Effects of maternal vitamin D₃ during pregnancy on FASN and LIPE mRNA expression in offspring pigs

Abstract

In this study, sows were fed 200 (LD), 800 (ND) and 3200 (HD) IU of vitamin D3/kg basal diet during pregnancy (from 41 d to birth), respectively. All their offspring pigs were fed the same vitamin D3 replete die. At 150 days of age, a total of 18 offspring pigs (six offspring pigs per maternal diet group, sex balance) were weighed and slaughtered to investigate effects of maternal vitamin D3 during pregnancy on fatty acids synthase (FASN) and hormone-sensitive lipase (LIPE) expression in offspring pigs. The results showed that LD offspring pigs had higher FASN mRNA expression and the ratio of FASN/LIPE mRNA expression in subcutaneous adipose tissue, as well as higher LIPE mRNA expression of longissimus dorsal muscle, whereas, had lower the ratio of FASN/LIPE mRNA expression in longissimus dorsal muscle compared with ND or HD offspring pigs, respectively. Meanwhile, LD offspring pigs had higher carcass fat, average backfat thickness (ABFT), serum insulin and leptin levels, lower intramuscular fat (IMF), serum free fatty acid and triglycerol levels compared with ND or HD offspring pigs. In addition, the ratio of FASN/LIPE mRNA expression was negatively correlated with IMF content, and positively correlated to carcass fat content and ABFT in offspring pigs. Meanwhile, FASN mRNA expression was positively correlated with carcass fat content, while negatively correlated with ABFT in offspring pigs. These results suggested that maternal vitamin D3 affected fat accumulation and meat quality by regulating FASN and LIPE mRNA expression in offspring pigs.

Effects of maternal vitamin D₃ on quality and water distribution in pork of offspring pigs during frozen storage

“…It was previously demonstrated that shear force is negatively correlated to IMF content of muscle (Magnabosco et al, 2016). In this study, shear force in offspring pigs from LD group were significantly higher than that from HD group, which suggested that maternal vitamin D 3 deficiency may decreased the tenderness of longissimus dorsi muscle in offspring pigs during frozen storage through inhibiting the formation of IMF (Guo et al, 2020b). These results indicated that maternal vitamin D 3 status could affect the meat quality attributes in offspring pigs during frozen storage.…”

“…At day 150 of offspring age, longissimus dorsi muscle samples were collected within 24 h after slaughtering. Animal diets, feeding trait and slaughter methods are consistent with our previous study (Guo et al, 2020a;Guo et al, 2020b). Samples preparation of porcine longissimus dorsi muscles were according to previous reports (Zhang et al, 2019).…”

“…It was found that maternal vitamin D 3 status influenced adipocyte commitment and differentiation of musculus longissimus dorsi in piglets via changing adipogenic genes expression (Guo et al, 2020a). Meanwhile, maternal vitamin D 3 affected intramuscular fat (IMF) content and meat quality of offspring pigs by regulating fatty acid synthesis and hormone sensitive lipase mRNA expression (Guo et al, 2020b). In addition, vitamin D also has important direct effects on skeletal muscle (Abrams et al, 2018), and improving maternal vitamin D 3 status can promote prenatal skeletal muscle development in offspring pigs by regulating the expression of muscle transcription factors (Zhou et al, 2016).…”

A simple and green strategy for preparing poly(vinyl alcohol)/phosphate cellulose aerogel with enhanced flame‐retardant properties

“…Simultaneously, flame‐retardant PVA‐based materials have become a significant research hot spot because of its structure and performance advantages in aerogels. Montmorillonite (MMT), [ 19 ] clay, [ 20 ] hydroxyapatite, [ 21 ] and gelatin [ 22 ] are commonly used fillers for preparing flame retardant PVA aerogels. Chen's research group [ 23 ] reported four kinds of flame retardant PVA aerogels, specifically, PVA/SiO 2 , PVA/halloysite, PVA/MMT, and PVA/laponite.…”

Effects of maternal vitamin D3 status on quality characteristics of pork batters in offspring pigs during cold storage

“…In the lipid metabolism, vitamin D has potential nutritional regulation in animals (Wang et al, 2016). Maternal vitamin D 3 levels affected intramuscular adipocyte commitment and differentiation in piglets by regulating adipogenesis related genes expression (Guo et al, 2020a). Maternal vitamin D deficiency during pregnancy promoted the proliferation and differentiation of pre-adipocytes, and resulted in offspring obesity (Wen et al, 2018).…”