Effects of Maternal Protein-Calorie Malnutrition on the Phospholipid Composition of Surfactant Isolated from Fetal and Neonatal Rat Lungs. Compensation by Inositol and Lipid Supplementation

Guarner, Verónica; Tordet, C.; Bourbon, Jacques R.

doi:10.1203/00006450-199206000-00018

Cited by 22 publications

(11 citation statements)

References 16 publications

(29 reference statements)

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“…Guarner et al reported that phosphatidyl choline, a surfactant lipid component, is decreased in the fetal lungs derived from malnourished pregnant rats (15). The present study showed that the levels of SP-A in the fetal lungs and the amniotic fluid from protein-malnourished pregnant rats were significantly lower than those from preg nant rats receiving a normal protein diet.…”

Section: Discussionsupporting

confidence: 45%

“…However, there are few or no reports regarding the effects of nutritional states of pregnant mothers on surfactant protein levels in the fetal lungs. Malnutrition during pregnancy causes a delay in fetal lung maturation, and decreased respiratory functions in newborn infants (15,16). The purpose of the present study was to investigate the effects of protein malnutrition on the levels of SP-A in the fetal lungs and the amniotic fluid in pregnant rats.…”

mentioning

confidence: 97%

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Levels of Pulmonary Surfactant Protein A in Fetal Lung and Amniotic Fluid from Protein-malnourished Pregnant Rats.

Kohri

Sakai

Mizunuma

et al. 1996

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummarySurfactant protein A (SP-A) is a major apo-protein of pulmonary surfactant, which lines the alveolar walls, lowering the surface tension to prevent lung collapse. Pregnant rats were divided into two groups which received a diet with either 5% or 20% protein from gestational day 9. By a sensitive immunoassay, SP-A levels in the fetal lungs and the amniotic fluid showed a dramatic increase with advancing gestation after the initial appearance on gestational day 18 in both diet groups. Significantly lower levels of SP-A in pregnant rats fed 5% protein diet than those in pregnant rats fed 20% protein diet were observed in the fetal lungs on gestational day 21 and in the amniotic fluid on gestational days 20 and 21. The profiles of increased SP-A levels in the amniotic fluid reflected those in the fetal lungs during gestation. Immunohistochemical examination with anti-rat SP-A antibody at 21 days of gestation showed that the immunoreactive staining of bronchiolar epithelial Clara cells and alveolar type II cells were weaker in the fetal lung sections from pregnant rats fed 5% protein diet than in those from pregnant rats fed 20% protein diet. It is concluded that protein malnutrition in pregnant rats affects the biosynthesis of SP-A in the fetal lungs, which may have important consequences for prematurity and decreased respiratory functions in the neonatal lungs at birth.

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Section: Discussionsupporting

confidence: 45%

mentioning

confidence: 97%

Levels of Pulmonary Surfactant Protein A in Fetal Lung and Amniotic Fluid from Protein-malnourished Pregnant Rats.

Kohri

Sakai

Mizunuma

et al. 1996

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…Malnutrition can delay somatic growth and the development of new alveoli in addition to decreasing muscle strength, thereby prolonging the need for mechanical ventilation. In animal models, early malnutrition has been shown to alter surfactant lipid composition [11] .…”

mentioning

confidence: 99%

Nutrition and the Lung

Bhatia

Parish²

2009

Neonatology

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The use of surfactant replacement, ‘kinder gentler’ modes of ventilation combined with prenatal corticosteroids have all played a role in improved survival rates of very-low-birth-weight infants but have not reduced the prevalence of chronic lung disease. The increased rates of prematurity being observed in the United States along with the increased survival makes the overall problem of treatment of infants with established disease expensive spanning neonatology, pediatric critical care and general pediatrics and involving a myriad of specialists over the life of the surviving infant. However, none of the therapies used over the years have proven to be effective or have long-term adverse effects, nor have they been accepted as methods to prevent chronic lung disease. Although intuitively an appropriate nutritional therapy may be a useful adjunct in the care of the premature sick infant, perinatal malnutrition remains a major problem. The role of nutrition therapy in health and disease as it pertains to the lung will be reviewed.

