Effects of media composition on submerged culture spores of the entomopathogenic fungus,Metarhizium anisopliaevar.acridumPart 2: Effects of media osmolality on cell wall characteristics, carbohydrate concentrations, drying stability, and pathogenicity

Leland, Jarrod E.; Mullins, Donald E.; Vaughan, Larry J.; Warren, H. L.

doi:10.1080/09583150400016910

Cited by 28 publications

(18 citation statements)

References 45 publications

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“…The culture medium was then filtered through 2 layers of cheese cloth (pore size ≈ 75 µm) and concentration of submerged conidia in the filtrate was determined using a haemacytometer (Petroff-Hausner). The morphology of submerged conidia was confirmed by using description from Jenkins and Prior (1993) and Leland et al (2005) as references. Each treatment had 4 replicates and repeated 4 times.…”

Section: Submerged Conidia Yield On 3 Different Mediummentioning

confidence: 94%

“…Furthermore, the former was cheaper and the resources to mass produce were more readily available compared to solid state fermentation. In addition some research showed that the alteration of medium can enhance the pathogenicity of submerged form of fungal culture (Leland et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Three different liquid media were derived from Jenkins and Prior (1993) (Jenkins medium); Leland et al (2005) (Leland medium);and Moslim et al (2009) (MPOB medium). Jenkins medium consists of 1% yeast extract and 1% sucrose.…”

Section: Preparation Of Liquid Medium For Fungal Mass Productionmentioning

confidence: 99%

“…Medium Carbon to Nitrogen ratio, carbohydrate sources, nitrogen sources, yeast extract and other nutrients have been reported to change fungal characteristic and pathogenicity (Mehta et al, 2012;Mustafa and Kaur, 2009;Jackson and Jaronski, 2009;Bharati et al, 2007;Rangel et al, 2006;Shah et al, 2005;Li and Holdom, 1995). The purpose of this study is to identify the suitable medium from three different sources derived from Moslim et al (2009), Leland et al (2005) and Jenkins and Prior (1993) to mass produce pathogenic submerged conidia for control of subterranean termite Coptotermes curvignathus and further use to formulate the biopesticide.…”

Section: Introductionmentioning

confidence: 99%

“…Robert and Sweeney (1982) reported that fungal submerged culture was less pathogenic compared to aerial conidia. However, Leland et al (2005) and Jenkins and Prior (1993) found that alternation on liquid medium could produce pathogenic submerged conidia. Medium Carbon to Nitrogen ratio, carbohydrate sources, nitrogen sources, yeast extract and other nutrients have been reported to change fungal characteristic and pathogenicity (Mehta et al, 2012;Mustafa and Kaur, 2009;Jackson and Jaronski, 2009;Bharati et al, 2007;Rangel et al, 2006;Shah et al, 2005;Li and Holdom, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Control of Subterranean Termite Coptotermes Curvignathus (Isoptera: Rhinotermitidae) by Entomopathogen Metarhizium Anisopliae Var. Anisopliae Cultured in Liquid State Fermentation

Nyam¹,

Bong²,

King³

2015

American Journal of Agricultural and Biological Sciences

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Solid state fermentation has been used for mass production of Metarhizium anisopliae many years ago. However, solid state fermentation was time consuming, costly and unable to produce in large amount. We examined the suitability of liquid state fermentation to mass produce M. anisopliae and the effect of culture medium on fungal pathogenicity. Three different liquid medium selected for this study was Jenkins medium, Leland medium and MPOB medium. The fungus was cultured for 5 days to examine the growth rate. Yield of submerged conidia in different medium was assessed on day 3. Fungal virulence was bioassayed on termite Coptotermes curvignathus. The results indicated that Jenkins medium yielded significantly lower growth rate and dry weight (1.472 mg mL −1 ), but the fungus exhibited highest virulence (100% mortality on day 4) among the 3 medium studied. MPOB medium had the highest growth rate and DW yield (1.961 mg mL −1 ) but achieved 100% mortality only on day 6. The LC 50 result showed Jenkins medium yielded the most virulent fungus culture compared to Leland medium and MPOB medium.

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Section: Submerged Conidia Yield On 3 Different Mediummentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Liquid Medium For Fungal Mass Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Control of Subterranean Termite Coptotermes Curvignathus (Isoptera: Rhinotermitidae) by Entomopathogen Metarhizium Anisopliae Var. Anisopliae Cultured in Liquid State Fermentation

Nyam¹,

Bong²,

King³

2015

American Journal of Agricultural and Biological Sciences

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Impact of osmotic stress on production, morphology, and fitness of Beauveria bassiana blastospores

Mascarin

Kobori

Coleman

et al. 2023

Appl Microbiol Biotechnol

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Endogenous arabitol and mannitol improve shelf life of encapsulated Metarhizium brunneum

Krell

Jakobs-Schoenwandt

Persicke

et al. 2018

World J Microbiol Biotechnol

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Successful commercialization of microbial biocontrol agents, such as Metarhizium spp., is often constrained by poor drying survival and shelf life. Here, we hypothesized that culture age would influence endogenous arabitol, erythritol, mannitol and trehalose contents in M. brunneum mycelium and that elevated levels of these compounds would improve drying survival and shelf life of encapsulated mycelium coupled with enhanced fungal virulence against T. molitor larvae. We found that culture age significantly influenced endogenous arabitol and mannitol contents in mycelium with highest concentrations of 0.6 ± 0.2 and 2.1 ± 0.2 µg/mg after 72 h, respectively. Drying survival of encapsulated mycelium was independent of culture age and polyol content with 41.1 ± 4.4 to 55.0 ± 6.2%. Best shelf life was determined for biomass harvested after 72 h at all investigated storage temperatures with maximum values of 59.5 ± 3.3% at 5 °C followed by 54.5 ± 1.6% at 18 °C and 19.4 ± 1.3% at 25 °C after 6 months. Finally, high fungal virulence against T. molitor larvae of 83.3 ± 7.6 to 98.0 ± 1.8% was maintained during storage of encapsulated mycelium for 12 months with larval mortalities being independent of culture age and polyol content. In conclusion, our findings indicate beneficial effects of endogenous polyols in improving shelf life of encapsulated mycelium and this may spur the successful development of microbial biocontrol agents in the future.

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Effects of media composition on submerged culture spores of the entomopathogenic fungus,Metarhizium anisopliaevar.acridumPart 2: Effects of media osmolality on cell wall characteristics, carbohydrate concentrations, drying stability, and pathogenicity

Cited by 28 publications

References 45 publications

Control of Subterranean Termite <i>Coptotermes Curvignathus</i> (Isoptera: Rhinotermitidae) by Entomopathogen <i>Metarhizium Anisopliae</i> Var. <i>Anisopliae</i> Cultured in Liquid State Fermentation

Control of Subterranean Termite <i>Coptotermes Curvignathus</i> (Isoptera: Rhinotermitidae) by Entomopathogen <i>Metarhizium Anisopliae</i> Var. <i>Anisopliae</i> Cultured in Liquid State Fermentation

Impact of osmotic stress on production, morphology, and fitness of Beauveria bassiana blastospores

Endogenous arabitol and mannitol improve shelf life of encapsulated Metarhizium brunneum

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