Effects of miR-19b Overexpression on Proliferation, Differentiation, Apoptosis and Wnt/β-Catenin Signaling Pathway in P19 Cell Model of Cardiac Differentiation In Vitro

Qin, Da-Ni; Qian, Lei; Hu, Deliang; Yu, Zhangbin; Han, Song; Zhu, Chun; Wang, Xuejie; Hu, Xiaoshan

doi:10.1007/s12013-013-9516-9

Cited by 47 publications

(38 citation statements)

References 44 publications

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“…MiR 19b-3p expression could inhibit TF expression, which suggests that TF may be a target gene of miR-19b-3p. In another study, Qin et al (2013) found that miR-19b-3p may be associated with the Wnt signalling pathway in cardiac development but without showing the direct target genes. In the current study, STAT3 activation was negatively regulated by miR-19b-3p and miR-29c-3p in SH-SY5Y cells, suggesting that STAT3 may be a direct target gene of these miRNAs.…”

Section: Discussionmentioning

confidence: 97%

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

et al. 2017

Tohoku J. Exp. Med.

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MicroRNAs (miRNAs) are short noncoding RNA that participate in posttranscriptional gene regulation. However, little is understood about the roles of miRNAs in Alzheimer's disease (AD). In this study, we used next-generation sequencing on RNA extracted from the serum samples of 20 AD patients and 20 controls, yielding a total of 72 miRNAs with significantly changed expression levels. Among these candidates, we selected 9 miRNAs with most significant alteration in disease, and validated their expression levels using RT-qPCR analysis on serum samples from 45 AD patients and 40 control subjects. Thus, the serum levels of miR-146a-5p, 106b-3p, 195-5p, 20b-5p, and 497-5p were significantly higher, while those of miR-125b-3p, 29c-3p, 93-5p and 19b-3p were significantly lower in AD patients, compared with control subjects. Two miRNAs, miR-29c-3p and miR-19b-3p, were selected because both RNA deep-sequencing and q-PCR methods indicated lower serum levels of these miRNAs in AD patients. Computational analysis predicted that 3′-untranslated region of signal transduction and activator of transcription 3 (STAT3) mRNA is targeted both by miR-29c-3p and miR-19b-3p. Using SH-SY5Y human neuroblastoma cells, we showed that transfection with miR-29c-3p or miR-19b-3p inhibitor significantly increased STAT3 phosphorylation. Furthermore, Water maze test, which assesses the learning and memory deficits in rodents, showed that escape latency was significantly shorter in AD rats with overexpression of miR-29c-3p or miR-19b-3p than in control AD rats. These results suggest that miR-29c-3p or miR-19b-3p may contribute to the cognitive function. In conclusion, the serum levels of miR-29c-3p and miR-19b-3p are helpful biomarkers for AD.

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Section: Discussionmentioning

confidence: 97%

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

et al. 2017

Tohoku J. Exp. Med.

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“…miRNAs are important regulators of cardiovascular development, and dysregulation of their expression has been linked to cardiac diseases [245–247]. miR-19b overexpression study revealed its regulation of the left-right symmetry and cardiac development in zebrafish embryo by directly suppressing β - catenin ( CTNNB1 ) of the Wnt signaling pathway [248,249]. The deformed cardiac phenotype was partially rescued by lithium chloride treatment which inhibits GSK3β, leading to an accumulation of β-catenin [248].…”

Section: Microrna Regulation Of Wnt Signaling Pathways In the Contmentioning

confidence: 99%

microRNA regulation of Wnt signaling pathways in development and disease

Song

Nigam

Tektas

et al. 2015

Cellular Signalling

114

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Wnt signaling pathways and microRNAs (miRNAs) are critical regulators of development. Aberrant Wnt signaling pathways and miRNA levels lead to developmental defects and diverse human pathologies including but not limited to cancer. Wnt signaling pathways regulate a plethora of cellular processes during embryonic development and maintain homeostasis of adult tissues. A majority of Wnt signaling components are regulated by miRNAs which are small noncoding RNAs that are expressed in both animals and plants. In animal cells, miRNAs fine tune gene expression by pairing primarily to the 3′untranslated region of protein coding mRNAs to repress target mRNA translation and/or induce target degradation. miRNA-mediated regulation of signaling transduction pathways is important in modulating dose-sensitive response of cells to signaling molecules. This review discusses components of the Wnt signaling pathways that are regulated by miRNAs in the context of development and diseases. A fundamental understanding of miRNA functions in Wnt signaling transduction pathways may yield new insight into crosstalks of regulatory mechanisms essential for development and disease pathophysiology leading to novel therapeutics.

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“…Considering that AMI is a complex pathophysiological process with the involvement of various kinds of cells, the contribution of non cardiac-enriched miRNAs is worthy of equal attention. miR-19b is a member of the miR-17-92 cluster (miR-17/18a/19a/19b/20a/92a), which is located on chromosome 13 [22,23]. Previous studies have shown that miR-19b is involved in aging-associated heart failure and the positive regulation of cardiomyocyte hypertrophy and apoptosis by targeting atrogin-1 and MuRF-1 [24].…”

Section: Introductionmentioning

confidence: 99%

Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction

Wang¹,

Zhao²,

Liu³

et al. 2016

Cell Physiol Biochem

118

103

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Background/Aims: Recent studies have shown that circulating microRNAs (miRNAs) are emerging as promising biomarkers for cardiovascular diseases. This study aimed to determine whether miR-19b-3p, miR-134-5p and miR-186-5p can be used as novel indicators for acute myocardial infarction (AMI). Methods: To investigate the kinetic expression of the three selected miRNAs, we enrolled 18 patients with AMI and 20 matched controls. Plasma samples were collected from each participant, and total RNA was extracted. Quantitative real-time PCR and ELISA assays were used to investigate the expression of circulating miRNAs and cardiac troponin I (cTnI), respectively. Plasma samples from another age- and gender-matched cohort were collected to investigate the impact of medications for AMI on the expression of the selected miRNAs. Results: Levels of plasma miR-19b-3p, miR-134-5p and miR-186-5p were significantly increased in early stage of AMI. Plasma miR-19b-3p and miR-134-5p levels reached peak expression immediately after admission (T0), whereas miR-186-5p achieved peak expression at 4 h after T0. All of these times were earlier than the peak for cTnI (8 h after T0). In addition, all three miRNAs were positively correlated with cTnI. Receiver Operating Characteristic (ROC) analysis indicated that each single miRNA showed considerable diagnostic efficiency for predicting AMI. Furthermore, combining all three miRNAs in a panel increased the efficiency of distinguishing between patients with AMI and controls. Moreover, we found that heparin and medications for AMI did not impact the expression of these circulating miRNAs. Conclusion: Circulating miR-19b-3p, miR-134-5p and miR-186-5p could be considered promising novel diagnostic biomarkers for the early phase of AMI.

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Effects of miR-19b Overexpression on Proliferation, Differentiation, Apoptosis and Wnt/β-Catenin Signaling Pathway in P19 Cell Model of Cardiac Differentiation In Vitro

Cited by 47 publications

References 44 publications

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

Lower Serum Levels of miR-29c-3p and miR-19b-3p as Biomarkers for Alzheimer’s Disease

microRNA regulation of Wnt signaling pathways in development and disease

Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction

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