Effects of metals Cu, Fe, Ni, V, and Zn on rat lung epithelial cells

Riley, Mark R.

doi:10.1016/s0300-483x(03)00162-8

Cited by 86 publications

(61 citation statements)

References 27 publications

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“…This is somewhat unusual as the A549 cells usually respond with a slow decline in culture health with increasing concentration of a stressor. 17 The behavior observed here is related to the critical micelle concentration of Triton, which is approximately 0.26 mM.Is Below this, Triton causes only minimal damage; above this concentration Triton solubilizes portions of the cell membrane, leaving holes through which intracellular compounds may pass. This mechanism is supported by measurements of the release of LDH, normally an intracellular enzyme (Fig.…”

Section: Resultsmentioning

confidence: 75%

Evaluation of Toxic Agent Effects on Lung Cells by Fiber Evanescent Wave Spectroscopy

et al. 2005

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Biochemical changes in living cells are detected using a fiber probe system composed of a single chalcogenide fiber acting as both the sensor and transmission line for infrared optical signals. The signal is collected via evanescent wave absorption along the tapered sensing zone of the fiber. We spectroscopically monitored the effects of the surfactant Triton X-lOO, which serves as a toxic agent simulant on a transformed human lung carcinoma type II epithelial cell line (A549). We observe spectral changes between 2800-3000 cm-1 in four absorptions bands, which are assigned to hydrocarbon vibrations of methylene and methyl groups in membrane lipids. Comparison of fiber and transmission spectra shows that the present technique allows one to locally probe the cell plasma membrane in the lipid spectral region. These optical responses are correlated with cellular metabolic activity measurements and LDH (lactate dehydrogenase) release assays that indicate a loss of cellular function and membrane integrity as would be expected in response to the membrane solubilizing Triton. The spectroscopic technique shows a significantly greater detection resolution in time and concentration.

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Section: Resultsmentioning

confidence: 75%

Evaluation of Toxic Agent Effects on Lung Cells by Fiber Evanescent Wave Spectroscopy

et al. 2005

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“…Concentrations of PM below 2.5 μm in aerodynamic diameter (PM 2.5 ) are regulated in the United States to protect human health and there is mounting evidence that ultrafine particles (PM below 100 nm in aerodynamic diameter) are more hazardous to human health than larger particles (Oberdorster 2001). Though much work remains in identifying the components of PM responsible for health impacts, many heavy metals have been shown to be toxic (Lingard et al 2005;Riley et al 2003) and their emissions are regulated by the USEPA (National Emission Standards for Hazardous Air Pollutants) and by states (for example, California's Air Toxic Program). Trace elements, including most metals, released by high temperature anthropogenic sources are principally emitted in the submicrometer particle size range (Ondov and Wexler 1998).…”

Section: Introductionmentioning

confidence: 99%

A Novel Method Using Polyurethane Foam (PUF) Substrates to Determine Trace Element Concentrations in Size-Segregated Atmospheric Particulate Matter on Short Time Scales

Dillner

Shafer

Schauer

2007

Aerosol Science and Technology

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A PUF-ICP-MS method has been developed that can quantify a large number of elements in size-resolved ambient aerosol samples collected over short-time intervals (four hours in this study). The pre-cleaning and digestion protocols developed enabled the quantification of 29 elements (including Hg) in ambient PM sampled onto a PUF substrate. The microwave-assisted acid digestion method affects a total dissolution of both aerosol and substrate, producing a colorless solution with no trace of aerosol, PUF, or polypropylene particulates, and is compatible with many analytical techniques (e.g., ICP-MS, CVAF, GFAA). For the large majority of elements the PUF-ICP-MS method provides superior elemental detection capabilities than traditional XRF approaches and importantly, method detection limits (MDLs) for most studied elements are lower than achieved with a 4-hour MOUDI approach using optimized ICP-MS analysis. For example, MDLs for V, Sr, Cd, Sb, and Hg are respectively 0.15, 0.18, 0.034, 0.051, and 0.0055 (solvent cleaning only for Hg) ng m −3 . Only one-third, or less, of the PUF substrate is required for the ICP-MS analysis leaving the majority of the substrate available for additional characterization, e.g., organic speciation, of the same air mass. A demonstration study conducted in Phoenix, Arizona, shows that this new method can quantitatively resolve differences in element concentrations on short time scales and has the potential to be a powerful new tool for identifying sources of trace and ultra-trace elements. The highly size and time resolved data can also provide useful information for the study of health effects of individual or groups of elements.

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“…Además, puede participar en la reacción de Haber-Weiss, catalizando la formación de radicales hidroxilos a partir de peróxido de hidrógeno y anión superóxido 31,32 . Estos radicales hidroxilo generan infiltración de leucocitos, activación de NF-kB, que inducen liberación de citoquinas e inflamación de las vías aéreas, que explicaría el aumento de células totales en el LBA 7,33 .…”

Section: Discussionunclassified

“…La instilación intratraqueal de igual concentración de distintos metales (4,8 µg de metal en 0,1 mL de agua) a ratas Spargue-Dawley detectó 48 h post-instilación que zinc y cobre inducen aumento de macrófagos alveolares y de leucocitos polimorfonucleares y de la concentración de proteínas en el lavado broncoalveolar, mientras que otros metales como vanadio, níquel, hierro y plomo, no provocaron cambios significativos [5][6][7][8] .…”

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Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono

et al. 2017

Rev. méd. Chile

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Effect of copper sulphate on the lung damage induced by chronic intermittent exposure to ozone count, proteins, GGT and AP in BALF (p < 0.05). Foamy and pigmented alveolar macrophages were detected in all lungs of these animals (p < 0.001). Conclusions: Intratracheal instillation of a single dose of CuSO 4 in rats previously subjected to a chronic and intermittent exposure to ozone induces a neutrophil pulmonary inflammatory response and cytoplasmic damage in macrophages. (Rev Med Chile 2017; 145: 9-16)

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Effects of metals Cu, Fe, Ni, V, and Zn on rat lung epithelial cells

Cited by 86 publications

References 27 publications

Evaluation of Toxic Agent Effects on Lung Cells by Fiber Evanescent Wave Spectroscopy

Evaluation of Toxic Agent Effects on Lung Cells by Fiber Evanescent Wave Spectroscopy

A Novel Method Using Polyurethane Foam (PUF) Substrates to Determine Trace Element Concentrations in Size-Segregated Atmospheric Particulate Matter on Short Time Scales

Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono

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