Effects of Microcystis on development of early life stage Japanese medaka (Oryzias latipes): Comparative toxicity of natural blooms, cultured Microcystis and microcystin-LR

Saraf, Spencer R.; Frenkel, Amy; Harke, Matthew J.; Jankowiak, Jennifer G.; Gobler, Christopher J.; McElroy, Anne E.

doi:10.1016/j.aquatox.2017.10.026

Cited by 58 publications

(22 citation statements)

References 44 publications

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“…The changes we describe herein include oedema in the abdominal region, mainly in the heart and digestive system. Similar results were also reported in other studies [54,55,58,67,68]. Tissue haemorrhage was noticed in some samples and was probably caused by the presence of MC-LR, LY and HilR variants produced by MIRS 04 strain (Figure 7).…”

Section: Discussionsupporting

confidence: 90%

“…Under stressful conditions, metabolic changes are expected in response to different environmental variations and the development of tissue oedema, size and shape modification are effects previously reported for several aquatic organisms [56]. In the initial stage of fish development, these effects may compromise survival since larvae are more sensitive to toxic compounds in freshwater ecosystems [68,69,70,71,72,73].…”

Section: Discussionmentioning

confidence: 99%

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Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae

Fernandes

Gomes

Calado

et al. 2019

Toxins

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Absorption and accumulation of bioavailable cyanobacterial metabolites (including cyanotoxins) are likely in fish after senescence and the rupturing of cells during bloom episodes. We determined the toxicity of cyanopeptides identified from two strains of Microcystis (M. panniformis MIRS-04 and M. aeruginosa NPDC-01) in a freshwater tropical fish, Astyanax altiparanae (yellowtail tetra, lambari). Aqueous extracts of both Microcystis strains were prepared in order to simulate realistic fish exposure to these substances in a freshwater environment. Both strains were selected because previous assays evidenced the presence of microcystins (MCs) in MIRS-04 and lack of cyanotoxins in NPDC-01. Identification of cyanobacterial secondary metabolites was performed by LC-HR-QTOF-MS and quantification of the MC-LR was carried out by LC-QqQ-MS/MS. MIRS-04 produces the MCs MC-LR, MC-LY and MC-HilR as well as micropeptins B, 973, 959 and k139. NPCD-01 biosynthetizes microginins FR1, FR2/FR4 and SD-755, but does not produce MCs. Larval fish survival and changes in morphology were assessed for 96 h exposure to aqueous extracts of both strains at environmentally relevant concentrations from 0.1 to 0.5 mg (dry weight)/mL, corresponding to 0.15 to 0.74 μg/mL of MC-LR (considering dried amounts of MIRS-04 for comparison). Fish mortality increased with concentration and time of exposure for both strains of Microcystis. The frequencies of morphological abnormalities increased with concentration in both strains, and included abdominal and pericardial oedema, and spinal curvature. Results demonstrate that toxicity was not solely caused by MCs, other classes of cyanobacterial secondary metabolites contributed to the observed toxicity.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae

Fernandes

Gomes

Calado

et al. 2019

Toxins

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“…Natural cyanobacterial blooms, lab cultures, biomass extracts and pure microcystins (e.g. MC-LR) were shown to adversely impact its development, reproduction, and to induce specific organ alterations, notably hepatotoxicity and cardiotoxicity 7,[13][14][15] . However, despite the documented impact of cyanobacterial blooms on fish health, no study has to our knowledge investigated the link between these events and the composition of fish microbiota.…”

Section: Introductionmentioning

confidence: 99%

Response of Fish Gut Microbiota to Toxin-Containing Cyanobacterial Extracts: A Microcosm Study on the Medaka (Oryzias latipes)

Duperron

Halary

Habiballah

et al. 2019

Environ. Sci. Technol. Lett.

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The effect of toxin-producing cyanobacterial blooms on fishes health has been investigated extensively, but no study to date has evaluated this effect on fish-associated microbiota. In this study, we test the effect of pure microcystins and of crude extracts of metabolites from Microcystis aeruginosa cultures on the composition of gut bacterial microbiota in Medaka fishes (Oryzias latipes) exposed for 28 days in a microcosm experiment. A 16S rRNA-based marker gene-based approach was used to investigate the composition of bacterial communities. Results show that fish gut community compositions differ from those occurring in the water, and among individual fishes. Exposure to extracts, rather than pure microcystin, has a significant influence on gut community composition, with a marked increase in relative abundances of pathogen-related bacteria (genera Nocardia and Mycobacterium) in the presence of one extract, and of bacterial orders Sphingomonadales and Saprospirales in the other. We suggest that compounds identified in the cyanobacterial extracts, but not microcystin LR alone, alter the composition of bacterial communities, with possible consequences for various biological functions in fishes. This pioneer microcosm experiment indicates that cyanobacterial blooms probably have an effect on fish gut microbiota and associated functions, including toxin degradation and feed efficiency, and should be further explored.

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“…To date, a large number of studies have shown that MCs can induce cardiovascular toxicity in vivo and vitro directly [36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53]. Table 1 and Table 2 represent summaries of cardiovascular toxicity in in vivo and vitro studies, respectively.…”

Section: Cardiovascular Toxicity Of Mcsmentioning

confidence: 99%

A Review of Cardiovascular Toxicity of Microcystins

Cao

Massey

Feng

et al. 2019

Toxins

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The mortality rate of cardiovascular diseases (CVD) in China is on the rise. The increasing burden of CVD in China has become a major public health problem. Cyanobacterial blooms have been recently considered a global environmental concern. Microcystins (MCs) are the secondary products of cyanobacteria metabolism and the most harmful cyanotoxin found in water bodies. Recent studies provide strong evidence of positive associations between MC exposure and cardiotoxicity, representing a threat to human cardiovascular health. This review focuses on the effects of MCs on the cardiovascular system and provides some evidence that CVD could be induced by MCs. We summarized the current knowledge of the cardiovascular toxicity of MCs, with regard to direct cardiovascular toxicity and indirect cardiovascular toxicity. Toxicity of MCs is mainly governed by the increasing level of reactive oxygen species (ROS), oxidative stress in mitochondria and endoplasmic reticulum, the inhibition activities of serine/threonine protein phosphatase 1 (PP1) and 2A (PP2A) and the destruction of cytoskeletons, which finally induce the occurrence of CVD. To protect human health from the threat of MCs, this paper also puts forward some directions for further research.

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Effects of Microcystis on development of early life stage Japanese medaka (Oryzias latipes): Comparative toxicity of natural blooms, cultured Microcystis and microcystin-LR

Cited by 58 publications

References 44 publications

Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae

Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae

Response of Fish Gut Microbiota to Toxin-Containing Cyanobacterial Extracts: A Microcosm Study on the Medaka (Oryzias latipes)

A Review of Cardiovascular Toxicity of Microcystins

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