptic residual Ca 2ϩ , but it was not caused by Ca 2ϩ current facilitation. To probe the release probability of the remaining readily releasable vesicles following the low-frequency train we made presynaptic Ca 2ϩ uncaging experiments in the predepressed state of the synapse. We found that low-frequency stimulation depletes the fast-releasable vesicle pool (FRP) down to ϳ40% of control and that the remaining FRP vesicles are released with ϳ2-fold slower release kinetics, indicating a hitherto unknown intrinsic heterogeneity among FRP vesicles. Thus, vesicles with an intrinsically lower release probability predominate after low frequency stimulation and undergo facilitation during the onset of subsequent high-frequency trains. Facilitation in the predepressed state of the synapse might help to stabilize the amount of transmitter release at the onset of high-frequency firing at these auditory synapses.

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“…A great deal of information about motion of molecules in living cells has been obtained from intracellular measurements using different experimental techniques [7,[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] and from simulations [10,[31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49]. Experimental data are usually obtained by fluorescence recovery after photobleaching (FRAP) [12-14, 16, 18, 20, 28, 30], fluorescence correlation spectroscopy (FCS) [11-23, 26-27, 29] and single particle tracking (SPT) [15,19,[24][25] techniques.…”

Section: Introductionmentioning

confidence: 99%

Diffusion in macromolecular crowded media: Monte Carlo simulation of obstructed diffusion vs. FRAP experiments

et al. 2010

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Effects of Mobile Buffers on Facilitation: Experimental and Computational Studies

Cited by 85 publications

References 62 publications

New insights into diffusion in 3D crowded media by Monte Carlo simulations: effect of size, mobility and spatial distribution of obstacles

New insights into diffusion in 3D crowded media by Monte Carlo simulations: effect of size, mobility and spatial distribution of obstacles

Interaction between Facilitation and Depression at a Large CNS Synapse Reveals Mechanisms of Short-Term Plasticity

Diffusion in macromolecular crowded media: Monte Carlo simulation of obstructed diffusion vs. FRAP experiments

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