Effects of mesenchymal stem cell transplantation on extracellular matrix after myocardial infarction in rats

Xu, Xiaohua; Xu, Zhen-Peng; Xu, Yuanyuan; Cui, Guanghui

doi:10.1097/00019501-200506000-00006

Cited by 62 publications

(37 citation statements)

References 26 publications

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“…In the present study, we did not find any detectable MMP-1 mRNA expression, which is consistent with the investigation by Li et al [29]. Additionally, we did not find any difference at the protein production level of MMP-1 among the three experimental groups at week nine post-MI, which is accordance with our previous study [30]. We speculate that MMP-1 expression is probably augmented temporarily after MI and returns to normal level thereafter.…”

Section: Discussionsupporting

confidence: 93%

Effects of exercise training on cardiac function and myocardial remodeling in post myocardial infarction rats

Xu¹,

Wan²,

Powers³

et al. 2008

Journal of Molecular and Cellular Cardiology

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Objective-To test the hypothesis that early exercise training after myocardial infarction (MI) could preserve cardiac function, alleviate left ventricular (LV) remodeling and induce a protective effect on morphology.Methods-Male Sprague-Dawley rats underwent coronary ligation or sham operation, and were assigned to 3 groups: Sham, sedentary MI (SedMI), and exercise MI (ExMI). We measured the changes in collagen volume fraction, matrix metalloproteinase (MMP) 1, tissue inhibitor matrix metalloproteinase 1 (TIMP-1), angiotensin II receptor type 1 (AT1), and angiotensin converting enzyme (ACE) at gene and protein levels after 8 weeks of exercise training. Cardiac functions were determined by echocardiographic and hemodynamic measurements.Results-Early exercise training after MI had no effect on LV wall thinning. Cardiac function was significantly preserved in the ExMI group in comparison to the SedMI group. The collagen volume fraction in the ExMI group was significantly lower than in the SedMI group. Compared to the SedMI group, the ExMI group showed a markedly decrease at both the gene and protein levels in TIMP-1 (P<0.05). No significant differences were found in MMP-1 among the three groups. MMP-1/TIMP-1 ratio in the ExMI group was significantly higher than in the SedMI group. In addition, the expression of AT1 protein in the ExMI group was significantly lower than in the SedMI group. Furthermore, both ACE mRNA expression and ACE binding in the ExMI group are significantly decreased compared to the SedMI group.Conclusions-Our results suggest that early exercise training after MI reduces TIMP-1 expression, improves the balance between MMPs and TIMPs, and mitigates the expressions of ACE and AT1 receptor. These improvements, in turn, attenuate myocardial fibrosis and preserve post-MI cardiac function.

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Section: Discussionsupporting

confidence: 93%

Effects of exercise training on cardiac function and myocardial remodeling in post myocardial infarction rats

Xu¹,

Wan²,

Powers³

et al. 2008

Journal of Molecular and Cellular Cardiology

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“…Conversely, direct injection of human stem cells-in particular, mesenchymal stem cells-into ischemic rat myocardium decreased fibrosis, and that resulted in the prevention of systolic and diastolic LV dysfunction without evidence of myocardial regeneration (52). Additionally, stem cell transplantation significantly attenuated the increase in cardiac expression of collagen types I and III, and TGF-␤ in the infarcted myocardium (53). Because stem cells express a number of molecules involved in the biogenesis of extracellular matrix such as adrenomedullin, thymosin-␤, thymocollagenase, MMPs, serine proteases, and their inhibitors, it has been suggested that transplanted stem cells can inhibit fibrosis through paracrine actions (54).…”

Section: Alterations Of Collagen Matrixmentioning

confidence: 99%

New Targets to Treat the Structural Remodeling of the Myocardium

González

Ravassa

Beaumont

et al. 2011

Journal of the American College of Cardiology

152

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Classical therapy of heart failure is based on treatment of its pre-disposing/triggering factors and of the neurohumoral activation secondary to the deterioration of cardiac function. A new view is emerging that proposes the direct intervention on the pathological structural remodeling of the myocardium as part of heart failure therapy. In fact, in conditions of chronic injury, the cardiomyocytic and the noncardiomyocytic components of the myocardium undergo a series of structural lesions (i.e., cardiomyocyte growth and death, inflammation, alterations of collagen matrix, and microvascular rarefaction) that are governed by a complex interplay of mechanisms. Our increasing knowledge of the role of these mechanisms in remodeling enables us not only to better understand how our more successful therapies work but also to explore novel therapies for the future. In this paper, we will examine recent insights from experimental and pilot clinical studies that have provided new targets for interventions to prevent or reverse inflammation, alterations of collagen matrix, and cardiomyocyte death.

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“…Transplanted MSCs reduce myofibroblasts through releasing MMPs (Almalki and Agrawal, 2016;Mias et al, 2009). In MI rat models, implanted MSCs reduce the expression of collagen types I and III, tissue inhibitor of metalloproteinase-1 (TIMP-1), MMP2, MMP9, and transforming growth factor-beta (TGF-beta) (Nagaya et al, 2005;Xu et al, 2005). It is interesting that transplanted MSC cardiomyocyte (MSC-CM) also express the ability to reduce the scarring process by downregulating fibroblast proliferation and inhibiting the expression of collagen type I and type III in myofibroblasts (Ohnishi et al, 2007a;Ohnishi et al, 2007b).…”

Section: Impact On Extracellular Matrix (Ecm) and Reduction Of Scar Fmentioning

confidence: 99%

The effects of transplanted cells in stem cell therapy for myocardial ischemia

Pham

2016

Biomed Res Ther

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It is known that myocardial infarction (MI) causes damages to the heart tissue and that present medical therapies, such as medication, stenting and coronary artery bypass surgery, cannot recover the injured heart. Fortunately, advances in stem cell research have brought hope of full heart recovery for myocardial ischemia patients. There have been many studies using cell therapies for myocardial ischemia, from preclinical trials to clinical trials. However, the biggest concern is the effect of transplanted cells in myocardial recovery. This review will focus on analyzing both the positive and negative effects of transplanted cells in myocardial recovery to better understand the underlying biological mechanisms and ways to evaluate safety and efficacy of cell transplantation in myocardial ischemia treatment.

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Effects of mesenchymal stem cell transplantation on extracellular matrix after myocardial infarction in rats

Abstract: Our results demonstrated that MSC transplantation could inhibit LV remodeling and improve heart function.

Cited by 62 publications

References 26 publications

Effects of exercise training on cardiac function and myocardial remodeling in post myocardial infarction rats

Effects of exercise training on cardiac function and myocardial remodeling in post myocardial infarction rats

New Targets to Treat the Structural Remodeling of the Myocardium

The effects of transplanted cells in stem cell therapy for myocardial ischemia

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