Effects of Medium pH and Preconditioning Treatment on Protein Adsorption on 45S5 Bioactive Glass Surfaces

Höhn, Sarah; Zheng, Kai; Romeis, Stefan; Brehl, Martin; Ligny, Dominique de; Virtanen, Sannakaisa; Boccaccını, Aldo R.

doi:10.1002/admi.202000420

Cited by 14 publications

(10 citation statements)

References 64 publications

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“…Such transition is generally associated with the loss of molecular rigidity, which affects the N-terminal region and, thus, impacts ligand biding. 27 Finally, it was shown in ref ( 28 ) that preconditioning bioactive glass (45S5) at various pH levels can strongly influence the adsorption of BSA. The glass substrates were washed with the buffer solutions prior to applying the protein solution.…”

Section: Methodsmentioning

confidence: 99%

Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption

Hyunh

Palma

Rahikainen

et al. 2021

ACS Biomater. Sci. Eng.

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The traditional silicate bioactive glasses exhibit poor thermal processability, which inhibits fiber drawing or sintering into scaffolds. The composition of the silicate glasses has been modified to enable hot processing. However, the hot forming ability is generally at the expense of bioactivity. Metaphosphate glasses, on the other hand, possess excellent thermal processability, congruent dissolution, and a tailorable degradation rate. However, due to the layer-by-layer dissolution mechanism, cells do not attach to the material surface. Furthermore, the congruent dissolution leads to a low density of OH groups forming on the glass surface, limiting the adsorption of proteins. It is well regarded that the initial step of protein adsorption is critical as the cells interact with this protein layer, rather than the biomaterial itself. In this paper, we explore the possibility of improving protein adsorption on the surface of phosphate glasses through a variety of surface treatments, such as washing the glass surface in acidic (pH 5), neutral, and basic (pH 9) buffer solutions followed or not by a treatment with (3-aminopropyl)triethoxysilane (APTS). The impact of these surface treatments on the surface chemistry (contact angle, ζ-potential) and glass structure (FTIR) was assessed. In this manuscript, we demonstrate that understanding of the material surface chemistry enables to selectively improve the adsorption of albumin and fibronectin (used as model proteins). Furthermore, in this study, well-known silicate bioactive glasses (i.e., S53P4 and 13-93) were used as controls. While surface treatments clearly improved proteins adsorption on the surface of both silicate and phosphate glasses, it is of interest to note that protein adsorption on phosphate glasses was drastically improved to reach similar protein grafting ability to the silicate bioactive glasses. Overall, this study demonstrates that the limited cell/phosphate glass biological response can easily be overcome through deep understanding and control of the glass surface chemistry.

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Section: Methodsmentioning

confidence: 99%

Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption

Hyunh

Palma

Rahikainen

et al. 2021

ACS Biomater. Sci. Eng.

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“…For instance, acidic pH values have been shown to cause more protein adsorption amounts on BG surfaces. [204][205][206] A longer duration of exposure time increases the total protein quantity adsorbed. Furthermore, Figure 14.…”

Section: Mbg and Proteins Interactions: Protein Coronamentioning

confidence: 99%

Mesoporous Bioactive Glasses in Cancer Diagnosis and Therapy: Stimuli‐Responsive, Toxicity, Immunogenicity, and Clinical Translation

et al. 2021

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Cancer is one of the top life‐threatening dangers to the human survival, accounting for over 10 million deaths per year. Bioactive glasses have developed dramatically since their discovery 50 years ago, with applications that include therapeutics as well as diagnostics. A new system within the bioactive glass family, mesoporous bioactive glasses (MBGs), has evolved into a multifunctional platform, thanks to MBGs easy‐to‐functionalize nature and tailorable textural properties—surface area, pore size, and pore volume. Although MBGs have yet to meet their potential in tumor treatment and imaging in practice, recently research has shed light on the distinguished MBGs capabilities as promising theranostic systems for cancer imaging and therapy. This review presents research progress in the field of MBG applications in cancer diagnosis and therapy, including synthesis of MBGs, mechanistic overview of MBGs application in tumor diagnosis and drug monitoring, applications of MBGs in cancer therapy ( particularly, targeted delivery and stimuli‐responsive nanoplatforms), and immunological profile of MBG‐based nanodevices in reference to the development of novel cancer therapeutics.

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“…Given the similar particle size, surface charge, and SSA, the difference in protein adsorption on 100S, 99S, 95S, and 90S was assumed to be induced by their different CaO contents. BSA was selected as a model protein for this study given the abundant presence of albumin in serum proteins [28]. We first investigated the influence of BSA concentration on protein adsorption on BGNs.…”

Section: Effects Of Cao Content In Bgns On Protein Adsorptionmentioning

confidence: 99%

“…Interactions between BGs and proteins have been investigated to understand the protein adsorption kinetic, factors affecting protein adsorption, and the influence of adsorbed proteins on BG properties [15]. For example, we recently reported that protein adsorption on 45S5 BGs is affected by the medium pH [28]. Also, adsorbed serum proteins seem to be able to delay HA formation on BGs [35,36].…”

Section: Effects Of Cao Content In Bgns On Protein Adsorptionmentioning

confidence: 99%

“…Due to the relatively higher content of Ca in 90S, ion exchange, degradation and formation of an amorphous Ca-P phase could take place on 90S faster than that on 99S and 100S during their soaking in physiological fluids. These activities lead to a significant change in surface charge, composition, hydrophilicity, crystallinity, and topography on BGN surfaces [28,43,44]. All these changes together lead to the relatively low amount of BSA adsorption on 90S compared to other BGNs.…”

Section: Effects Of Cao Content In Bgns On Protein Adsorptionmentioning

confidence: 99%

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Protein Adsorption on SiO2-CaO Bioactive Glass Nanoparticles with Controllable Ca Content

Kapp

Xu³

et al. 2021

Nanomaterials

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Bioactive glass nanoparticles (BGNs) are emerging multifunctional building blocks for various biomedical applications. In this study, the primary aim was to develop monodispersed binary SiO2-CaO BGNs with controllable Ca content. We successfully synthesized such spherical BGNs (size ~110 nm) using a modified Stöber method. Our results showed that the incorporated Ca did not significantly affect particle size, specific surface area, and structure of BGNs. Concentrations of CaO in BGN compositions ranging from 0 to 10 mol% could be obtained without the gap between actual and nominal compositions. For this type of BGNs (specific surface area 30 m2/g), the maximum concentration of incorporated CaO appeared to be ~12 mol%. The influence of Ca content on protein adsorption was investigated using bovine serum albumin (BSA) and lysozyme as model proteins. The amount of adsorbed proteins increased over time at the early stage of adsorption (<2 h), regardless of glass composition and protein type. Further incubation of BGNs with protein-containing solutions seemed to induce a reduced amount of adsorbed proteins, which was more significant in BGNs with higher Ca content. The results indicate that the Ca content in BGNs is related to their protein adsorption behavior.

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Effects of Medium pH and Preconditioning Treatment on Protein Adsorption on 45S5 Bioactive Glass Surfaces

Cited by 14 publications

References 64 publications

Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption

Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption

Mesoporous Bioactive Glasses in Cancer Diagnosis and Therapy: Stimuli‐Responsive, Toxicity, Immunogenicity, and Clinical Translation

Protein Adsorption on SiO2-CaO Bioactive Glass Nanoparticles with Controllable Ca Content

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