Effects of mixed Eimeria challenge on performance, body composition, intestinal health, and expression of nutrient transporter genes of Hy-Line W-36 pullets (0-6 wks of age)

Sharma, Milan Kumar; Liu, Guanchen; White, Dima; Tompkins, Yuguo Hou; Kim, Woo Kyun

doi:10.1016/j.psj.2022.102083

Cited by 25 publications

(61 citation statements)

References 36 publications

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“…During the early stages of egg production, a higher mineral content in the Cb may contribute to enhanced eggshell formation. Tb serves as a crucial indicator of bone health in mammals, with degradation of Tb microarchitecture, manifested by reduced thickness and volume along with increased spacing, being a hallmark feature of osteoporosis onset [42,45]. Akk exhibited the most pronounced effects on Tb.…”

Section: Discussionmentioning

confidence: 99%

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Akkermansia muciniphila Promotes Bone Development and Improves Eggshell Quality during the Sexual Maturity Period of Laying Hens by Increasing Osteogenesis

Lyu,

Zhang,

Yuan

et al. 2024

Agriculture

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Adequate bone development is imperative for maintaining the health and productivity of laying hens. Probiotics play a pivotal role in promoting bone formation and preventing osteoporosis. This study aimed to explore the effect of Akkermansia muciniphila (Akk) on the bone development and eggshell quality of laying hens during the rearing period. A total of 300 1-day-old Jingfen NO. 6 commercial pullets were categorized into two groups, one of which was fed a conventional diet for 20 weeks (Control group), the other group was fed a conventional diet with lyophilized Akk powder for 20 weeks (Akk group). During the first two weeks, pullets in the Akk group received live Akk inoculation, while birds in the Control group received normal saline administration. Micro-computed tomography analysis was employed to evaluate three bone microarchitectures: cortical bone (Cb), trabecular bone (Tb), and medullary bone (Mb). Our findings revealed that supplementation with Akk powder increased the thickness and bone mineral content of Cb and Tb, while simultaneously reducing the volume and bone surface area of Mb. The increased activity of alkaline phosphatase, a marker of osteogenesis, and the decreased activity of tartrate-resistant acid phosphatase, a marker of osteoclastic activity, were observed in the Akk group. Dietary supplementation of Akk powder improved the immune microenvironment in the bone marrow by increasing osteogenic-related CD8+ T cells and decreasing osteoclastogenesis-related CD4+ T cells. Additionally, Akk powder supplementation significantly enriched the Lactobacillaceae family in cecum. The enhancement of bone development by Akk contributed to increased eggshell strength and thickness. These findings demonstrate the osteomodulatory effects of Akk in laying hens and the connections between bone physiology and eggshell quality, highlighting the importance of gut–bone communications in laying hens.

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Section: Discussionmentioning

confidence: 99%

“…Image segmentation employed an adaptive-iterative threshold approach to differentiate between cortical bone (Cb), trabecular bone (Tb), and medullary bone (Mb). The different variables were measured and are described in Table 2 as defined by the previous study [42].…”

Section: Micro-computed Tomography Analysismentioning

confidence: 99%

Akkermansia muciniphila Promotes Bone Development and Improves Eggshell Quality during the Sexual Maturity Period of Laying Hens by Increasing Osteogenesis

Lyu,

Zhang,

Yuan

et al. 2024

Agriculture

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“…Birds were euthanized 2 h after the gavage, and blood was collected by cardiac puncture immediately after euthanasia. The blood was centrifuged at 1,000 × g for 15 min (Eppendorf Centrifuge 5430R, Eppendorf, Hamburg, Germany), and 100 μL of serum was used to determine the FITC-d concentration following the method described previously ( Sharma et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Coccidiosis, caused by the protozoan parasite of the genus Eimeria, has been a persistent challenge to the poultry industry, causing substantial financial losses worldwide annually ( Blake et al, 2020 ). The impact of coccidiosis in chickens can be severe, with damaged intestinal integrity, increased inflammation, and disturbed immune responses causing a decline in growth and, in some cases, even high mortality ( Castro et al, 2020a ; Teng et al, 2020 ; Sharma et al, 2022 ). Anticoccidial drugs have long been used to prevent coccidiosis ( Mesa-Pineda et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The effects of arginine and branched-chain amino acid supplementation to reduced-protein diet on intestinal health, cecal short-chain fatty acid profiles, and immune response in broiler chickens challenged with Eimeria spp.

et al. 2023

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“…Previous work has shed some light on the obnoxious effects of coccidiosis on the immune response of infected birds. For example, Sharma et al [ 12 ] reported increased production of pro-inflammatory cytokines and permeability of gastrointestinal tracts in 16-day old pullets inoculated with Eimeria spp. Separately, Pham et al [ 13 ] also reported that White Leghorn chickens, challenged with E. tenella , experienced diarrheal episodes and high intestinal lesion scores (e.g., loss of cecal villi, necrosis and hemorrhage), most likely due to the disruption of intestinal barrier functions in the animals, including downregulation of claudin (CLDN)-1, CLDN-3, E-cadherin, occludin ( OCLN ) and Zonula occludens-1 ( ZO-1 ), as well as increased levels of fluorescein isothiocyanate (FITC)-dextran, which indicated high intestinal permeability.…”

Section: Introductionmentioning

confidence: 99%

Dietary Weizmannia coagulans Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut Microbiota

et al. 2023

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To determine the mechanisms by which Weizmannia coagulans SANK70258 (WC) supplementation improved growth performance and coccidial symptoms, we assessed the gene expressions and the microbiota compositions in the small intestinal tissues and digestas of coccidium-infected broilers previously given WC or lasalocid-A sodium (AM). WC supplementation significantly upregulated the gene expressions related to intestinal immunity and barrier functions, such as IL17A, IL17F, IL10, cathelicidin-2 and pIgR. Body weights, and Claudin-1 and IL10 expressions were positively correlated (r = 0.41, p < 0.05 and r = 0.37, p = 0.06, respectively), whereas lesion scores of the small intestine and IL17A expression were negatively correlated (r = −0.33, p = 0.09). The microbiota analysis detected that genus Alistipes was more abundant in WC-supplemented broilers than in control, and positively correlated with body weights and Claudin-1 expression (r = 0.61, p < 0.05 and r = 0.51, p < 0.05, respectively). Intriguingly, genus Enterococcus was most abundant in WC-supplemented broilers and positively correlated with IL17A expression (r = 0.49, p < 0.05). Interestingly, Escherichia-Shigella was significantly more abundant in the small intestinal digestas of AM-administered broilers than in those of control. To summarize, WC supplementation modulated and immunostimulated the microbiotas of broilers, specifically genera Alistipes and Enterococcus, which led to the improvement of weight gain and coccidial symptoms, without disrupting the intestinal microbiota compositions, as AM did.

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Effects of mixed Eimeria challenge on performance, body composition, intestinal health, and expression of nutrient transporter genes of Hy-Line W-36 pullets (0-6 wks of age)

Cited by 25 publications

References 36 publications

Akkermansia muciniphila Promotes Bone Development and Improves Eggshell Quality during the Sexual Maturity Period of Laying Hens by Increasing Osteogenesis

Akkermansia muciniphila Promotes Bone Development and Improves Eggshell Quality during the Sexual Maturity Period of Laying Hens by Increasing Osteogenesis

The effects of arginine and branched-chain amino acid supplementation to reduced-protein diet on intestinal health, cecal short-chain fatty acid profiles, and immune response in broiler chickens challenged with Eimeria spp.

Dietary Weizmannia coagulans Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut Microbiota

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