Effects of melatonin feed on histology and antioxidant ability of the gills and oxygen consumption of Chinese mitten crab (Eriocheir sinensis), exposed to acute hypoxia stress

Yang, Xiaozhen; Shi, Xingliang; Wu, Mengyao; Pang, Yangyang; Song, Xiaozhe; Shi, Aoya; Niu, Chao; Cheng, Yongxu

doi:10.1016/j.aquaculture.2021.737015

Cited by 11 publications

(3 citation statements)

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“…Differences between the liver and the plasma may exist to cope with hypoxic stress. The decrease in T-AOC levels in this study suggests that hypoxia and reoxygenation processes reduce the antioxidant capacity of the liver, as T-AOC reflects the free radical scavenging activity throughout the body (Yang et al, 2021). Our results show that only subtle changes in SOD activity occurred or activity remained constant.…”

Section: Long-term Hypoxia and Reoxygenation Induces Oxidative Stressmentioning

confidence: 48%

Long-term hypoxia and reoxygenation induced oxidative stress lead to immunosuppression and apoptosis in golden pompano (Trachinotus blochii)

Sun

Yao

et al. 2023

Front. Mar. Sci.

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The fluctuations of dissolved oxygen (DO) often lead to hypoxia in aquaculture, which has a huge adverse impact on fish. This study mainly investigated the effects of long-term hypoxia on oxidative stress, immune response, and cell apoptosis in the liver of golden pompano (Trachinotus blochii), which is not tolerant to hypoxia. So we conducted a 14 day low oxygen stress experiment on the golden pompano with a DO of 3.0 ± 0.2 mg/L, then restore the DO to normal levels and continue the 14 day reoxygenation experiment. Results showed that hypoxia and reoxygenation led to significant changes in liver structure. During hypoxia and reoxygenation, the expression of oxidative stress related genes (SOD1, SOD2, GSH-Px, and CAT) and levels of antioxidant enzymes (CAT and MDA) in the liver were increased. Liver lysozyme activity and the relative expression of the pro-inflammatory factors interleukin (IL)-1β were significantly increased, but the expression of IL-34 was down-regulated during hypoxia. The expression of IL-12β was significantly increased during reoxygenation. The expression of anti-inflammatory factor IL-11 was decreased duringreoxygenation. The expression of toll like receptors (TLRs) -7, -8, and -9 increases after hypoxia and decreases after reoxygenation, indicating that both hypoxia and reoxygenation affect the immune response. In addition, during hypoxia and reoxygenation, TUNEL-positive signals increased, the bcl2/bax ratio decreased, the expression levels of caspases-3 and -8 were significantly up-regulated during hypoxia, and expression levels of caspases-9 was up-regulated during reoxygenation. In summary, hypoxia and reoxygenation can cause oxidative stress, induce inflammatory reactions, inhibit immune processes, activate apoptosis, and lead to liver damage of the golden pompano, which may be irreversible.

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Section: Long-term Hypoxia and Reoxygenation Induces Oxidative Stressmentioning

confidence: 48%

Long-term hypoxia and reoxygenation induced oxidative stress lead to immunosuppression and apoptosis in golden pompano (Trachinotus blochii)

Sun

Yao

et al. 2023

Front. Mar. Sci.

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“…Alternatively, fishes have also developed mechanism to ameliorate the magnitude of stress response by evoking recovery or ease response in order to regain the basal homeostatic status with the aid of neuroendocrine/cytogenic signals like melatonin and nitric oxide (Peter, 2013;Peter and Gayathry, 2021;Peter et al, 2022). Similarly, beneficial role of melatonin in lowering the magnitude of stress response has been proposed in many fishes (Sánchez-Vázquez et al, 2019;Yang et al, 2021a;Kruk et al, 2021). In addition, antistress and anti-depressant role of melatonin have been reported in higher vertebrates (Konakchieva et al, 1997;Paredes et al, 2007;Detanico et al, 2009;SajeebKhan and Peter, 2015) and melatonin has shown anti-hypoxic ability in Chinese mitten crab (Yang et al, 2021b).…”

