Effects of Marsdenia tenacissima polysaccharide on the immune regulation and tumor growth in H 22 tumor-bearing mice

Jiang, Shuang; Qiu, Limin; Li, Yiquan; Lü, Li; Wang, Xinyun; Li, Zhi; Guo, Yan; Wang, Haotian

doi:10.1016/j.carbpol.2015.10.056

Cited by 35 publications

(23 citation statements)

References 44 publications

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“…Previous studies have revealed that MT has capable anti-hepatoma property when used only or combined with a chemotherapeutic. One water-soluble polysaccharide of Marsdenia tenacissima polysaccharide can develop immune function in normal control mice and prevent the tumor formation in H22 hepatoma cells in tumor-induced mice by notably improved the activities of antioxidant substances such as SOD, GPx and CAT [15]. Thus, in the present study, we designed to synthesize and characterization of AuNPs with the remedial plant of MT and to investigate the anticarcinogenic potential of the synthesized Marsdenia tenacissima-gold nanoparticles (MT-AuNPs) against liver cancer HepG2 cell lines.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of gold nanoparticles from Marsdenia tenacissima and its anticancer activity of liver cancer HepG2 cells

Zhang²,

Seshadri

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Nowadays, the synthesis and characterization of gold nanoparticles (AuNPs) from plant based extracts and effects of their anticancer have concerned an important interest. Marsdenia tenacissima (MT), a conventional Chinese herbal medicine, has long been used for thousands of years to treat tracheitis, asthma, rheumatism, etc. In this present study, we optimize the reaction of parameters to manage the nanoparticle size, which was categorized by high-resolution transmission electron microscopy (HR-TEM). A different characterization method, for example, UV-visible spectroscopy (UV-vis), fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were performed to consider the synthesized AuNPs getting from the MT leaf extract. The MT-AuNPs were analyzed for their cytotoxicity property against HepG2 cells by MTT analysis. The apoptosis was evaluated by using reactive oxygen species (ROS), migration assay, mitochondrial membrane potential (MMP) and apoptotic protein expression. Interestingly, the findings of our study observed the cytotoxicity effect of synthesized MT-AuNPs at a concentration of 59.62 ± 4.37 lg after 24 hrs treatment. Apoptosis was induced by the MT-AuNPs with enhanced ROS, changed MMP and inhibit the migration assay. Finally, the apoptosis was confirmed by the considerable up-regulation of Bax, caspase-9 and caspase-3, while the anti-apoptotic protein expressions of Bcl-2 and Bcl-XL were down-regulated. Although, in this studies, we evaluated the characterization, synthesis and anticancer action of gold nanoparticles from MT (MT-AuNPS) helpful for liver cancer therapeutics. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of gold nanoparticles from Marsdenia tenacissima and its anticancer activity of liver cancer HepG2 cells

Zhang²,

Seshadri

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Previous studies have demonstrated that MTE is an effective treatment for various types of malignant cancer ( 10 – 21 ). However, the anti-cancer effects of MTE and the underlying mechanisms are not fully understood in HCC.…”

Section: Discussionmentioning

confidence: 99%

“…Clinical studies have demonstrated that administration of MTE represses hematologic neoplasm growth in tumor models of nude mice ( 17 ), and M. tenacissima polysaccharide dose-dependently inhibited the growth of hepatoma H22 tumor-bearing mice ( 21 ). In vitro studies have reported that MTE induces G0/G1 cell cycle arrest in human esophageal carcinoma cells and hematologic neoplasm cells ( 16 ).…”

Section: Discussionmentioning

confidence: 99%

“…A study by Huang et al ( 20 ) demonstrated that the anti-angiogenic effect of MTE is achieved by downregulation of vascular endothelial growth factor (VEGF)-A in human hepatoma cells (HepG2), as well as VEGF-A and VEGF receptor-2 in human umbilical vein endothelial cells ( 20 ). As for HCC, a study by Jiang et al ( 21 ) identified that the M. tenacissima polysaccharide was able to inhibit tumor growth in H22 tumor-bearing mice in a dose-dependent manner ( 21 ). However, limited attention has been directed towards the underlying mechanism by which MTE suppresses proliferation and promotes apoptosis in hepatoma carcinoma cells.…”

