Effects of melatonin treatment on follicular development and oocyte quality in Chios ewes — Short communication

Tsiligianni, Theodora; Valasi, I.; Cseh, Sándor; Vainas, E.; Faigl, V.; Samartzi, F.; Papanikolaou, T.; Dovolou, Eleni; Amiridis, G.S.

doi:10.1556/avet.57.2009.2.13

Cited by 20 publications

(8 citation statements)

References 17 publications

(21 reference statements)

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“…Additionally, melatonin promotes follicular development by increasing production of insulin-like growth factor I which stimulates mitogenic growth of cumulus cells [ 38 ]. Although previous in vivo studies demonstrated exogenous FSH may mask effects of melatonin on follicular development, exogenous melatonin tended to increase the number of developing follicles [ 39 ]. Perhaps the role of melatonin in regulation of germ cell development is species- or age-specific.…”

Section: Discussionmentioning

confidence: 99%

Melatonin-Mediated Development of Ovine Cumulus Cells, Perhaps by Regulation of DNA Methylation

et al. 2018

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Cumulus cells of pre-pubertal domestic animals are dysfunctional, perhaps due to age-specific epigenetic events. This study was designed to determine effects of melatonin treatment of donors on methylation modification of pre-pubertal cumulus cells. Cumulus cells from germinal vesicle stage cumulus oocyte complexes (COCs) were collected from eighteen lambs which were randomly divided into control group (C) and melatonin group given an 18 mg melatonin implant subcutaneous (M). Compared to the C group, the M group had higher concentrations of melatonin in plasma and follicular fluid (p < 0.05), greater superovulation, a higher proportion of fully expanded COCs, and a lower proportion of apoptotic cumulus cells (p < 0.05). Real-time PCR results showed that melatonin up-regulated expression of genes MT1, Bcl2, DNMT1, DNMT3a and DNMT3b, but down-regulated expression of genes p53, Caspase 3 and Bax (p < 0.05). Furthermore, melatonin increased FI of FITC (global methylation level) on cumulus cells (p < 0.05). To understand the regulation mechanism, the DNMTs promoter methylation sequence were analyzed. Compared to the C group, although there was less methylation at two CpG sites of DNMT1 (p < 0.05) and higher methylation at two CpG sites of DNMT3a (p < 0.05), there were no significant differences in methylation of the detected DNMT1 and DNMT3a promoter regions. However, there were lower methylation levels at five CpG sites of DNMT3b, which decreased methylation of detected DNMT3b promoter region on M group (p < 0.05). In conclusion, alterations of methylation regulated by melatonin may mediate development of cumulus cells in lambs.

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Section: Discussionmentioning

confidence: 99%

Melatonin-Mediated Development of Ovine Cumulus Cells, Perhaps by Regulation of DNA Methylation

et al. 2018

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“…Melatonin can be used alone, or combined with other hormonal treatments, after an artificial light (photoperiodic) treatment. In several studies concerning ewes of different breeds, melatonin administration has been found to advance the onset of reproductive activity (Arendt et al, 1983;Karsch et al, 1984;English et al, 1986); furthermore, melatonintreated ewes appeared to have better oocyte quality and higher ovulation and conception rates (Stellflug et al, 1988;Kouimtzis et al, 1989;Tsiligianni et al, 2009).…”

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confidence: 98%

Testicular function and semen characteristics of Awassi rams treated with melatonin out of the breeding season

Faigl

Keresztes

Kulcsár

et al. 2009

Acta Veterinaria Hungarica

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The objective of this study was to evaluate the effect of long-term melatonin treatment applied during the non-breeding season on semen characteristics, endocrine function of testicles and baseline level of insulin-like growth factor-I (IGF-I) in Awassi rams kept in the temperate continental zone of Europe and used as semen donors in an artificial insemination (AI) programme. On 23 February (day 0), slow-release melatonin implants were inserted subcutaneously into rams (n = 8). Control animals (n = 8) received no treatment. In both groups, basic semen parameters (concentration, total motility, fast and slow forward motility, morphology), GnRH-induced testosterone response and basal IGF-I concentration were evaluated on days 0, 47 and 71. No differences were found in concentration of spermatozoa, total motility, and numbers of spermatozoa with fast and slow progressive motility and normal/abnormal morphology between the melatonintreated and the control group. However, in melatonin-treated animals, basal and GnRH-induced testosterone levels were slightly elevated on day 47 and became significantly higher on day 71 (P < 0.05) as compared to controls. There was no difference in plasma IGF-I levels between the groups. In conclusion, slow-release melatonin applied during the non-breeding season improves testicular testosterone production but does not influence the semen characteristics and the IGF-I level of semen donor Awassi rams used in an AI programme and kept in the temperate continental zone of Europe.

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“…Its stimulate ovarian steroidogenic gene expression (95) and luteinisation of graffian follicle (96). During the anoestrous period, MLT regulate a follicular growth (have a strong role in regulation and development) and oocyte efficiency (97). Exogenous MLT conserve cumulus cells from DNA damage during In vitro maturation (IVM) (98) (99), and the MLT supplementation to the IVM and culture medium can reduced Reactive oxygen species (ROS) (antioxidant effect) and get better competence of oocytes, which led to rise the quality and quantity blastocyst proportions and improve the embryos quality.…”

Section: The Use Of Mlt In Reproductive Techniquesmentioning

confidence: 99%

The Role of Melatonin in Ewes Reproduction: a Review

2019

AJVS

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This review was assigned to illustrate the melatonin (MLT) effect on reproduction in sheep (ewes). However, the pathway by which MLT charge the seasonal reproduction are imperfectly understood in sheep, the researchers consents that MLT regulates reproduction under influence of day length (photoperiod) to guarantee that birth happen in appropriate date of the year, it’s called neuro-endocrine process. This mechanism mediates by the pineal hormone (MLT). The major role of MLT in ewes is to translate the photo signals into endocrine pulses (gonadotropin-releasing hormone (GnRH) secretion) at the end of the retino-pineal pathway. In sheep, the MLT receptors distributed in premammillary hypothalamus (PMH), pituitary gland and Suprachiasmatic nucleus (SCN), therefore, many brain loci are participating for MLT pathways to modify the seasonal reproduction. Melatonin stimulates GnRH secretion through effect in different regions and neurons in hypothalamus such as a pre-mammillary nucleus, Arcuate and several factors like kisspeptin, RF-amide related peptide-3 (RFRP-3) and Tyrosine Hydroxylase (TH). In addition, its indirectly control prolactin (PRL) output via an effect on Tuberalin release, which is mediate the mechanism of MLT activity on pituitary PRL secretion and regulate his seasonal cyclicity. The alter in day length is the principle ecological factor that control the breeding in seasonal domestic animals. Several reproductive activities are related to short days and begin during autumn when the day becomes short and a decline in temperature (short-day breeder). While expanding in the duration of light lead to a cessation in reproduction activities during late winter and early spring. In conclusion, according to the major physiological role of MLT, it can be used in different aspects in ewes reproduction industry such as induce oestrus, increment the ovulation rate and In vitro embryo production.

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Effects of melatonin treatment on follicular development and oocyte quality in Chios ewes — Short communication

Cited by 20 publications

References 17 publications

Melatonin-Mediated Development of Ovine Cumulus Cells, Perhaps by Regulation of DNA Methylation

Melatonin-Mediated Development of Ovine Cumulus Cells, Perhaps by Regulation of DNA Methylation

Testicular function and semen characteristics of Awassi rams treated with melatonin out of the breeding season

The Role of Melatonin in Ewes Reproduction: a Review

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