Section: Introductionmentioning

confidence: 99%

Melatonin integrates multidimensional regulation of Na+/K+-ATPase in ionocytes and promotes stress and ease response in hypoxia-induced air-breathing fish: lessons from integrative approach

et al. 2023

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As circadian regulator, melatonin is involved in many physiological processes including ionosmotic regulation in fishes. Na+/K+-ATPase (NKA), an ubiquitous Na+/K+ transporter in ionocyte epithelia that drives electrochemical Na+ gradients and systemic osmotic integration, is a target of stress in fish. However, it is not certain how melatonin regulates NKA functions in ionocyte epithelia and how it modulates the adaptive response such as stress and ease response in fish particularly in hypoxia condition. We, thus, examined the short-term in vivo action of melatonin on the dynamics of NKA regulation in branchial, renal and intestinal ionocytes of hypoxia-induced air-breathing fish (Anabas testudineus Bloch). Interestingly, we found a rise in plasma melatonin in fish when kept for 30 min of forced submergence in water and that indicates a role for melatonin in hypoxia tolerance. A fall in blood [Na+, K+] occurred in these hypoxic fish which later showed a recovery after melatonin treatment. Similarly, melatonin favored the fall in NKA activity in branchial and renal epithelia of hypoxic fish, though it remarkably stimulated its activities in non-stressed fish. Likewise, melatonin that produced differential pattern of mRNA expression in nkaα1-subunit isoforms (nkaα1a, nkaα1b and nkaα1c) and melatonin receptor isoforms (mtnr1a, mtnr1bb, mtnr1bbx1x2) in the tested ionocyte epithelia, showed reversed expression in hypoxic fish. In addition, the rise in NKAα-protein abundance in branchial and renal epithelia of melatonin-treated hypoxic fish indicated a recovery action of melatonin. A higher NKAα-immunoreactivity was found in the immunohistochemical and immunofluorescent images of branchial ionocytes and renal proximal and distal ionocytes of hypoxic fish treated with melatonin. Furthermore, an activation of PKA and PKG-dependent phosphorylation was found in branchial epithelia of hypoxic fish. The generated integrative parabola model showed that melatonin has a maximum targeted action on NKA function in the renal epithelia, suggesting its lead role in the integration of ionosmotic balance during the recovery or ease response. Over all, the data indicate a multidimensional and preferential action of melatonin on NKA regulation in fish ionocytes that integrate the recovery action against hypoxia, thus pointing to a major role for melatonin in stress and ease response in this fish.

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“…recovery of the HIF-1α mRNA transcript level in the gills of C. sinensis probably because of its ability to adapt and tolerate hypoxic conditions.Diverse studies have indicated that hypoxic condition can promote physiological stress to most aerobic organisms, such as oxidative stress(Parrilla-Taylor & Zenteno-Savín, 2011;Zhao et al, 2020). Oxidative stress is characterized by increased production and accumulation of ROS, which can be scavenged by the antioxidant enzymes, for example SOD, CAT and GSH-PX(Yang et al, 2021;Zhao et al, 2020). Previous studies have indicated that some hypoxia-tolerant species exposed to hypoxia can motivate the antioxidant system increasing the defences and avoiding oxidative damage(Parrilla-Taylor & Zenteno-Savín, 2011;Zenteno- Savín et al, 2006).…”

mentioning

confidence: 99%

Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

Ni¹,

Wang²,

Xie³

et al. 2022

Aquaculture Research

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Aquatic hypoxia has negative or detrimental effects on clams. To cope with hypoxia stress, a complex series of physiological and biochemical strategies can be employed in clams. Hypoxia‐inducible factor‐1α (HIF‐1α) and antioxidant enzymes are commonly found in aquatic animals as an adaptation against hypoxia. In the present study, we cloned the HIF‐1α sequence in clam Cyclina sinensis, and it consists of 2654 base pairs (bp) carrying a single open‐reading frame that encompasses 2235 bp of the coding region. The predicted C. sinensis HIF‐1α protein (744 amino acids) has the conserved HLH‐PAS domain, which contained one N‐terminal basic helix–loop–helix (bHLH) domain and two Per‐ARNT‐Sim A/B (PAS) domains, followed by PAS‐associated C‐terminal (PAC) domain. HIF‐1α is expressed predominantly in gills. Exposed to hypoxia, the transcript level of HIF‐1α was significantly up‐regulated in response to hypoxia, and then, the transcript level declined continuously with the time elapsed. The antioxidant enzymes (SOD, CAT and GSH‐PX) in gills were increased initially and then decreased after acute hypoxia exposure. Our study indicates that the HIF‐1α gene and antioxidant enzymes may play an important role in cope with acute hypoxia exposure.

show abstract

Effects of melatonin feed on histology and antioxidant ability of the gills and oxygen consumption of Chinese mitten crab (Eriocheir sinensis), exposed to acute hypoxia stress

Cited by 11 publications

References 30 publications

Long-term hypoxia and reoxygenation induced oxidative stress lead to immunosuppression and apoptosis in golden pompano (Trachinotus blochii)

Long-term hypoxia and reoxygenation induced oxidative stress lead to immunosuppression and apoptosis in golden pompano (Trachinotus blochii)

Melatonin integrates multidimensional regulation of Na+/K+-ATPase in ionocytes and promotes stress and ease response in hypoxia-induced air-breathing fish: lessons from integrative approach

Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

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