Effects of Melothria maderaspatana leaf extract on antioxidant status in sham-operated and uninephrectomized DOCA-salt hypertensive rats

Abstract

The present study was designed to investigate the antihypertensive and antioxidant effect of Melothria maderaspatana leaf extract (MME) on sham-operated and DOCA-salt (deoxycorticosterone acetate) induced hypertensive rats. Administration of DOCA-salt significantly increased the systolic (from 127 to 212 mm Hg) and diastolic (from 91 to 174 mm Hg) blood pressure compared to sham-operated control rats, while treatment with MME significantly reduced the systolic (from 212 to 135 mm Hg) and diastolic (from 174 to 96 mm Hg) blood pressure compared to hypertensive control. In DOCA-salt rats, the plasma and tissue concentration of thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxide (LOOH) significantly increased and administration of MME significantly reduced these parameters towards the levels in sham-operated control. In hypertensive rats, activities of the enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and levels of non-enzymatic antioxidants such as vitamin C, vitamin E and reduced glutathione (GSH) decreased significantly in the plasma and tissues. Administration of MME returned the enzymatic and non-enzymatic antioxidants towards sham-operated control. MME shows both antihypertensive and antioxidant properties in DOCA-salt hypertensive rats and, among the three different doses tested, 200 mg/kg caused the maximum effect.

Effect of morin, a flavonoid against DOCA-salt hypertensive rats: a dose dependent study

“…Histopathology of kidney also confirmed the biochemical findings of this study. The effect at a dose of 50 mg/kg bw of morin was more pronounced than that of the other two doses (25 and other Moraceae which are used as dietary agents and also as herbal medicines [18] . Morin exhibits many biological activities, including antioxidant [19,20] , anti-inflammatory [21] , antitumour [22] activities and possibly even protective effects against chronic diseases [23] .…”

“…Results: DOCAsalt hypertensive rats showed considerably increased systolic and diastolic blood pressure, serum hepatic marker enzyme activities such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma-glutamyl transpeptidase (GGT) and renal function markers (urea, uric acid and creatinine) in plasma. Oral administration of morin (25,50 and 75 mg/kg bw) brought back all the above parameters to near normal level. Histopathology of kidney also confirmed the biochemical findings of this study.…”

“…After uninephrectomy, UNX-rats were allowed to drink tap water ad libitum, with no further treatment, and DOCA-salt hypertensive rats were given 1% NaCl in the drinking water with weekly twice subcutaneous injection of DOCA [25 mg/ kg bw in 0.4 mL of DMF (vehicle) with mild heating] for six consecutive weeks [24,25] .…”

“…Group 1: UNX-rats; group 2: UNX-rats were treated with morin alone (75 mg/kg bw); group 3: DOCAsalt hypertensive rats; groups 4, 5 and 6 were hypertensive rats, which received different doses of morin 25,50 and 75 mg/kg bw.…”

“…Methods: Hypertension was induced in uninephrectomized rats by weekly twice subcutaneous injection of DOCA (25 mg/ kg bw) and 1% NaCl in the drinking water for six consecutive weeks. Effect of morin against DOCA-salt induced hypertension was evaluated by measuring blood pressure and performing biochemical estimations and histopathological examination of renal tissues.…”