Effects of Mild and Severe Vitamin B<sub>1</sub> Deficiencies on the Meiotic Maturation of Mice Oocytes

Tsuji, Akira; Nakamura, Toshinobu; Shibata, Katsumi

doi:10.1177/1178638817693824

Cited by 8 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There was a beneficial effect of these combined antioxidants on embryo development [ 78 ], and a positive correlation between the presence of these antioxidants and increased blastocyst cells number [ 79 ]. The evidences about the efficacy of these compounds, administered alone in culture medium, in increasing the oocytes fertilization rate [ 93 , 94 ] and in improving embryo development [ 95 , 96 , 97 , 98 , 99 ] has also been highlighted in experimental studies [ 93 , 94 , 95 , 96 , 97 , 98 , 99 ].…”

Section: Agents Consideredmentioning

confidence: 99%

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Budani

Tiboni

2020

Antioxidants

View full text Add to dashboard Cite

For most infertile couples, in vitro fertilization (IVF) represents the only chance to conceive. Given the limited success of IVF procedures, novel approaches are continuously tested with the aim of improving IVF outcomes. Growing attention is devoted today to the potential benefit of natural antioxidants in the optimization of infertility treatments. This review summarizes current data in this context, focusing on both experimental studies on oocytes/embryos and clinical trials on antioxidants supplementation. Based on information gained from experimental studies, antioxidant supplementation may have beneficial effects on IVF outcomes in terms of quality and cryotolerance of in vitro produced embryos, together with positive effects on in vitro maturation oocytes and on early embryonic development. Unfortunately, from the clinical side, there is a paucity of evidence favoring the protective qualities of antioxidants. Among the antioxidants considered, coenzyme Q10 may be regarded as one of the most promising for its positive role in rescuing the oxidative stress-induced damages, but further data are needed. It is concluded that further trials are necessary to characterize the potential clinical value of antioxidants in IVF treatments.

show abstract

Section: Agents Consideredmentioning

confidence: 99%

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Budani

Tiboni

2020

Antioxidants

View full text Add to dashboard Cite

show abstract

“…The oocytes were observed by confocal fluorescence microscopy. The method used to classify oocytes as normal or abnormal is described elsewhere [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Reduction of oocyte lipid droplets and meiotic failure due to biotin deficiency was not rescued by restoring the biotin nutritional status

et al. 2022

Self Cite

View full text Add to dashboard Cite

BACKGROUND/OBJECTIVES Oocyte lipid droplets play a crucial role in meiosis and embryo development. Biotin is associated with fatty acid synthesis and is the coenzyme for acetyl-CoA carboxylase (ACC). The effects of a biotin deficiency on the oocyte lipid metabolism remain unknown. This study examined the effects of a biotin deficiency and its replenishment on murine 1) oocyte lipid droplet levels, 2) ovary lipid metabolism, and 3) oocyte meiosis. MATERIALS/METHODS Mice were divided into 3 groups: control, biotin deficient (BD), and recovery groups. The control and BD groups were fed a control diet or BD diet (0.004 or 0 g biotin/kg), respectively. The recovery group mice were fed a BD diet until day 21, and were then fed the control diet from days 22 to 64. This study then quantified the oocyte lipid droplet levels, assessed the oocyte mitochondrial function, and examined the ability of oocytes to undergo meiosis. Ovarian phosphorylated ACC (p-ACC), lipogenesis, β-oxidation, and ATP production-related genes were evaluated. RESULTS The BD group showed a decrease in lipid droplets and mitochondrial membrane potential and increased p-ACC levels. In the recovery group, the hepatic biotin concentration, ovarian p-ACC levels, and mitochondrial membrane potential were restored to the control group levels. On the other hand, the quantity of lipid droplets in the recovery group was not restored to the control levels. Furthermore, the percentage of oocytes with meiotic abnormalities was higher in the recovery group than in the control group. CONCLUSIONS A biotin deficiency reduced the oocyte lipid droplet levels by downregulating lipogenesis. The decreased lipid droplets and increased oocyte meiosis failure were not fully restored, even though the biotin nutrition status and gene expression of lipid metabolism was resumed. These results suggest that a biotin deficiency remains robust and can be long-lasting. Biotin might play a crucial role in maintaining the oocyte quality.

