Effects of Mineral Fibers on the Gene Expression of Proinflammatory Cytokines and Inducible Nitric-Oxide Synthase in Alveolar Macrophages.

Morimoto, Yasuo; Tsuda, Tohru; Hirohashi, Masami; Hori, Hajime; Ohgami, Akira; Yatera, Kazuhiro; Kim, Heung-Nam; Ding, Li; Kido, Masamitsu; Higashi, Toshiaki; Tanaka, Isamu

doi:10.2486/indhealth.37.329

Cited by 20 publications

(10 citation statements)

References 10 publications

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“…In this study, we used a concentration of 2 mg since in our previous long-term fiber inhalation study we found the maximum amount of dust deposited in the lung to be 2 mg regardless of the fiber type (Oyabu et al, 1997). In a further study we also demonstrated a correlation between the fibrotic potential of 4 types of fibers and the expression of fibrosis-related genes after the instillation of a 2-mg dose (Morimoto et al, 1999. Therefore, the dose instilled in the present study was the upper limit of the amount physiologically deposited in the lung.…”

Section: Discussionmentioning

confidence: 75%

Patterns of Histopathological Change Determined by the Point Counting Method and Its Application for the Hazard Assessment of Respirable Dust

Ogami

Morimoto

Oyabu

et al. 2004

Inhalation Toxicology

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We evaluate the morphometric point counting method (PCM) for qualitatively analyzing pulmonary inflammation and collagen deposits (i.e., fibrosis) in the assessment of the biological hazards of inhaled respirable particles at a realistic dose comparable to that of exposure in the work environment. Rats were exposed by intratracheal instillation to a 2-mg dose, which is close to the estimated overdose at which macrophage clearance is impared, of each of 3 kinds of particulate matter: crystalline silica, crocidolite asbestos, and titanium dioxide. The lung tissue was evaluated at 3 days, 1 wk, and 1, 3, and 6 mo after exposure. Digital images taken of the lung tissue after processing and staining of the lung sections were examined by the PCM under light microscopy. Evidence of inflammation along with progressive inflammatory changes occurred with crystalline silica and crocidolite, which are well-known hazardous particle types. In contrast, lung tissue from rats exposed to titanium dioxide particles demonstrated a decreasing pattern of histopathological change with increasing retention time. Differences in repair patterns of TiO(2) versus crocidolite and silica following the 2-mg dose exposure suggest that the PCM scoring system may be a useful and sensitive tool for qualitatively evaluating the biological hazards of new particle types, for which no toxicological information exists for low-dose exposure, by using the results from assessment of fibrogenic particle types (such as crocidolite and crystalline silica) as well as particle types with low toxicity (such as TiO(2)) as reference points.

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Section: Discussionmentioning

confidence: 75%

Patterns of Histopathological Change Determined by the Point Counting Method and Its Application for the Hazard Assessment of Respirable Dust

Ogami

Morimoto

Oyabu

et al. 2004

Inhalation Toxicology

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“…In lung injury caused by dusts, phagocytosis of the dust induces inflammation via free radicals, and persistent or progressive inflammation is likely to cause fibrosis and, in turn, tumors (Borm & Driscoll, 1996;Kim et al, 2007;Morimoto et al, 1999). Continued inflammation has been reported in an animal exposure model using asbestos and silica, and thus persistent inflammation is an important pathology in the formation of irreversible chronic lesions (Borm et al, 1996;Shacter & Weitzman, 2002).…”

Section: Introductionmentioning

confidence: 94%

Expression of cytokine-induced neutrophil chemoattractant in rat lungs by intratracheal instillation of nickel oxide nanoparticles

