Effects of micro-aeration on microbial niches and antimicrobial resistances in blackwater anaerobic digesters

Guo, Bing; Yu, Najiaowa; Weissbrodt, David G.; Liu, Yang

doi:10.1016/j.watres.2021.117035

Cited by 44 publications

(14 citation statements)

References 77 publications

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“…A growing body of research has indicated that the resistome is mainly determined by microbial community composition, − although the resistome was once thought not to correlate with phylogeny or taxonomy due to the high mobility of ARGs. − To investigate the relationship between the microbial community and ARG hosts in our reactors, the microbial community structure was analyzed based on the presence of the 16S rRNA gene in metagenomic data, and the ARG hosts of both binned and unbinned ARGs were predicted and counted. There were 115, 118, 130, 119, and 101 genera identified as ARG hosts from ck, r0, r1, r2, and r3, respectively (Dataset S2).…”

Section: Results and Discussionmentioning

confidence: 99%

Biological Mitigation of Antibiotic Resistance Gene Dissemination by Antioxidant-Producing Microorganisms in Activated Sludge Systems

Ren

Hartmann

et al. 2021

Environ. Sci. Technol.

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Antibiotic resistance is the principal mechanism of an evergrowing bacterial threat. Antibiotic residues in the environment are a major contributor to the spread of antibiotic resistance genes (ARGs). Subinhibitory concentrations of antibiotics cause bacteria to produce reactive oxygen species (ROS), which can lead to mutagenesis and horizontal gene transfer (HGT) of ARGs; however, little is known about the mitigation of ARG dissemination through ROS removal by antioxidants. In this study, we examine how antioxidant-producing microorganisms inoculated in replicate activated sludge systems can biologically mitigate the dissemination of ARGs. Through quantitative polymerase chain reaction (qPCR), we showed that antioxidant-producing microorganisms could decrease the persistence of the RP4 plasmid and alleviate enrichment of ARGs (sul1) and class 1 integrons (intl1). Metagenomic sequencing identified the most diverse resistome and the most mutated Escherichia coli ARGs in the reactor that contained antibiotics but no antioxidant-producing microorganisms, suggesting that antioxidant-producing microorganisms mitigated ARG enrichment and mutation. Host classification revealed that antioxidant-producing microorganisms decreased the diversity of ARG hosts by shaping the microbial community through competition and functional pathway changes. Conjugative experiments demonstrated that conjugative transfer of ARGs could be mitigated by coculture with antioxidant-producing microorganisms. Overall, this is a novel study that shows how ARG enrichment and HGT can be mitigated through bioaugmentation with antioxidant-producing microorganisms.

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Section: Results and Discussionmentioning

confidence: 99%

Biological Mitigation of Antibiotic Resistance Gene Dissemination by Antioxidant-Producing Microorganisms in Activated Sludge Systems

Ren

Hartmann

et al. 2021

Environ. Sci. Technol.

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“…Additionally, the micro-aerobic condition also benefited fermentative bacteria. 33 Abundant fermentative Chloroflexi and Bacteroidetes under micro-aerobic conditions provide usable organics for denitrifiers and favor the granulation process, which provides anoxic living space for both AnAOB and denitrifiers. In return, AnAOB could generate SMP and vitamins to support the growth of heterotrophic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Insight into the characteristics of microbial communities in a single-stage anammox reactor under different oxygen conditions

et al. 2022

Environ. Sci.: Water Res. Technol.

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“…When sufficient dissolved oxygen is present, aerobic microorganisms oxidatively decompose the organic matter in wastewater. Studies have shown that aeration was beneficial for the reduction of ARGs ( 38 , 39 ). Another study found that the abundance of total ARGs was decreased by 1.77 log copies/mL during aeration of the tank, but a change in sul1 abundance was not observed ( 40 ).…”

Section: Efficacy Of Arg Removal By Livestock Wastewater Treatment Pr...mentioning

confidence: 99%

Removal of antibiotic resistance genes during livestock wastewater treatment processes: Review and prospects

Huang

Hong

et al. 2022

Front. Vet. Sci.

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Antibiotic resistance genes (ARGs) are emerging pollutants that have received extensive attention. Many different types of ARGs exist in livestock wastewater. If not effectively treated, they can threaten animal production, public health and the ecological safety of the surrounding environment. To address the high risk of livestock wastewater contamination by ARGs, the effects of different wastewater treatment processes on ARGs and their influencing factors and mechanisms are reviewed herein. Additionally, the current problems associated with removal of ARGs are discussed, and future research is proposed.

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Effects of micro-aeration on microbial niches and antimicrobial resistances in blackwater anaerobic digesters

Cited by 44 publications

References 77 publications

Biological Mitigation of Antibiotic Resistance Gene Dissemination by Antioxidant-Producing Microorganisms in Activated Sludge Systems

Biological Mitigation of Antibiotic Resistance Gene Dissemination by Antioxidant-Producing Microorganisms in Activated Sludge Systems

Insight into the characteristics of microbial communities in a single-stage anammox reactor under different oxygen conditions

Removal of antibiotic resistance genes during livestock wastewater treatment processes: Review and prospects

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