Effects of miRNA-130a on the proliferation and apoptosis of glioma cell lines

Lu, Ke; Shen, Hechun; Zhu, Sheng Jie; Bi, Shui-Qing; Wu, Shengtian

doi:10.3892/ol.2018.8878

Cited by 7 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glioma, the most common primary brain tumor, is one of the most deadly malignancies in humans [ 1 – 3 ]. Since currently available treatments, including surgical resection, radiation and chemotherapy, are far from effective, patients with high-grade gliomas suffer from extremely poor prognosis and survival rates [ 4 – 6 ], with the average survival time at 9-12 months [ 7 ]. Genetic heterogeneity is a basic characteristic of glioma that causes abnormal up/downregulation in various signaling pathways [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation

Gao

Yang

Wang

et al. 2020

Aging

View full text Add to dashboard Cite

Glioma is the most common human primary brain cancer with high mortality and unfavorable clinical outcome. Coagulation factor 2 thrombin receptor (F2R), is a key component in the thrombosis process and has been demonstrated upregulated in various cancers. However, the effect and molecular mechanisms of F2R in glioma remains unclear. In our study, we confirmed that the expression of F2R was upregulated in glioma and predicted poor prognosis. Gene Set Enrichment Analysis (GSEA) and function assays demonstrated that F2R overexpression promoted glioma cell proliferation, metastasis and epithelial-mesenchymal transition (EMT) in vitro and tumor growth in vivo. Then, we identified and validated F2R was the target gene of SRY-box 2 (SOX2) by dual luciferase reporter assay and chromatin immunoprecipitation assay. Besides, High expression of F2R in malignant glioma was associated with β-catenint signaling pathway activation. Our findings conclude that F2R promotes glioma cell proliferation and metastasis under SOX2 and actives WNT/β-catenin Signaling pathway, which provides novel insight to the therapeutic regimen in glioma.

show abstract

Section: Introductionmentioning

confidence: 99%

Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation

Gao

Yang

Wang

et al. 2020

Aging

View full text Add to dashboard Cite

show abstract

“…As a multi-functional factor, miRNA-130a participates in various cellular activities in cancer cells, for example, autophagy, 63 proliferation, 64 and apoptosis. 65 MiRNA-130a has been reported to be an oncogenic factor, whose upregulation in NSCLC tissue is positively correlated to poor prognosis, lymph node metastasis, and stage of tumor node metastasis. 66 Meanwhile, miRNA-130a has been reported to play an important role in chemoresistance of various cancer types.…”

Section: Discussionmentioning

confidence: 99%

CAFs-Derived Exosomal miRNA-130a Confers Cisplatin Resistance of NSCLC Cells Through PUM2-Dependent Packaging

Zhang¹,

Zhang²

2021

IJN

View full text Add to dashboard Cite

Purpose Chemoresistance is a significant barrier to the treatment and management of non-small cell lung cancer (NSCLC). Exosomes play an essential role in intercellular communication. Understanding the mechanism underlying the role of tumor stroma, especially cancer-associated fibroblasts (CAFs), during chemoresistance would significantly contribute to the clinical application of chemotherapy agents. Results In this study, we demonstrated that NSCLC-derived CAFs were innately resistant to cisplatin treatment and CAFs-conditioned medium significantly promoted the survival rate of NSCLC cells after cisplatin treatment. Additionally, CAFs-derived exosomes were taken up by NSCLC cells. Moreover, exosomal miRNA-130a was transferred from CAFs to recipient NSCLC cells and knockdown of miRNA-130a reversed the effect of CAFs-derived exosomes during chemoresistance of NSCLC cells. Furthermore, pumilio homolog 2 (PUM2), a RNA-binding protein, mediated the packaging of miRNA-130a into exosomes. The overexpression and knockdown of PUM2 promoted and inhibited tumor growth of xenograft mice, respectively. Conclusion Taken together, these results suggest that CAFs-derived exosomes confer cisplatin resistance of NSCLC cells through transferring miRNA-130a and that PUM2 is a critical factor for packaging miRNA-130a into exosomes. This study indicates that CAFs-derived exosomal miRNA-130a may be a potential therapeutic target for cisplatin resistance in NSCLC.

show abstract

“…Therefore, in order to seek more effective treatment strategies, contemporary studies pay attention to the molecular mechanism involved in the genetic or epigenetic process of NSCLC . Recent tumor research has proved that miRNA is able to regulate various biological processes such as apoptosis, proliferation and metastases . Previous studies have elucidated that the alterations of miRNA expressions could influence NSCLC tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

microRNA‐100 functions as a tumor suppressor in non‐small cell lung cancer via regulating epithelial‐mesenchymal transition and Wnt/β‐catenin by targeting HOXA1

et al. 2020

View full text Add to dashboard Cite

Background: Non-small cell lung cancer (NSCLC) is a leading subtype in lung cancer, with high morbidities and mortalities worldwide. microRNA (miRNA) has appeared to play indispensable roles in a variety of solid carcinomas. The current study focused on the functions of miR-100 in NSCLC. Methods: qRT-PCR was performed to detect miR-100 and HOXA1 expressions in NSCLC tissues and cells. MTT and transwell assays were used to determine the functions of miR-100 in NSCLC cell proliferation, invasion and migration abilities. Western blot was used to measure related protein expressions. Results: qRT-PCR results showed that miR-100 expressions were dramatically decreased in NSCLC tissues. MTT assays indicated that miR-100 restoration inhibited NSCLC cell proliferation. Furthermore, transwell assay was performed to determine the impacts of miR-100 on NSCLC invasion and migration abilities. As expected, the invasion and migration capacities were significantly repressed. Direct interactions between HOXA1 and miR-100 were also verified via dualluciferase reporter assays. Western blot analysis demonstrated that miR-100 exerted suppressive functions via regulating EMT and Wnt/β-catenin in NSCLC cells. Conclusions: Our results showed that miR-100 served antitumor roles in NSCLC, providing new evidence of miR-100 as a promising therapeutic biomarker in NSCLC.

show abstract

Effects of miRNA-130a on the proliferation and apoptosis of glioma cell lines

Cited by 7 publications

References 19 publications

Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation

Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation

CAFs-Derived Exosomal miRNA-130a Confers Cisplatin Resistance of NSCLC Cells Through PUM2-Dependent Packaging

microRNA‐100 functions as a tumor suppressor in non‐small cell lung cancer via regulating epithelial‐mesenchymal transition and Wnt/β‐catenin by targeting HOXA1

Contact Info

Product

Resources

About