Effects of metals on feeding rate and digestive enzymes in Gammarus fossarum: An in situ experiment

Dedourge-Geffard, Odile; Palais, F.; Biagianti-Risbourg, S.; Geffard, Olivier; Geffard, Alain

doi:10.1016/j.chemosphere.2009.09.042

Cited by 110 publications

(56 citation statements)

References 57 publications

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“…Extra-and intracellular digestive processes are mediated by enzymes whose activity actually depends on their innate lytic properties, synthesis rate and secretion rate (extracellular enzymes only) (Yan et al 1996). These enzyme activity regulators are sensitive to interactions with chemical toxicants which may lead to a disruption of enzyme activities under chemical stress, as previously reported for several aquatic invertebrate species (Yan et al 1996;De Coen and Janssen 1997;Barfield et al 2001;Chen et al 2002;Le Bihan et al 2004;Li et al 2008;Dedourge-Geffard et al 2009;Kalman et al 2009;Boldina-Cosqueric et al 2010;Bourgeault et al 2010). Perturbation of digestive processes may affect individual energy balance and result in energy allocation disturbances with possible consequences on individual fitness.…”

Section: Introductionmentioning

confidence: 89%

“…Several authors have reported a depression of digestive carbohydrase activities in invertebrate species exposed in vitro or in situ to chemical pollutants, especially heavy metals (Farris et al 1994;Yan et al 1996;De Coen and Janssen 1997;Milam and Farris 1998;Barfield et al 2001;Chen et al 2002;Le Bihan et al 2004;Li et al 2008;Dedourge-Geffard et al 2009;Kalman et al 2009;Boldina-Cosqueric et al 2010;Bourgeault et al 2010). Digestive enzyme response to chemical stressors may actually be rather complex (Fig.…”

Section: Digestive Enzyme Responses: Integration Of Natural and Man-mmentioning

confidence: 95%

“…Resulting acid solutions, adjusted to 1.5 ml with deionized water, were processed for metal quantification by either flame (Zn) or flameless (Cu, Pb, Ni) atomic absorption spectrophotometry (flame AAS: Varian AA240FS; flameless AAS: Varian SpectrAA 220 Zeeman) as described in Dedourge-Geffard et al (2009). Body metal concentrations were expressed in lg of metal per g of dry weight (lg g -1 , dw).…”

Section: Body Metal Quantificationmentioning

confidence: 99%

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One-year monitoring of core biomarker and digestive enzyme responses in transplanted zebra mussels (Dreissena polymorpha)

et al. 2012

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A 12-month active biomonitoring study was performed in 2008-2009 on the Vesle river basin (Champagne-Ardenne, France) using the freshwater mussel Dreissena polymorpha as a sentinel species; allochthonous mussels originating from a reference site (Commercy) were exposed at four sites (Bouy, Sept-Saulx, Fismes, Ardre) within the Vesle river basin. Selected core biomarkers (acetylcholinesterase (AChE) activity, glutathione-S transferase (GST) activity, metallothionein concentration), along with digestive enzyme activities (amylase, endocellulase) and energy reserve concentrations (glycogen, lipids), were monitored throughout the study in exposed mussels. At the Fismes and Ardre sites (downstream basin), metallic and organic contamination levels were low but still high enough to elicit AChE and GST activity induction in exposed mussels (chemical stress); besides, chemical pollutants had no apparent deleterious effects on mussel condition. At the Bouy and Sept-Saulx sites (upstream basin), mussels obviously suffered from adverse food conditions which seriously impaired individual physiological state and survival (nutritional stress); food scarcity had however no apparent effects on core biomarker responses. Digestive enzyme activities responded to both chemical and nutritional stresses, the increase in energy outputs (general adaptation syndrome-downstream sites) or the decrease in energy inputs (food scarcity-upstream sites) leading to mid- or long-term induction of digestive carbohydrase activities in exposed mussels (energy optimizing strategy). Complex regulation patterns of these activities require nevertheless the use of a multi-marker approach to allow data interpretation. Besides, their sensitivity to natural confounding environmental factors remains to be precised.

