Effects of mammalian insulin on blood glucose level, glucose tolerance, and glycogen content of musculature and hepatopancreas in a gastropod mollusk, Strophocheilus oblongus

Marques, M. G.; Falkmer, Sture

doi:10.1016/0016-6480(76)90036-8

Cited by 32 publications

(5 citation statements)

References 24 publications

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“…Vertebrate (bovine) insulin did not decrease hemolymph glucose in Aplysia in the present study, although vertebrate insulin may have some effect in other molluscs (Marques & Falkmer, 1976; Rajan & Sriramulu, 1978). However, a putative ILF obtained from a nerve homogenate reliably decreased hemolymph glucose.…”

Section: Discussioncontrasting

confidence: 56%

“…Although injection of vertebrate insulin has been reported to affect hemolymph glucose in other gastropod molluscs (Marques & Falkmer, 1976; Rajan & Sriramulu, 1978), vertebrate insulin was not found to significantly decrease hemolymph glucose in Aplysia ( n = 4 repeated measures, 100 U/kg insulin vs. ASW). However, injections of Aplysia ILF significantly decreased hemolymph glucose at 3 hr compared with control nerve homogenate injection ( p s < .05 versus controls;Figures 8 a and 8b).…”

Section: Methods and Resultsmentioning

confidence: 97%

“…Experiments in which hemolymph glucose was sampled at different times of day suggest that the fall in hemolymph glucose is probably not due to a circadian rhythm. It is also unlikely that this fall in hemolymph glucose is due to a stress response, because stress stimuli (such as handling or electric shock, applied to gastropod molluscs) produce a rise in hemolymph glucose (Marques & Falkmer, 1976; Ram & Young, 1992) rather than a decrease. It is possible that the fall in hemolymph glucose is related to tissue damage produced by the syringe needle used for sampling.…”

Section: Discussionmentioning

confidence: 99%

Section: -3 3-6 6-24 Time After Injection (Hr)mentioning

confidence: 99%

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Evidence that hemolymph glucose in Aplysia californica is regulated but does not affect feeding behavior.

Horn¹,

Koester²,

Kupfermann³

1998

Behavioral Neuroscience

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Hemolymph glucose increased following a meal of a commercially available dried seaweed (laver) in Aplysia californica (Aplysia). Glucose injected into the hemocoel did not affect meal size, bite latencies, swallowing rate, or 24-hr food intake. The authors found that injection of a homogenate of nerves containing a putative Aplysia insulin-like substance decreased hemolymph glucose. The nerve homogenate, however, did not affect feeding behavior. Injection of 2-deoxy-D-glucose was found to increase hemolymph glucose, an indication of gluco-privation, but instead of increasing feeding it either had no effect or, at high doses, debilitated animals and interfered with feeding. These studies suggest that glucose may be physiologically regulated in Aplysia, but it does not appear to play a role in the control of feeding behavior.

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Section: Discussioncontrasting

confidence: 56%

Section: Methods and Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: -3 3-6 6-24 Time After Injection (Hr)mentioning

confidence: 99%

See 2 more Smart Citations

Evidence that hemolymph glucose in Aplysia californica is regulated but does not affect feeding behavior.

Horn¹,

Koester²,

Kupfermann³

1998

Behavioral Neuroscience

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“…Regardless of the strategy employed, all anoxia‐tolerant animals accumulate high levels of glycogen, the main fermentable fuel found in animal cells (Urich, '94; Lutz and Storey, '97; Hochachka and Somero, 2002). The total glycogen level found in M. abbreviatus CNS was high (De Fraga et al, 2004) as compared with the other snail tissues, including heart, mantle, and hepatopancreas (Haeser and De Jorge, '71; Marques and Falkmer, '76; Rossi and Da Silva, '93; Da Silva and Zancan, '94; Dias, 2000).…”

Section: Discussionmentioning

confidence: 99%

Control of carbohydrate metabolism in an anoxia‐tolerant nervous system

2010

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Anoxia-tolerant animal models are crucial to understand protective mechanisms during low oxygen excursions. As glycogen is the main fermentable fuel supporting energy production during oxygen tension reduction, understanding glycogen metabolism can provide important insights about processes involved in anoxia survival. In this report we studied carbohydrate metabolism regulation in the central nervous system (CNS) of an anoxia-tolerant land snail during experimental anoxia exposure and subsequent reoxygenation. Glucose uptake, glycogen synthesis from glucose, and the key enzymes of glycogen metabolism, glycogen synthase (GS) and glycogen phosphorylase (GP), were analyzed. When exposed to anoxia, the nervous ganglia of the snail achieved a sustained glucose uptake and glycogen synthesis levels, which seems important to maintain neural homeostasis. However, the activities of GS and GP were reduced, indicating a possible metabolic depression in the CNS. During the aerobic recovery period, the enzyme activities returned to basal values. The possible strategies used by Megalobulimus abbreviatus CNS to survive anoxia are discussed.

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Development of Drosophila imaginal discs in vitro: Effects of ecdysone concentration and insulin

Martin

Shearn

1980

J. Exp. Zool.

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A detailed scoring system was used to determine the effects of a variety of factors on the evagination and differentiation of Drosophila imaginal discs cultured in vitro. In modified Schneider's Drosophila medium, P-ecdysone induced development a t concentrations approximately 1 0 0~ lower than aecdysone. The metamorphosis of imaginal discs consists of two processes, evagination and differentiation, which were found to be differentially sensitive to hormone concentration. Optimal differentiation occurred at relatively low P-ecdysone concentrations (30 ngiml), while complete evagination required higher levels (500 ng/ml). Even at the optimal hormone concentration for differentiation, not all discs differentiated completely, and each region of the eye, antenna, wing, and leg exhibited a characteristic frequency of differentiation. In addition, a strong negative correlation between the extent of evagination and the quality of differentiation was observed. Bovine insulin stimulated P-ecdysone induced evagination, but at the same time it inhibited differentiation.The imaginal discs of Drosophila melanogaster can be maintained in vitro, and, if the culture medium is supplemented with a molting hormone, will undergo metamorphic development and differentiate the complete range of adult cuticular structures. This culture system promises to be a useful tool in the investigation of developmental processes of imaginal discs.

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Effects of mammalian insulin on blood glucose level, glucose tolerance, and glycogen content of musculature and hepatopancreas in a gastropod mollusk, Strophocheilus oblongus

Cited by 32 publications

References 24 publications

Evidence that hemolymph glucose in Aplysia californica is regulated but does not affect feeding behavior.

Evidence that hemolymph glucose in Aplysia californica is regulated but does not affect feeding behavior.

Control of carbohydrate metabolism in an anoxia‐tolerant nervous system

Development of Drosophila imaginal discs in vitro: Effects of ecdysone concentration and insulin

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