show abstract

“…Purine metabolism plays a role in cell growth or division, with expression in the ovary tied to the cell proliferation of oocytes [50,51]. Thiamine metabolism plays a role in the activity of key enzymes in cellular metabolism and has been linked to the meiotic maturation of oocytes [52,53].…”

Section: Functional Annotation and Classificationmentioning

confidence: 99%

“…Purine metabolism is linked to oocyte quality, which can lead to lower fertility when there are changes to this metabolic pathway [51,76]. Similarly, thiamine metabolism has been identified as part of cellular metabolism and meiotic maturation of oocytes in mice [52,53]. Arthropods that are unable to sequester thiamine in their diets, such as tsetse flies and ticks, have reduced fertility when their symbiont-typically a bacteria that produces thiamine-is removed [79].…”

Section: Western Black Widow Spider Ovarian Gene and Pathway Categoriesmentioning

confidence: 99%

“…Arthropods that are unable to sequester thiamine in their diets, such as tsetse flies and ticks, have reduced fertility when their symbiont-typically a bacteria that produces thiamine-is removed [79]. The reduced fertility in these arthropods is likely caused by vitamin B deficiencies that can inhibit meiotic maturation of oocytes [53]. While this study suggests these pathways may be major factors in ovarian development, investigating these patterns (such as vitamin deficiencies) in other closely-related spiders and within L. hesperus will determine whether they play a unique role in this species.…”

Section: Western Black Widow Spider Ovarian Gene and Pathway Categoriesmentioning

confidence: 99%

See 1 more Smart Citation

Ovarian Transcriptomic Analyses in the Urban Human Health Pest, the Western Black Widow Spider

Miles

Ayoub

Garb

et al. 2020

Genes

View full text Add to dashboard Cite

Due to their abundance and ability to invade diverse environments, many arthropods have become pests of economic and health concern, especially in urban areas. Transcriptomic analyses of arthropod ovaries have provided insight into life history variation and fecundity, yet there are few studies in spiders despite their diversity within arthropods. Here, we generated a de novo ovarian transcriptome from 10 individuals of the western black widow spider (Latrodectus hesperus), a human health pest of high abundance in urban areas, to conduct comparative ovarian transcriptomic analyses. Biological processes enriched for metabolism—specifically purine, and thiamine metabolic pathways linked to oocyte development—were significantly abundant in L. hesperus. Functional and pathway annotations revealed overlap among diverse arachnid ovarian transcriptomes for highly-conserved genes and those linked to fecundity, such as oocyte maturation in vitellogenin and vitelline membrane outer layer proteins, hormones, and hormone receptors required for ovary development, and regulation of fertility-related genes. Comparative studies across arachnids are greatly needed to understand the evolutionary similarities of the spider ovary, and here, the identification of ovarian proteins in L. hesperus provides potential for understanding how increased fecundity is linked to the success of this urban pest.

show abstract

Effects of Mild and Severe Vitamin B₁ Deficiencies on the Meiotic Maturation of Mice Oocytes

Cited by 8 publications

References 35 publications

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Reduction of oocyte lipid droplets and meiotic failure due to biotin deficiency was not rescued by restoring the biotin nutritional status

Ovarian Transcriptomic Analyses in the Urban Human Health Pest, the Western Black Widow Spider

Contact Info

Product

Resources

About

Effects of Mild and Severe Vitamin B1 Deficiencies on the Meiotic Maturation of Mice Oocytes

Cited by 8 publications

References 35 publications

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Reduction of oocyte lipid droplets and meiotic failure due to biotin deficiency was not rescued by restoring the biotin nutritional status

Ovarian Transcriptomic Analyses in the Urban Human Health Pest, the Western Black Widow Spider

Contact Info

Product

Resources

About

Effects of Mild and Severe Vitamin B₁ Deficiencies on the Meiotic Maturation of Mice Oocytes