Effects of methyl farnesoate on Krüppel homolog 1 (Kr-h1) during vitellogenesis in the Chinese mitten crab (Eriocheir sinensis)

Li, Xilei; Chen, Tiantian; Jiang, Hong; Huang, Jiawei; Huang, Mengting; Xu, Ruijuan; Xie, Qiming; Zhu, Hongzhen; Su, Shiping

doi:10.1016/j.anireprosci.2020.106653

Cited by 11 publications

(8 citation statements)

References 40 publications

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“…These findings are consistent with evidence that Kr-h1 is a JH responsive gene in B. terrestris [48,92]. Our gene knock-down experiments establish Kr-h1 as a pivotal signaling gene acting downstream of JH in bumble bees, consistent with its key roles in JH signaling pathways in other insect species [78,81,[86][87][88]90,[115][116][117][118][119][120].…”

Section: Discussionsupporting

confidence: 90%

Krüppel-Homologue 1 Mediates Hormonally-Regulated Dominance Rank in a Social Insect

Pandey¹,

Bloch²

2021

Preprint

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Dominance hierarchies are ubiquitous in invertebrates and vertebrates, but little is known on how genes influence dominance rank. Our gaps in knowledge are specifically significant concerning female hierarchies and in insects. To start filling these gaps we studied the social bumble bee Bombus terrestris, in which social hierarchies among females are common and functionally significant. Dominance rank in this bee is influenced by multiple factors, including juvenile hormone (JH) that is a major gonadotropin in this species. We tested the hypothesis that the JH responsive transcription factor Krüppel homologue 1 (Kr-h1) mediates hormonal influence on dominance behavior in the bumble bee. We first developed and validated a perfluorocarbon nanoparticles-based RNA interference protocol for knocking down Kr-h1 expression. We then used this procedure to show that Kr-h1 mediates the influence of JH not only on oogenesis and wax production, but also on aggression and dominance rank. To the best of our knowledge, this is the first study causally linking a gene to dominance rank in social insects, and one of only a few such studies in insects or in female hierarchies. These findings are important for determining whether there are general molecular principles governing dominance rank across gender and taxa.

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Section: Discussionsupporting

confidence: 90%

Krüppel-Homologue 1 Mediates Hormonally-Regulated Dominance Rank in a Social Insect

Pandey¹,

Bloch²

2021

Preprint

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“…These findings are consistent with evidence that Kr-h1 is a JH responsive gene in B. terrestris [47,83]. Our gene knock-down experiments establish Kr-h1 as a pivotal signaling gene that is expressed in multiple tissues and acts downstream of JH in bumble bees, consistent with its key roles in JH signaling pathways in other insect species [82,86,89,[94][95][96][97][132][133][134][135][136]. These findings also set the stage for a deeper study on JH signaling pathways and the genes with which Kr-h1 interacts in bumble bees, as well as other hymenopteran insects.…”

Section: Discussionsupporting

confidence: 89%

Krüppel-homologue 1 Mediates Hormonally Regulated Dominance Rank in a Social Bee

Pandey

Bloch

2021

Biology

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Dominance hierarchies are ubiquitous in invertebrates and vertebrates, but little is known on how genes influence dominance rank. Our gaps in knowledge are specifically significant concerning female hierarchies, particularly in insects. To start filling these gaps, we studied the social bumble bee Bombus terrestris, in which social hierarchies among females are common and functionally significant. Dominance rank in this bee is influenced by multiple factors, including juvenile hormone (JH) that is a major gonadotropin in this species. We tested the hypothesis that the JH responsive transcription factor Krüppel homologue 1 (Kr-h1) mediates hormonal influences on dominance behavior. We first developed and validated a perfluorocarbon nanoparticles-based RNA interference protocol for knocking down Kr-h1 expression. We then used this procedure to show that Kr-h1 mediates the influence of JH, not only on oogenesis and wax production, but also on aggression and dominance rank. To the best of our knowledge, this is the first study causally linking a gene to dominance rank in social insects, and one of only a few such studies on insects or on female hierarchies. These findings are important for determining whether there are general molecular principles governing dominance rank across gender and taxa.

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“…In D. melanogaster, the transcription factors Krüppel homolog 1 (Kr-h1), seance, ouija board, molting defective, ventral veins lacking, and Knirps are linked to Halloween gene expression (193)(194)(195). Kr-h1 is a critical component of the juvenile hormone (JH)/methyl farnesoate (MF) signaling pathway in insects (196)(197)(198), and recent studies indicate that Kr-h1 plays a role in crustacean development and reproduction (199)(200)(201)(202)(203). The YO expresses Kr-h1 and other MF signaling components, suggesting that it also has a role in molt regulation (204).…”

Section: Conclusion and Future Researchmentioning

confidence: 99%

Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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A pair of Y-organs (YOs) are the molting glands of decapod crustaceans. They synthesize and secrete steroid molting hormones (ecdysteroids) and their activity is controlled by external and internal signals. The YO transitions through four physiological states over the molt cycle, which are mediated by molt-inhibiting hormone (MIH; basal state), mechanistic Target of Rapamycin Complex 1 (mTORC1; activated state), Transforming Growth Factor-β (TGFβ)/Activin (committed state), and ecdysteroid (repressed state) signaling pathways. MIH, produced in the eyestalk X-organ/sinus gland complex, inhibits the synthesis of ecdysteroids. A model for MIH signaling is organized into a cAMP/Ca2+-dependent triggering phase and a nitric oxide/cGMP-dependent summation phase, which maintains the YO in the basal state during intermolt. A reduction in MIH release triggers YO activation, which requires mTORC1-dependent protein synthesis, followed by mTORC1-dependent gene expression. TGFβ/Activin signaling is required for YO commitment in mid-premolt. The YO transcriptome has 878 unique contigs assigned to 23 KEGG signaling pathways, 478 of which are differentially expressed over the molt cycle. Ninety-nine contigs encode G protein-coupled receptors (GPCRs), 65 of which bind a variety of neuropeptides and biogenic amines. Among these are putative receptors for MIH/crustacean hyperglycemic hormone neuropeptides, corazonin, relaxin, serotonin, octopamine, dopamine, allatostatins, Bursicon, ecdysis-triggering hormone (ETH), CCHamide, FMRFamide, and proctolin. Contigs encoding receptor tyrosine kinase insulin-like receptor, epidermal growth factor (EGF) receptor, and fibroblast growth factor (FGF) receptor and ligands EGF and FGF suggest that the YO is positively regulated by insulin-like peptides and growth factors. Future research should focus on the interactions of signaling pathways that integrate physiological status with environmental cues for molt control.

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Effects of methyl farnesoate on Krüppel homolog 1 (Kr-h1) during vitellogenesis in the Chinese mitten crab (Eriocheir sinensis)

Cited by 11 publications

References 40 publications

Krüppel-Homologue 1 Mediates Hormonally-Regulated Dominance Rank in a Social Insect

Krüppel-Homologue 1 Mediates Hormonally-Regulated Dominance Rank in a Social Insect

Krüppel-homologue 1 Mediates Hormonally Regulated Dominance Rank in a Social Bee

Signaling Pathways That Regulate the Crustacean Molting Gland

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