Effects of microcurrent application alone or in combination with topical Hypericum perforatum L. and Arnica montana L. on surgically induced wound healing in Wistar rats

Castro, Fabiene C.B.; Magre, Amanda; Cherpinski, Ricardo; Zelante, Paulo M.; Neves, Lia Mara Grosso; Esquisatto, Marcelo Augusto Marretto; Mendonça, Fernanda Aparecida Sampaio; Santos, Gláucia Maria Tech dos

doi:10.1016/j.homp.2012.05.006

Cited by 42 publications

(34 citation statements)

References 37 publications

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“…The Asteraceae family is well known for its therapeutic properties both in folk medicine and across different studies [29,75,76]. Thus, we decided to investigate and contribute to the knowledge of the therapeutic effects of S. chilensis, a Brazilian arnica that is easily accessible and widely used in folk medicine, but little is known scientifically.…”

Section: Discussionmentioning

confidence: 99%

“…Medicinal plants are frequently used to treat skin disorders, particularly cut, wound and burn [26], and many phytotherapeutic agents have shown efficacy in tissue repair [27][28][29]. Solidago chilensis Meyen (family Asteraceae) is a plant native of South America, including southern and southeastern Brazil and is commonly known as Brazilian arnica.…”

Section: Introductionmentioning

confidence: 99%

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InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Catarino¹,

Godoy²,

Scharlack³

et al. 2015

Lasers Med Sci

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Therapies that accelerate the healing of burn injuries, improving the quality of life of the patient and reducing the cost of treatment are important. This study evaluated the effects of InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis leaves on burn wound healing in rats. Seventy-two rats were divided randomly into four groups: control untreated (C), treated with InGaP 670-nm laser with power density of 0.41 W/cm(2) and energy density of 4.93 J/cm(2) (L), treated with S. chilensis extract (S) and treated with S. chilensis extract and laser (LS). Second-degree burns were produced on the back of the animals with metal plate. Wound samples were collected on days 7, 14 and 21 of treatment for structural analysis, morphometry and Western blotting to quantify the expression of transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor (VEGF). The results showed that InGaP laser irradiation at 670 nm alone and combined with extract of S. chilensis promoted significant tissue repair responses in this experimental model, increasing the number of fibroblasts, collagen fibres and newly formed blood vessels throughout the experimental period and decreasing the number of granulocytes in burn wounds of second degree in all treated groups. Exclusive treatment of burn wounds with the hydroalcoholic extract of S. chilensis provided similar quantitative results to those seen in the untreated group throughout the experimental period. Therefore, it was observed in the L and LS groups different responses in the expression of TGF-β1 and VEGF. The application of 670-nm laser alone or combined with the extract of S. chilensis promoted favourable responses in tissue repair of second-degree burns in this experimental model.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Catarino¹,

Godoy²,

Scharlack³

et al. 2015

Lasers Med Sci

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show abstract

“…In a study by Süntar et al [8] , it was found that active HP extracts enhanced re-epithelization, neovascularization and the proliferation capacity of fibroblasts. In a study using a wound-healing model, Castro et al [20] found that cell migration was increased around the wound site on days 6 and 10. The authors also found that tissue reorganization and collagen fibril maturation were increased prominently on day 6.…”

Section: Discussionmentioning

confidence: 99%

Potential Curative Role of Hypericum Perforatum in an Experimental Rat Model of Tympanic Membrane Perforation

Yaşar¹,

Kaya²,

Karaman³

et al. 2016

Int Adv Otol

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OBJECTIVE:Our aim was to investigate the curative role of topical Hypericum perforatum (HP) in a rat model of tympanic membrane perforation in a histopathological manner. MATERIALS AND METHODS:This study was conducted on 30 female Sprague-Dawley rats. An HP extract was prepared as a suspension in pure olive oil. In all rats, the right and left tympanic membranes were perforated by a fine puncture under a microscope. Rats were randomly assigned to three groups. The HP extract was given as drops on a daily basis in Group 1, while olive oil alone was given on a daily basis in Group 2. In Group 3, the rats were allowed spontaneous recovery without any intervention. Three rats from each group were sacrificed on days 7, 14, and 21. Temporal bullae were removed for histopathological examination to evaluate fibroblast proliferation, leukocyte infiltration, neovascularization, and subepithelial thickness and to compare these among the groups. RESULTS:In histopathological examination of the rats from each group on days 7, 14, and 21, there were significant differences in leukocyte infiltration, fibroblast proliferation, and subepithelial thickness. No significant difference was observed in neovascularization among the groups. CONCLUSION:Compared with the spontaneous recovery group, HP was found to be more effective in a wound-healing model of the tympanic membrane. HP may be applied in clinical practice if it is shown to be safe with regard to ototoxicity. comfortable and practical treatment protocol. In attempts to find a way for a better solution of this problem, experimental wound models on the tympanic membrane of rats have been applied and the results published. Previous studies have revealed the positive and negative effects of various agents on the healing of tympanic membranes, including hyaluronic acid, epidermal growth factor, transforming growth factor, platelet-derived growth factor, and platelet-rich plasma [10][11][12][13] . KEYWORDS:To the best of our knowledge, there are no data showing that HP has been used for healing tympanic membranes.Therefore, we aimed to assess the effect of HP olive oil extract on the wound-healing process in experimentally perforated tympanic membranes of rats. MATERIALS AND METHODS AnimalsThis study was conducted on 30 female Sprague-Dawley rats aged ≥6 weeks, weighing 200-300 g. The rats were fed with standard rat pellet and tap water ad libitum. Rats were housed in separate cages (five rats per cage) under standard conditions at a temperature of 21°C and maintaining a 12-hour dark-light cycle. This study was approved by the Ethics Committee on Animal Experiment and Research (2013-13/ 112). Experimental ProcedureHypericum perforatum was harvested from rural areas of Adana Province in July and then dried. In the laboratory, 50 g of dry HP was weighed. The weighed HP was immersed in 500 cc of olive oil in glass jars and left in the sunshine over 4 weeks until the occurrence of a red coloration. There is also a traditional recipe for this: "the content of the jar should be exposed ...

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“…Some previous studies have demonstrated that Arnica montana exhibits potential anti-inflammatory activity. [11][12][13][14][15] Additionally, other studies have demonstrated the beneficial effects of Arnica montana in wound healing, [16][17][18] pain relief, 19 antioxidant activity and cytoprotective effect against oxidative damage. 20 However, to our knowledge the mechanism by which Arnica montana acts on the oral wounds is not known.…”

Section: ■ Discussionmentioning

confidence: 99%

Arnica montana does not affect mast cell populations in experimentally induced oral ulcers in rats

Perussolo¹,

Grégio²,

Azevedo‐Alanis³

et al. 2015

Medical Express

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BACKGROUND:Studies have shown that Arnica montana shows anti-inflammatory and antioxidant activities. It has been used in traditional medicine for the treatment of several disorders. The aim of this study was to investigate the effect of Arnica montana on mast cells during the wound healing of oral ulcers. METHOD: An ulcerated lesion was chemically induced on the tongue of 75 male albino rats and, then, treated topically for seven days using saline solution (control), Arnica montana gel or tincture. The animals were killed after 2

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Effects of microcurrent application alone or in combination with topical Hypericum perforatum L. and Arnica montana L. on surgically induced wound healing in Wistar rats

Cited by 42 publications

References 37 publications

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Potential Curative Role of Hypericum Perforatum in an Experimental Rat Model of Tympanic Membrane Perforation

Arnica montana does not affect mast cell populations in experimentally induced oral ulcers in rats

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