Section: Introductionmentioning

confidence: 99%

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Marsdeniae tenacissima extract-induced growth inhibition and apoptosis in hepatoma carcinoma cells is mediated through the p53/nuclear factor-κB signaling pathway

Wang

Ying

et al. 2017

Experimental and Therapeutic Medicine

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An extract from a traditional Chinese herb, Marsdeniae tenacissima (trade name, Xiao-Ai-Ping) has been approved for use on the Chinese market as a cancer chemotherapeutic agent for decades. Previous studies have demonstrated the cytostatic and pro-apoptotic effects of M. tenacissima extract (MTE) in multiple cancer cells. However, the contributions of MTE to the proliferation and apoptosis of hepatoma carcinoma cells and the underlying mechanisms remain unclear. In the present study, Bel-7402 cells were incubated with increasing concentrations of MTE ranging from 0–320 µl/ml to explore the effects and potential mechanisms of MTE on the proliferation and apoptosis of Bel-7402 cells. 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium, inner salt and propidium iodide (PI)-stained flow cytometry assays demonstrated that MTE significantly suppressed the proliferation of Bel-7402 cells in a dose-dependent manner by arresting the cell cycle at S phase (P<0.05). Annexin V-fluorescein isothiocyanate PI-stained flow cytometry confirmed the significantly pro-apoptotic effect of MTE at both 160 and 240 µl/ml (P<0.001). Reverse transcription-quantitative polymerase chain reaction and western blot analysis demonstrated that MTE (both 160 and 240 µl/ml) induced a significant downregulation of B-cell lymphoma (Bcl)-2 (P<0.01), upregulation of Bcl-2-associated X protein (P<0.01) and activation of caspase-3 (P<0.05). Furthermore, a significant downregulation of murine double minute-2 (MDM2) (P<0.001) and activation of p53 (P<0.001) in Bel-7402 cells following treatment with 160 or 240 µl/ml MTE was observed, accompanied by the inhibition of the nuclear factor (NF)-κB pathway (P<0.001). These results suggested that MTE inhibited growth and exhibited pro-apoptotic effects in Bel-7402 cells, which was mediated by downregulation of the MDM2-induced p53-dependent mitochondrial apoptosis pathway and blocking the NF-κB pathway. Overall, these data serve as preliminary identification of the significant roles of MTE in hepatic carcinoma cells, and suggest that MTE may be a promising candidate for hepatocellular carcinoma therapy.

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“…Traditional Chinese Medicines (TCMs) have a long history in the treatment of various cancers, and they were reported to have low cost and less side effects [6,7]. Senecio scandens Buch,-Ham.…”

Section: Introductionmentioning

confidence: 99%

Anti-tumor activity of polysaccharides extracted from <i>Senecio scandens</i> Buch, -Ham root on hepatocellular carcinoma

Dou¹,

Zhang²,

Han³

et al. 2017

Trop. J. Pharm Res

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Purpose: To optimize the extraction conditions of polysaccharides from the root of Senecio scandens Buch,-Ham. (PRS) and evaluate its anti-tumor effect on hepatocellular carcinoma. Methods: Response surface methodology (RSM) applied with a Box-Behnken design (BBD, three levels and three factors) was employed to determine the effect of extraction time, number of extraction and ratio of water to raw material on the yield of PRS. The anti-tumor effect of PRS on A549, HL60, S180 and H22 cell lines was evaluated in vitro by 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay, while in vivo anti-tumor effect was evaluated in 20, 40 and 80 mg/kg (p < 0.05, p < 0.05 and p < 0.01, respectively). Treatment with PRS (20, 40 and 80 μg/mL)

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Effects of Marsdenia tenacissima polysaccharide on the immune regulation and tumor growth in H 22 tumor-bearing mice

Cited by 35 publications

References 44 publications

Synthesis and characterization of gold nanoparticles from Marsdenia tenacissima and its anticancer activity of liver cancer HepG2 cells

Synthesis and characterization of gold nanoparticles from Marsdenia tenacissima and its anticancer activity of liver cancer HepG2 cells

Marsdeniae tenacissima extract-induced growth inhibition and apoptosis in hepatoma carcinoma cells is mediated through the p53/nuclear factor-κB signaling pathway

Anti-tumor activity of polysaccharides extracted from <i>Senecio scandens</i> Buch, -Ham root on hepatocellular carcinoma

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