Effects of methanol, cyclohexane and methylene chloride extracts of Bidens pilosa on various gastric ulcer models in rats

Tan, Paul V.; Dimo, Théophile; Dongo, Étienne

doi:10.1016/s0378-8741(00)00290-7

Cited by 51 publications

(43 citation statements)

References 21 publications

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“…Its hypotensive effect on hypertensive induced rats (Dimo et al, 1998;Dimo et al, 2001; Dimo et al, 2003) and, its relaxant effect was also showed on isolated rat vascular smooth muscle (Nguelefack et al, 2005). Its antisecretory and antiulcerogenic properties on various gastric ulcers models in rat showed that the methylene chloride extract is the most potent inhibitor of gastric mucosal lesions caused by HCl/ethanol, and that the extract does not possess anti-secretory potential, and tended to increase gastric acidity when gastric outflow is obstructed by ligature; a possible cytoprotective action mediated by the cytoprotective action of endogenous prostaglandins was also demonstrated (Tan et al, 2000). Another team have tested its leaf extract on the snake venoms and found that Bidens pilosa leaf extract weakly antagonized the D. jamesoni venom and did not potentiate the SAV (Antivenon Serum) (Chippaux et al, 1997).…”

Section: Introductionmentioning

confidence: 93%

In vivo and in vitro effects of Bidens pilosa l. (asteraceae) leaf aqueous and ethanol extracts on primed-oestrogenized rat uterine muscle.

Longo

Rakotonirina

Rakotonirina³

et al. 2008

Afr. J. Trad. Compl. Alt. Med.

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Bidens pilosa L. is an Asteraceae growing in tropical zones, and traditionally utilized worldwide in herbal medicine. The present work is based on its traditional use during child birth as a labour facilitator. In vivo tests of acute toxicity showed a weak toxic effect for both extracts but the toxicity of the ethanol extract (LD 50 =6.15g/kg) was upper than that of the aqueous extract (LD 50 =12.30g/kg). The three-days uterotrophic assay on immature mice showed body weight gain followed by a concentration-dependent decrease up to 4mg/g and a concentration dependent uterine wet weight increase. The ethanol extract exhibited the higher body weight gain representing 22.8±0.7%, (P≤0.001), at the concentration of 500µg/g/day, while the aqueous extract was significantly more efficient on the uterine wet weight gain of 0.24±0.001% (P≤0.05), at the concentration of 1000µg/g/day. In vitro isometric contraction measurement of oestrogen-primed rat uterine strips showed a significant high aqueous extract-induced contractile effect from 0.03-1.97mg/ml: on the amplitude of contraction (EC 50 = 0.44±0.10mg/ml, P≤0.05), and on the rate (1.21±0.25mg/ml, P>0.05). Inspite of the higher effect of the aqueous extract on the tonus (57.23±23%), the ethanol extract showed a high effect (EC 50 = 0.34±0.09mg/ml, P≤0.05). The weak toxicity, the estrogenic-like and the oxytocic-like activities observed could explain the empirical use of Bidens pilosa leaf aqueous extract as an uterotonic preparation to enhance labour, probably due to the presence of biologically active compound(s) which act directly on the uterine muscle.

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Section: Introductionmentioning

confidence: 93%

In vivo and in vitro effects of Bidens pilosa l. (asteraceae) leaf aqueous and ethanol extracts on primed-oestrogenized rat uterine muscle.

Longo

Rakotonirina

Rakotonirina³

et al. 2008

Afr. J. Trad. Compl. Alt. Med.

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“…It can be thought that the cytoprotective effect of the extract results from the enhancement of the mucosal barrier through the increase production of prostanglandin. Yamamoto et al (1992) and Tan et al (2000) have shown the prostaglandin-like cytoprotective action of some hydroxychalcone and methylene chloride extract of Bidens pilosa respectively and the methanol leaf extract of A. africana might be producing its antiulcer effects through the same mechanism. The anti-ulcer effects of the methanol extract of the leaves of Aspilia africana may be due to the presence of triterpenes in the extract.…”

Section: Pylorus Ligated-induced Ulcermentioning

confidence: 99%

“…These results show that the methanolic extract of A. africana leaves has protective properties against HCl/ethanol induced ulcers in rats. This method of ulcer induction is being widely used and is a convenient way of assessing anti-ulcer activity of drug (Hara and Okabe, 1985, Tan et al, 1996, 2000. The gastric mucosal protection against irritant substances as HCl/ethanol can be mediated by a number of mechanisms that .41 Number of rats per group = 7 I = inhibition US = ulcerated surface *p< 0.05 statistically significant relative to control include enhanced gastric mucosal defence through increased mucus and/or bicarbonate production, reducing the volume of gastric acid secretion or by simply neutralizing the gastric acidity.…”

Section: Hcl/ethanol-induced Ulcermentioning

confidence: 99%

Short Communication: The antiulcer effects of the methanol extract of the leaves of Aspilia africana (Asteraceae) in rats

Nguelefack

Watcho

Wansi

et al. 2005

Afr. J. Trad. Compl. Alt. Med.

