“…Box chisels were fragmented and the resulting suspension incubated in RPMI-1640 medium (Seromed, Berlin, Germany) supplemented with 30% fetal bovine serum (Seromed) and antibiotics (Sigma-Aldrich, St. Louis, MO, USA). BM-MSCs that had attached to the tissue culture flasks were further cultivated and expanded 14 . The BM-MSCs were checked by flow cytometry for their expression of CD73, CD90, and CD105 and negative reactivity for CD34 using a Cytoflex Flow Cytometer (Beckman Coulter Germany GmbH, Krefeld, Germany).…”