Effects of microbial elicitor on production of hypocrellin by Shiraia bambusicola

Abstract

Some endophyte isolates were isolated in a bamboo pole sample parasitized the fungus Shiraia bambusicola from Zhejiang Province. After screening through hypocrellin bacteriostatic effect and fermentation test, we got the isolate TX4 of bacterial elicitor and GZUIFR-TT1 of fungal elicitor which had certain effect to promote S. bambusicola to produce hypocrellin. The Plackett-Burman design was introduced to evaluate the effects of nine factors based on single-factor test. Yeast extract, glucose, and isolate GZUIFR-TT1 elicitor were found to be the critical activity factors for increasing the total hypocrellin production. So we identified the isolate GZUIFR-TT1 as Trametes sp. Through response surface methodology, we obtained the optimum production conditions as follows: yeast extract, 2.99 g/L; glucose, 32.45 g/L; and Trametes sp. elicitor, 81.40 μg/mL. Under the above conditions, the experimental value of hypocrellin production was 102.60 mg/L, compared with the control it increased about 7.90 times.

Nitric oxide mediates hypocrellin accumulation induced by fungal elicitor in submerged cultures of Shiraia bambusicola

“…The determination of hypocrellin content was similar to the method described by Du et al (2013). The dried mycelia (100 mg) were treated in 6 M HCl at 60°C (1 h), and the precipitate was collected by centrifugation and extracted by ethanol (5 ml) for 24 h (twice).…”

“…Increases in hypocrellin production induced by fungal elicitors and the mechanism involved are rarely investigated. Our previous study showed that the endophytic fungus Trametes sp., which was isolated from the endothelial stem cells of bamboo, improves the survival rate of S. bambusicola cultures and increases hypocrellin expression (Du et al 2013). Similar reports are rarely available.…”

Improvement of hypocrellin production by a new fungal source and optimization of cultivation conditions

“…[20] Furthermore, it has been proved that addition of bacterial and fungal elicitor into a Czapek medium is effective for advancing the efficiency of fermentation process. [19] In the present study, we also optimized the cultivation condition and culture medium composition, and the results displayed that HA increased remarkably by the appropriate parameters for the liquid fermentation.…”

“…Microbial elicitors had taken effect on the production of hypocrellin by the wild isolates of S. bambusicola, and the experimental value of hypocrellin production was 102.60 mg/L. [19] Compared with the published achievements, our optimum content of hypocrellin is significantly high among them, which raised the prospect that a new S. bambusicola strain ZZZ816 is of great potential for reducing the cost of hypocrellin production and expanding the application range.…”

“…[12,13] Many researches aimed to enhance the production of hypocrellins from mycelium, including screening of industrial strains, [12,14,15] optimization of extraction technology, [16,17] control of fermentation process [13,18] and search of microbial elicitors. [19,20] The fermentation process takes a vital part in the yield of the ultimate products, and generally requires a statistical method to analyse the effects of multiple variables simultaneously, for minimizing the assay time, cutting the cost of experiments, optimizing the procedure of mycelium cultivation and saving manual labour. Orthogonal array design (OAD) is a type of fractional factorial design, where the series of trials were assigned by an orthogonal array.…”