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Section: Introductionmentioning

confidence: 89%

Section: Digestive Enzyme Responses: Integration Of Natural and Man-mmentioning

confidence: 95%

Section: Body Metal Quantificationmentioning

confidence: 99%

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One-year monitoring of core biomarker and digestive enzyme responses in transplanted zebra mussels (Dreissena polymorpha)

et al. 2012

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“…In situ bioassays were adapted from the method described in Dedourge- Geffard et al (2009). Three replicates of eight males per site were deployed for 3 weeks.…”

Section: Experiments 1: Vitellogenin Gene Expression Levels In Controlmentioning

confidence: 99%

Vitellogenin-like gene expression in freshwater amphipod Gammarus fossarum (Koch, 1835): functional characterization in females and potential for use as an endocrine disruption biomarker in males

Xuereb¹,

Bezin

Chaumot³

et al. 2011

Ecotoxicology

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The induction of vitellogenin (Vtg) synthesis is widely accepted as a biomarker of estrogenic exposure in male and juvenile fish. Vtg synthesis has emerged as an interesting endpoint to assess endocrine disruptor (ED) effects in crustaceans. However, studies reporting induction of Vtg in male crustaceans are lacking. This study investigated the expression of the Vtg gene in a freshwater amphipod, Gammarus fossarum, using calibrated real-time reverse transcription polymerase chain reaction (real-time RT PCR). First, we described the basal pattern of expression in healthy male and female organisms at different reproductive moult stages, in order to validate the function of this gene. Females expressed from 200 to 700 times more Vtg transcripts than males, depending on the female reproductive stage. Females displayed significant elevation of Vtg mRNA levels at the end of the inter-moult phase and at the beginning of the pre-moult phase. Second, male gammarids were exposed to the estrogenic compound nonylphenol (NP) (0.05, 0.5, 5 and 50 μg L(-1)) and to the anti-androgen cyproterone (1, 10, 100 and 1000 μg L(-1)) for 2, 4, 8 and 16 days. Both chemicals altered the pattern of interindividual variability of Vtg gene expression in males with strong induction in some individuals. Finally, the impact of urban wastewater treatment plants (WWTP) on male Vtg gene expression was assessed in organisms transplanted in the field during in situ bioassay campaigns in three different watersheds. Induction of the Vtg mRNA level was observed in males transplanted downstream from WWTP effluent discharge in two of the three study sites.

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“…Furthermore, efficient accumulations of metals, essential or not, has been reported in G. pulex or G. fassarum (Geffard et al 2010;Lebrun et al 2011). Because metal accumulations in gammarids can induce sublethal alterations at physiological, biochemical and behavioural levels, these amphipods are also suitable candidates for the development of exposure biomarkers (Dedourge-Geffard et al 2009;Felten et al 2008).…”

Section: Introductionmentioning

confidence: 98%

Modelling copper bioaccumulation in Gammarus pulex and alterations of digestive metabolism

Lebrun

Perret²,

Geffard

et al. 2012

Ecotoxicology

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Bioaccumulation enables to integrate the ability of aquatic organisms to regulate metals and effects of water chemistry on metal bioavailability. Linking this process to biological responses offers thus promising lines of enquiry for protecting aquatic ecosystems. This study aims at characterizing the mechanisms involved in waterborne Cu bioaccumulation and assessing metal impact on digestive metabolism in an ecosystem engineer widely distributed in Europe, Gammarus pulex. The organism was exposed to several Cu concentrations (from 0.5 to 100 μg/L) in aquatic microcosms to establish kinetic parameters for the construction and comparison of two bioaccumulation models, i.e. the biodynamic and saturation models. Cu uptake was recorded in waters exhibiting various concentrations of Na, Mg and Ca at environmental levels to assess the influence of cationic composition on bioaccumulation. Then, the effect of increasing Cu in exposure media on the digestive metabolism of G. pulex was investigated by measuring enzymatic activities (β-glucosidase, N-acetyl-β-glucosaminidase, β-galactosidase). We showed that the saturation model is more suitable than the biodynamic model to describe Cu bioaccumulation in gammarids due to a maximal capacity of animals to accumulate the metal. Cationic composition of water affected insignificantly Cu uptake. All activities of tested enzymes decreased with increasing Cu in exposure media but with different degrees. High correlations were established between the inhibition of enzymatic activities and amounts of Cu bioaccumulated by gammarids. These biological responses could thus provide early-warming of Cu impact on aquatic biota.

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Effects of metals on feeding rate and digestive enzymes in Gammarus fossarum: An in situ experiment

Cited by 110 publications

References 57 publications

One-year monitoring of core biomarker and digestive enzyme responses in transplanted zebra mussels (Dreissena polymorpha)

One-year monitoring of core biomarker and digestive enzyme responses in transplanted zebra mussels (Dreissena polymorpha)

Vitellogenin-like gene expression in freshwater amphipod Gammarus fossarum (Koch, 1835): functional characterization in females and potential for use as an endocrine disruption biomarker in males

Modelling copper bioaccumulation in Gammarus pulex and alterations of digestive metabolism

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