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Abstract.Aspilia africana (Asteraceae) is a plant currently used in Cameroon ethnomedicine for the treatment of stomach ailments. The methanol extract of the leaves of A. africana was investigated against gastric ulcerations induced by HCl/ethanol and pylorusligation. With both methods, the extract inhibited gastric ulcerations in a dose-related manner. Oral administration of the plant extract at the doses of 0.5 and 1 g/kg reduced gastric lesions induced by HCl/ethanol by 79 % and 97 % respectively. The extract at the dose of 1 g/kg reduced gastric lesion in the pylorus ligated rats by 52 % although the gastric acidity remained higher as compared to the control. These findings show that methanol extract of the leaves of A. africana possess potent antiulcer properties.

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“…Among them, the main compounds are flavonoids and polyacetylenes, which are major anti-inflammatory phytochemicals in Bp [21,22]. In addition, both androgen and flavonoids are polyphenolic heterocyclic compounds.…”

Section: Western Blotmentioning

confidence: 99%

The Effect of the Aqueous Extract of Bidens Pilosa L. on Androgen Deficiency Dry Eye in Rats

Zhang

Yang

et al. 2016

Cell Physiol Biochem

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Background/Aims: Bidens pilosa L. (Bp) is widely distributed in China and has been widely used as a traditional Chinese medicine. The aim of this study was to examine the effect of the extract of Bp on androgen deficiency dry eye and determine its possible mechanisms. Methods: Twenty-four rats were randomly divided into four groups: Group Con (control), Group Sal (physiological saline), Group Fin (oral finasteride), and Group Bp (oral finasteride and Bp). The dry eye model was established in group Fin and group Bp. Aqueous tear quantity was measured with phenol red-impregnated cotton threads with anesthesia. Tear film breakup time (BUT) and corneal epithelial damage were evaluated by fluorescein staining. Animals were sacrificed at 28 days, and ocular tissues (lacrimal gland and cornea) were evaluated with light microscopy; gene microarray analysis for inflammatory cytokines and Western blot were also performed. Results: Finasteride administration effectively induced dry eye in rats by 14 days after administration. Group Fin rats had significantly higher fluorescein staining scores and lower aqueous tear quantity and BUT than the group Con rats, and notable inflammatory cell infiltrates were observed in the lacrimal gland of group Fin rats. The fluorescein staining score, aqueous tear quantity and BUT significantly improved with Bp treatment in the group Bp rats, and the structures of the lacrimal gland were well maintained without significant lymphocyte infiltration. Cytokine antibody array data identified the cytokines B7-2/Cd86, IL-1β, IL-4, IL-6, IL-10, MMP-8, FasL, TNF-α and TIMP-1 as candidates for validation by Western blot. Expression levels of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, in group Fin were upregulated compared with group Con. Levels of anti-inflammatory cytokines, such as IL-4 and IL-10, in group Fin were also upregulated compared with those in group Con. Compared with group Fin, IL-1β, FasL, and TNF-α were significantly decreased in group Bp. Conclusion: The extract of Bp appears to be effective for the treatment of androgen deficiency dry eye in rats by improving aqueous tear quantity, maintaining tear film stability, and inhibiting the inflammation of the lacrimal gland.

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Effects of methanol, cyclohexane and methylene chloride extracts of Bidens pilosa on various gastric ulcer models in rats

Cited by 51 publications

References 21 publications

<i>In vivo</i> and <i>in vitro</i> effects of <i>Bidens pilosa</i> l. (asteraceae) leaf aqueous and ethanol extracts on primed-oestrogenized rat uterine muscle.

<i>In vivo</i> and <i>in vitro</i> effects of <i>Bidens pilosa</i> l. (asteraceae) leaf aqueous and ethanol extracts on primed-oestrogenized rat uterine muscle.

Short Communication: The antiulcer effects of the methanol extract of the leaves of <i>Aspilia africana</i> (Asteraceae) in rats

The Effect of the Aqueous Extract of Bidens Pilosa L. on Androgen Deficiency Dry Eye in Rats

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