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“…1999; 75(4):267-270: malnutrition, nicotine, lung phospholipids. descritos [1][2][3][4][5][6] , assim como também o impacto da desnutrição da rata grávida sobre seu feto [7][8][9][10] .…”

Section: Introductionunclassified

Effect of malnutrition and chronic use of nicotine during pregnancy on lung phospholipid concentration in neonate rats

Barbosa¹,

Kopelman²,

Amâncio³

et al. 1999

J Pediatr (Rio J)

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ResumoObjetivo: Avaliar se o uso crônico de nicotina, imposto à rata gestante, independentemente de seu estado nutricional, influencia a concentração de fosfolipídios pulmonares de seus recém-nascidos.Métodos: Oitenta ratas "Wistar EPM-1", após fecundação, e aleatoriamente, foram divididas em 4 grupos controles, que receberam ração normal e água ad libitum e sofreram injeção diária de nenhuma substância, 0,15 ml de solução fisiológica, 900 g/kg de bitartarato de nicotina a 95% e 2.700 g/kg de bitartarato de nicotina a 95%, respectivamente, e em 4 grupos desnutridas, que receberam 13g/dia de ração e água ad libitum e o mesmo tipo de tratamento. No 21º dia de gestação procedeu-se à cesariana, e oitenta recém-nascidos foram selecionados e divididos em 8 grupos de 10 cada, respeitando-se a procedência dos mesmos. Dois pares de pulmões foram extraídos, de cada grupo, para dosagem dos fosfolipídios pulmonares, realizada pelo método de Bartlett.Resultados: Observou-se aumento na concentração de fosfolipídios pulmonares dos recém-nascidos das ratas que não sofreram desnutrição e receberam nicotina, quando comparada com a dos outros grupos.Conclusões: O uso crônico de nicotina, imposto à rata gestante, aumenta a concentração dos fosfolipídios pulmonares nos seus recém-nascidos, e a desnutrição influencia esta concentração. AbstractObjective: The aim of the present study was to determine whether lung phospholipid concentration is affected in neonate rats "Wistar EPM-1" following a continuous 21-day gestational exposure to nicotine.Methods: Eighty rats "Wistar EPM-1" were randomly divided in four control (diet free and water "ad libitum") groups (10 rats each): 1 -Control, 2 -Physiologic Solution (infused with 0.15ml of NaCl 0.9%), 3 -Nicotine 1 (infused with 900 g/kg/day of nicotine bitartrate 95%), and 4 -Nicotine 2 (infused with 2.700 g/kg/day of nicotine bitartrate 95%), and four undernourished (diet 13g/day and water ad libitum) groups (10 rats each), that received the same kind of treatment as the control groups. The infusion of nicotine was subcutaneous. The offspring were divided in eight groups according to their origin.Results: A significant high lung phospholipid concentration was observed in the non-undernourished nicotized group which was injected with a high dose of nicotine. In the other groups, there was no alteration in that concentration.Conclusion: We conclude that gestational exposure to nicotine increases lung phospholipid concentration in neonate rats, and that the nutritional state also influences this lung phospholipid concentration. descritos [1][2][3][4][5][6] , assim como também o impacto da desnutrição da rata grávida sobre seu feto [7][8][9][10] .Diminuição na concentração de fosfolipídios surfactante tem sido verificada em seres humanos fumantes 11 , mas experimentalmente, em animais, no período gestacional, a ação da nicotina sobre a concentração dos fosfolipí-dios pulmonares de seus fetos 12,13 não está ainda bem esclarecida. Assim, este estudo teve o objetivo de avaliar IntroduçãoEfeitos a...

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Effects of Maternal Protein-Calorie Malnutrition on the Phospholipid Composition of Surfactant Isolated from Fetal and Neonatal Rat Lungs. Compensation by Inositol and Lipid Supplementation

Cited by 22 publications

References 16 publications

Levels of Pulmonary Surfactant Protein A in Fetal Lung and Amniotic Fluid from Protein-malnourished Pregnant Rats.

Levels of Pulmonary Surfactant Protein A in Fetal Lung and Amniotic Fluid from Protein-malnourished Pregnant Rats.

Nutrition and the Lung

Effect of malnutrition and chronic use of nicotine during pregnancy on lung phospholipid concentration in neonate rats

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