Effects of metformin on adipose-derived stromal cell (ADSC) – Breast cancer cell lines interaction

Teufelsbauer, Maryana; Rath, Barbara H.; Plangger, Adelina; Staud, Clement; Nanobashvili, J.; Huk, Ihor; Neumayer, Christoph; Hamilton, Gerhard; Radtke, Christine

doi:10.1016/j.lfs.2020.118371

Cited by 12 publications

(14 citation statements)

References 43 publications

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“…All materials and methods were acquired respectively performed as described previously 14 . All methods were performed in accordance with the relevant guidelines and regulations.…”

Section: Methodsmentioning

confidence: 99%

“…Box chisels were fragmented and the resulting suspension incubated in RPMI-1640 medium (Seromed, Berlin, Germany) supplemented with 30% fetal bovine serum (Seromed) and antibiotics (Sigma-Aldrich, St. Louis, MO, USA). BM-MSCs that had attached to the tissue culture flasks were further cultivated and expanded 14 . The BM-MSCs were checked by flow cytometry for their expression of CD73, CD90, and CD105 and negative reactivity for CD34 using a Cytoflex Flow Cytometer (Beckman Coulter Germany GmbH, Krefeld, Germany).…”

Section: Isolation and Characterization Of Bm-mscsmentioning

confidence: 99%

“…The BM-MSCs were checked by flow cytometry for their expression of CD73, CD90, and CD105 and negative reactivity for CD34 using a Cytoflex Flow Cytometer (Beckman Coulter Germany GmbH, Krefeld, Germany). Antibodies and isotype controls were from Biolegend (San Diego, CA, USA) and secondary reagents from Sigma-Aldrich 14 . Data analysis and histogram overlays were done employing the Kaluza flow analysis software (Beckman Coulter).…”

Section: Isolation and Characterization Of Bm-mscsmentioning

confidence: 99%

“…The breast cancer cell lines MDA-MB-231, MDA-MB-436, T47D and HCC1937 were obtained from the ATCC (Rockville, MD, USA), cultured in RPMI-1640 medium and upon confluence, cells were detached with trypsin/EDTA (Sigma-Aldrich) and cells counted with a LUNA cell counter (Biozym, Vienna, Austria) 14 . The commercial ATCC cell lines MDA-MB-231, MDA-MB-436 and T47D have been established from pleural effusions and and HCC1937 from a local tumor.…”

Section: Breast Cancer Cell Linesmentioning

confidence: 99%

“…Experiments were done in duplicate. Arrays were evaluated using ImageJ and Origin 9.1 software (OriginLab, Northampton, MA, USA) 14 .…”

Section: Adipokine Western Blot Arraysmentioning

confidence: 99%

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Effects of Metformin on Bone-derived Mesenchymal Stromal Cell–breast Cancer Cell Line Interactions

Teufelsbauer

Lang

Plangger

et al. 2021

Preprint

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Metformin is used to treat patients with diabetes mellitus and that was found to lower the incidence of cancer. The present study investigated the effects of metformin on human bone-derived mesenchymal stromal cells (BM-MSC) and their breast cancer cell line interactions. BM-MSCs were tested for growth stimulation and migration controlling activity on four breast cancer cell lines employing MTT tests, migration scratch tests and assays of the expression of adipokines in Western Blot arrays. Compared to breast cancer cell lines, metformin significantly inhibited the proliferation of BM-MSC lines. Pretreatment of BM-MSCs with metformin showed variable effects on breast cancer cell lines depending on the specific BM-MSC cancer line combination. Metformin significantly impaired the migration of MDA-MB-231 and MDA-MB-436 in response to conditioned media (CM) of drug pretreated BM-MSCs. Metformin-induced alterations of adipokines by BM-MSC CM indicated increased osteogenic signaling and possibly impairment of metastasis. The anticancer activities of metformin seem to be the result of direct and indirect mechanisms. A lower metformin-induced protumor activity of BM-MSCs in the bone microenvironment seem to contribute to the anticancer effects of this drug in breast cancer patients.

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“…All materials and methods were acquired respectively performed as described previously 14 . All methods were performed in accordance with the relevant guidelines and regulations.…”

Section: Methodsmentioning

confidence: 99%

Section: Isolation and Characterization Of Bm-mscsmentioning

confidence: 99%

Section: Isolation and Characterization Of Bm-mscsmentioning

confidence: 99%

Section: Breast Cancer Cell Linesmentioning

confidence: 99%

“…Experiments were done in duplicate. Arrays were evaluated using ImageJ and Origin 9.1 software (OriginLab, Northampton, MA, USA) 14 .…”

Section: Adipokine Western Blot Arraysmentioning

confidence: 99%

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Effects of Metformin on Bone-derived Mesenchymal Stromal Cell–breast Cancer Cell Line Interactions

Teufelsbauer

Lang

Plangger

et al. 2021

Preprint

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The anti-aging role of metformin, clinical applications to pursue the longevity road

Pozzi,

Susini,

Di Seclì

et al. 2024

Eur J Plast Surg

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Effects of metformin on human bone-derived mesenchymal stromal cell—breast cancer cell line interactions

et al. 2022

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Metformin is used to treat patients with type 2 diabetes mellitus and was found to lower the incidence of cancer. Bone metastasis is a common impairment associated with advanced breast cancer. The present study investigated the effects of metformin on human bone-derived mesenchymal stromal cells (BM-MSC)—breast cancer cell line interactions. BM-MSCs grown from box chisels were tested for growth-stimulating and migration-controlling activity on four breast cancer cell lines either untreated or after pretreatment with metformin. Growth stimulation was tested in MTT tests and migration in scratch assays. Furthermore, the expression of adipokines of BM-MSCs in response to metformin was assessed using Western blot arrays. Compared to breast cancer cell lines (3.6 ± 1.4% reduction of proliferation), 500 µM metformin significantly inhibited the proliferation of BM-MSC lines (mean 12.3 ± 2.2 reduction). Pretreatment of BM-MSCs with metformin showed variable effects of the resulting conditioned media (CM) on breast cancer cell lines depending on the specific BM-MSC—cancer line combination. Metformin significantly reduced the migration of breast cancer cell lines MDA-MB-231 and MDA-MB-436 in response to CM of drug-pretreated BM-MSCs. Assessment of metformin-induced alterations in the expression of adipokines by BM-MSC CM indicated increased osteogenic signaling and possibly impairment of metastasis. In conclusion, the anticancer activities of metformin are the result of a range of direct and indirect mechanisms that lower tumor proliferation and progression. A lower metformin-induced protumor activity of BM-MSCs in the bone microenvironment seem to contribute to the positive effects of the drug in selected breast cancer patients.

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Effects of metformin on adipose-derived stromal cell (ADSC) – Breast cancer cell lines interaction

Cited by 12 publications

References 43 publications

Effects of Metformin on Bone-derived Mesenchymal Stromal Cell–breast Cancer Cell Line Interactions

Effects of Metformin on Bone-derived Mesenchymal Stromal Cell–breast Cancer Cell Line Interactions

The anti-aging role of metformin, clinical applications to pursue the longevity road

Effects of metformin on human bone-derived mesenchymal stromal cell—breast cancer cell line interactions

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