Effects of miR-31 on the osteogenesis of human mesenchymal stem cells

Xie, Qing; Wang, Zi; Bi, Xiaoping; Zhou, Huifang; Wang, Yefei; Gu, Ping; Fan, Xianqun

doi:10.1016/j.bbrc.2014.02.058

Cited by 68 publications

(55 citation statements)

References 21 publications

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“…After 7 days of osteogenic induction, hPDLSCs were fixed and stained following the protocol provided by the BCIP/NBT alkaline phosphatase colour development kit (Beyotime Biotechnology, China) for 30 minutes. ALP activity of hPDLSCs was measured according to the manufacturer's instructions using the Alkaline Phosphatase Assay kit (Beyotime Biotechnology) . Cells were incubated for 30 minutes in the dark.…”

Section: Methodsmentioning

confidence: 99%

Oestrogen retains human periodontal ligament stem cells stemness in long‐term culture

Wang

et al. 2017

Cell Proliferation

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Objectives: During long-term culture, loss of stemness is observed which greatly restricts the application of human periodontal ligament stem cells (hPDLSCs) in tissue regeneration. Oestrogen (E2) was found to significantly enhance the proliferation and osteogenic differentiation capacity in mesenchymal stem cells. Therefore, in this study, we investigated effects of E2 on hPDLSCs stemness in long-term culture. Materials and methods:Effects of E2 on hPDLSCs stemness were systematically evaluated. To characterize underlying the mechanisms, its effects on PI3K/AKT signalling pathway were determined. Results:Our results showed that E2 was able to enhance the proliferation, modify cell cycle, up-regulate stemness-related genes expression, promote osteogenic differentiation and elevate the positive rate of CD146 and STRO-1 over 10 passages in hPDLSCs. Importantly, PI3K/AKT signing pathway might play a role in these effects. Conclusions:These findings suggest that E2 retains hPDLSCs stemness in long-term culture, which might enhance its application in tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Oestrogen retains human periodontal ligament stem cells stemness in long‐term culture

Wang

et al. 2017

Cell Proliferation

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“…The second phase of bone remodeling involves specialized mononuclear cells that prepare the bone surface and attract osteoblasts (Raggatt and Partridge, ). Differentiation of osteoblasts is regulated mainly by SATB2 and SP7/OSX, whose transcripts are suppressed by miR‐31 (Baglìo et al, ; Deng et al, ; Xie et al, ). Differentiated osteoblasts promote bone matrix formation.…”

Section: Mir‐31 Maintains Bone Homeostasis In the Adult Vertebrates Amentioning

confidence: 99%

Function and regulation of microRNA‐31 in development and disease

Stepicheva

Song

2016

Molecular Reproduction Devel

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SUMMARY MicroRNAs (miRNAs) are small noncoding RNAs that orchestrate numerous cellular processes both under normal physiological conditions as well as in diseases. This review summarizes the functional roles and transcriptional regulation of the highly evolutionarily conserved miRNA, microRNA-31 (miR-31). miR-31 is an important regulator of embryonic implantation, development, bone and muscle homeostasis, and immune system function. Its own regulation is disrupted during the onset and progression of cancer and autoimmune disorders such as psoriasis and systemic lupus erythematosus. Limited studies suggest that miR-31 is transcriptionally regulated by epigenetics, such as methylation and acetylation, as well as by a number of transcription factors. Overall, miR-31 regulates diverse cellular and developmental processes by targeting genes involved in cell proliferation, apoptosis, cell differentiation, and cell motility.

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“…Baglìo et al 17 identified 29 differentially expressed miRNAs in human bone marrow MSCs after osteoinduction. Another study investigated the role of miR‐31 in osteogenesis of human MSCs and found that this process was repressed by upregulation of miR‐31 but enhanced by miR‐31 downregulation and that miR‐31 functioned by targeting special AT‐rich sequence‐binding protein 2 ( SATB2 ) 18 . miR‐140‐5p was also found to suppress bone morphogenetic protein ( BMP )2–mediated osteogenesis of human MSCs 19 .…”

mentioning

confidence: 99%