Effects of miR-124-3p Silencing on Neuronal Damage in the Hippocampus of Depression Rats by Regulating STAT3 Gene

Abstract

Objective. A large amount of evidence shows that the abnormal expression of miRNA plays an important role in the development of depression. Therefore, we investigated the effect of miR-124-3p on neuronal damage in the hippocampus of depression rats. Methods. The target genes of miR-124-3p were predicted by the database; the depression model was prepared by subcutaneous injection of corticosterone (CORT), and LV-miR-124-3p asponge lentiviral suspension was given to determine the weight of rats and open-field test, sugar preference experiment, Serum CORT, 5-HT, DA, and NE were measured, observe and record the behavior of rats, including behavior, diet, and hair. The expression of miR-124-3p, STAT3, Bcl-2, and Bax in rat hippocampus was measured. The rat hippocampal neuron cells were extracted and transfected with miR-124-3p inhibitor; the cells were cultured with CORT, and the cell survival rate was evaluated by MTT experiment, and the expressions of miR-124-3p, STAT3, Bcl-2, and Bax in the cells were detected. Luciferase reporter gene verifies the targeted regulation of miR-124-3p on STAT3. Results. Compared with depression rats, silencing miR-124-3p increased the weight of the rats, increased the number of open-field activities, and significantly improved the general state and pathological state of the rats. The sugar water preference rate was significantly increased, the CORT content in the serum of rats decreased significantly, and the levels of 5-HT, DA, and NE increased significantly. After the treatment of silencing miR-124-3p, the expression level of miR-124-3p was decreased, while the STAT3 mRNA and protein expression levels were increased. And the protein and mRNA expression levels of Bcl-2 were increased, and the Bax protein and mRNA expression were decreased. Cell experiments verified that silencing miR-124-3p increased cell survival, the expression level of miR-124-3p decreased remarkably, while the expression levels of STAT3 mRNA and protein increased significantly. Silencing miR-124-3p reversed the effects of CORT treatment on miR-124-3p and STAT3 in neuronal cells. The luciferase reporter gene experiment confirmed that miR-124-3p targets and regulates STAT3 expression. Conclusion. Silencing miR-124-3p may protect hippocampal neurons from damage in depression rats by upregulating STAT3 gene.

Long Noncoding RNA LINC00473 Ameliorates Depression-Like Behaviors in Female Mice by Acting as a Molecular Sponge to Regulate miR-497-5p/BDNF Axis

“…For instance, the expression of miR-146a was negatively correlated with the degree of depression [ 12 ]. Moreover, miR-124-3p expression was increased in the brain of the depressed patients, and similar results were obtained in the serum of depressed patients [ 13 ]. Additionally, miR-497 level was overexpressed in CUMS-induced rats [ 14 ].…”

Implication of Wnt/GSK-3β/β-Catenin Signaling in the Pathogenesis of Mood Disturbances Associated with Hyperthyroidism in Rats: Potential Therapeutic Effect of Naringin

“…Both DICER1 and miR-124 genes were upregulated in rats with hyperthyroidism in the current study. Previous studies have reported the upregulation of miR-124 in the brains of both depressed rats and humans. , Promoting hippocampal neuronal apoptosis and reducing BDNF expression, as reported later in the current study, could offer an explanation for the detrimental impact of miR-124 in the development of mood illnesses in hyperthyroidism …”

“…Previous studies have reported the upregulation of miR- 124 in the brains of both depressed rats and humans. 27,28 Promoting hippocampal neuronal apoptosis and reducing BDNF expression, as reported later in the current study, could offer an explanation for the detrimental impact of miR-124 in the development of mood illnesses in hyperthyroidism. 28 Activation of β-catenin by thyroid hormones was reported in different cells.…”

“…27,28 Promoting hippocampal neuronal apoptosis and reducing BDNF expression, as reported later in the current study, could offer an explanation for the detrimental impact of miR-124 in the development of mood illnesses in hyperthyroidism. 28 Activation of β-catenin by thyroid hormones was reported in different cells. 29 A preceding study investigated the effect of thyroid hormones on β-catenin signaling and reported upregulation of β-catenin and cyclin D-1 expression in colorectal cancer cells upon treatment with T4.…”

Retracted: Effects of miR‐124‐3p Silencing on Neuronal Damage in the Hippocampus of Depression Rats by Regulating STAT3 Gene

“…This article has been retracted by Hindawi, as publisher, following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of systematic manipulation of the publication and peer-review process.…”