Effects of Melatonin on Cytokin Release and Healing of Colonic Anastomoses in an Experimental Sepsis Model

Abstract

BACKGROUND:The present objective was to identify effects of early melatonin application on healing of anastomotic wound and inflammation in an experimental sepsis model. METHODS: A total of 60 Wistar albino rats were divided into 2 groups. Cecal ligation puncture (CLP) and colonic resection anastomosis were performed on both the control group and the melatonin treatment group. Both groups were divided into 3 subgroups consisting of 10 rats each. One subgroup from each group underwent re-laparotomy at the 16 th hour, the next on the 3 rd day, and the final subgroup on the 7 th day. Presently evaluated were effects of melatonin treatment of early sepsis on interleukin-6 (IL-6), interleukin-10 (IL-10), interferon gamma (INF-γ), and C-reactive protein (CRP) levels, as well as burst pressures (BPs), collagen and hydroxyproline (OHP) content of the anastomotic segments, histopathologic healing, immunohistochemical expressions, CD34, and transforming growth factor beta (TGF-β). RESULTS: IL-6 and INF-γ levels of the treatment group showed a significant decrease at the 16 th hour and an increase on the 3 rd and 7 th postoperative days. IL-10 levels were significantly higher at the 16 th hour and significantly lower on the 3 rd and 7 th postoperative days in the control group (p<0.001 for each). The treatment group also showed significantly higher capillary permeability, fibroblast proliferation, and collagen deposits (p<0.001 for each). CD34 expression was significantly increased in the treatment group on the 7

Melatonin Supplementation, a Strategy to Prevent Neurological Diseases through Maintaining Integrity of Blood Brain Barrier in Old People

“…For example, melatonin improved cardiac mitochondria and survival rate in rat septic heart injury (Zhang et al, 2013) through inhibition of inducible nitric oxide synthase (iNOS) and preservation of neuronal nitric oxide synthase (nNOS; Ortiz et al, 2014) and attenuated sepsis-induced cardiac dysfunction via a PI3K/Akt-dependent mechanism (An et al, 2016). Furthermore, melatonin protected liver bioenergetics from sepsis-induced damage (Basile et al, 2004), modified cellular stress in the liver of septic mice by reducing ROS and increasing the unfolded protein response (Kleber et al, 2014), protected against sepsis-induced functional and biochemical changes in rat ileum and urinary bladder (Paskaloğlu et al, 2004), improved colonic anastomotic healing in a rat experimental sepsis model (Ersoy et al, 2016) and counteracted inducible mitochondrial nitric oxide synthase-dependent mitochondrial dysfunction in skeletal muscle (Escames et al, 2006) and diaphragm (López et al, 2006b) in septic mice.…”

Quercetin associated with dimethylsulfoxide has a curative effect on experimental colon anastomosis injury

“…As a matter of fact, there are studies that support the importance of antioxidant effect on healing of injuries [14][15][16] . Furthermore, it was also shown that antioxidant effect is very important in healing of injuries after colon surgeries [17][18][19][20] . When the beneficial effects of antioxidants on healing of injuries and the antioxidant effect of quercetin come together, it suggests that quercetin can contribute to the healing of colon anastomotic injury and can prevent the complications that occur after leakage.…”