Nishi

Morimoto

Ogami

et al. 2009

Inhalation Toxicology

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Since nanoparticles easily agglomerate to form larger particles, it is important to maintain the size of their agglomerates at the nano-level to evaluate the harmful effect of the nanoparticles. We prevented agglomeration of nickel oxide nanoparticles by ultrasound diffusion and filtration, established an acute exposure model using animals, and examined inflammation and chemokine expression. The mass median diameter of nickel oxide nanoparticle agglomerates suspended in distilled water for intratracheal instillation was 26 nm (8.41 nm weighted average surface primary diameter). Male Wistar rats received intratracheal instillation of nickel oxide nanoparticles at 0.1 mg (0.33 mg/kg) or 0.2 mg (0.66 mg/kg), and were dissected 3 days, 1 week, 1 month, 3 months, and 6 months after the instillation. The control group received intratracheal instillation of distilled water. Three chemokines (cytokine-induced neutrophil chemoattractant-1 (CINC-1), CINC-2alphabeta, and CINC-3) in the lung tissue and bronchoalveolar lavage fluid (BALF) were determined by quantitative measurement of protein by ELISA. Both CINC-1 and CINC-2alphabeta concentration was elevated from day 3 to 3 months in lung tissue and from day 3 to 6 months in BALF. On the other hand, CINC-3 was elevated on day 3 in both lung tissue and BALF, and then decreased. The total cell and neutrophil counts in BALF were increased from day 3 to 3 months. In lung tissue, infiltration of mainly neutrophils and alveolar macrophages was observed from day 3 to 6 months in alveoli. These results suggest that CINC was involved in lung injury by nickel oxide nanoparticles.

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“…Die chronische Entzündung wird als treibender Prozess für weitere pathophysiologische Zell und Gewebever änderungen der Lunge angesehen (IARC 2002;ILSI 2005;NTP 2010). Die Schädi gung der Epithelzellen führt zu Hyperplasie und Hypertrophie und gelegentlich zu Tumorentstehung (Driscoll et al1995;Mossman und Churg 1998;Oberdörster und Lehnert 1991;Saffiotti 1998;Tsuda et al 1997 (Bermudez et al 2003;Creutzenberg et al 1997;IARC 2002;Morimoto et al 1999Morimoto et al , 2001NTP 2010;Schürkes et al 2004). Auch KohlenstoffNanoröhrchen induzieren chronische Entzündung bei den exponierten Tieren (Poland et al 2008).…”

Section: Chronische Entzündungunclassified

Faserstäube, anorganisch [MAK Value Documentation in German language, 2018]

Hartwig

Arand²

2018

The MAK‐Collection for Occupational Health and Safety

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The German Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area has reevaluated the general aspects of the toxicity of inorganic fibrous dusts. As a first step a documentation on the effects caused by inhalation of inorganic fibrous dusts and the mode of action for their toxicity was set up. For the elucidation of the mechanism of the toxicity and carcinogenicity of fibers, data derived from studies with asbestos fibers were also considered, in order to obtain some knowledge with regard to the biological effects of the other inorganic fibrous dusts. The formation of tumors in the lungs and on serous membranes is mainly the result of inflammatory processes. Chronic inflammation and cell proliferation are caused by the impairment of fiber clearance, whereby inflammation‐promoting cytokines, growth factors, reactive oxygen (ROS) and nitrogen species (RNS) and chlorine radicals are released from the macrophages, inflammatory cells, alveolar cells and mesothelial cells. The generation of these radicals leads to genotoxic effects. In addition intracellular formation of ROS and RNS can be caused by the fibers themselves, as a result of their own surface reactivity. Fibers can stimulate cell receptors and inflammasomes. Each fiber dust group will be evaluated individually and, depending on the data available, may be classified in one of the categories for carcinogens. The Commission has already begun this procedure.

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Effects of Mineral Fibers on the Gene Expression of Proinflammatory Cytokines and Inducible Nitric-Oxide Synthase in Alveolar Macrophages.

Cited by 20 publications

References 10 publications

Patterns of Histopathological Change Determined by the Point Counting Method and Its Application for the Hazard Assessment of Respirable Dust

Patterns of Histopathological Change Determined by the Point Counting Method and Its Application for the Hazard Assessment of Respirable Dust

Expression of cytokine-induced neutrophil chemoattractant in rat lungs by intratracheal instillation of nickel oxide nanoparticles

Faserstäube, anorganisch [MAK Value Documentation in German language, 2018]

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