Effects of magnesium picolinate, zinc picolinate, and selenomethionine co-supplementation on reproductive hormones, and glucose and lipid metabolism-related protein expressions in male rats fed a high-fat diet

Şahin, Kazım; Orhan, Cemal; Küçük, Osman; Tuzcu, Mehmet; Şahin, Nurhan; Özercan, İbrahim; Sylla, Sarah; Ojalvo, Sara Perez; Komorowski, James

doi:10.1016/j.fochms.2022.100081

Cited by 2 publications

(2 citation statements)

References 55 publications

(76 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Levels of inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in the skin wound and burn tissues were determined by Western blot analysis, as described ( 26 ). The insulin resistance index, was calculated by homeostasis model assessment of insulin resistance (HOMA-IR), as previously published ( 32 ). The HOMA-IR score was calculated as the product of the fasting insulin level (mU/L) and the fasting glucose level (mmol/L), divided by 76.4 for rats.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

RJX Improves Wound Healing in Diabetic Rats

Uckun¹,

Orhan

Tuzcu

et al. 2022

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

BackgroundWe recently reported the clinical safety profile of RJX, a well-defined intravenous GMP-grade pharmaceutical formulation of anti-oxidant and anti-inflammatory vitamins as active ingredients, in a Phase 1 study in healthy volunteers (ClinicalTrials.gov Identifier: NCT03680105) (Uckun et al., Front. Pharmacol. 11, 594321. 10.3389/fphar.2020.594321). The primary objective of the present study was to examine the effects of GMP-grade RJX on wound and burn injury healing in diabetic rats.MethodsIn the present study, a rat model of T2DM was used that employs HFD in combination with a single injection of STZ intraperitoneally (i.p) at a moderate dose level (45 mg/kg). Anesthetized diabetic rats underwent full-thickness skin excision on the back or were subjected to burn injury via a heated brass probe and then started on treatments with normal saline (NS = vehicle) or RJX administered via intraperitoneal injections for three weeks.FindingsNotably, diabetic rats treated with the 1.25 mL/kg or 2.5 mL/kg RJX (DM+RJX groups) rapidly healed their wounds as fast as non-diabetic control rats. Inflammatory cell infiltration in the dermis along with fibrin and cell debris on the epithelial layer persisted for up to 14 days in the DM+NS group but not in RJX-treated groups. The histopathological score of wound healing on days 7 and 14 was better in diabetic rats treated with RJX than diabetic rats treated with NS and comparable to the scores for non-diabetic healthy rats consistent with an accelerated healing process. The residual wound area of RJX-treated rats was significantly smaller than that of NS-treated diabetic rats at each evaluation time point (P<0.001). The accelerating effect of RJX on diabetic wound healing was dose-dependent. We obtained similar results in the burn injury model. Our results demonstrate that RJX – at a dose level >10-fold lower than its clinical maximum tolerated dose (MTD) – accelerates the healing of excision wounds as well burn injury in diabetic rats.

show abstract

Section: Methodsmentioning

confidence: 99%

“…We used a HOMA-IR cut-off value of 2.5 to define insulin resistance. Rats presenting with HOMA-IR ≥ 2.50 were considered insulin-resistant ( 32 ). Skin tissue hydroxyproline and total protein measurements were performed, as previously published ( 33 ).…”

Section: Methodsmentioning

confidence: 99%