Effects of magnesium chloride on in vitro cholinesterase and ATPase poisoning by organophosphate (chlorpyrifos)

Abstract

The present study investigated possible benefits of magnesium ion (as MgCl2) in organophosphorus poisoning targeting its ability to interact with substrates and membrane enzymes. Blood samples collected from volunteered healthy adult by venepuncture into anticoagulant test tubes containing EDTA were separated into plasma and red blood cell and divided into three groups namely: normal, pesticide only (0.25‐2.0 mmol/L chlorpyrifos) and pesticide (0.25‐2.0 mmol/L chlorpyrifos) + 0.1 mol/L MgCl2. Acetylcholinesterase, Na+/K+ ATPase and Ca2+ ATPase activities were evaluated. Results showed that Chlorpyrifos significantly (P < .05) reduced the levels of cholinesterase both in plasma and on red blood cells. Red blood cells Na+/K+ ATPase and Ca2+ ATPase were also significantly (P < .05) reduced by chlorpyrifos while MgCl2 counteracted effects of chlorpyrifos with significant (P < .05) increase in the levels of cholinesterase, Na+/K+ ATPase and Ca2+ ATPase. We concluded that MgCl2 neutralized effects of chlorpyrifos by promoting normal ATPase activities and inhibiting release of acetylcholine from cell.

Chlorpyrifos Suppresses Neutrophil Extracellular Traps in Carp by Promoting Necroptosis and Inhibiting Respiratory Burst Caused by the PKC/MAPK Pathway

“…Then, the cells were suspended in modified RPMI medium (HyClone, Logan, UT, USA) containing 10% fetal bovine serum (NQBB, Australia) and seeded in 6-well plates (Corning, China). The cells were treated with various concentrations of CPF (20 mg/L, 15 mg/L, 3 mg/L, 1.5 mg/L, 0.75 mg/L, 0.325 mg/L, and 0.1625 mg/L) [27] for 2 h at 25°C in a humidified atmosphere containing 95% air and 5% CO 2 . To determine a suitable working concentration of CPF, cell viability was determined by a Cell Counting Kit-8 (MedChemExpress, Monmouth Junction, NJ, USA) according to the manufacturer's protocols.…”

Physiology of magnesium metabolism and the use of magnesium in intensive care (part 2)

“…Активность Na + / K + -АТФазы и Са 2+ -АТФазы эритроцитов также была значительно (P < 0,05) снижена хлорпирифосом, в то время как магния хлорид противодействовал эффектам хлорпирифоса со значительным (P < 0,05) повышением активности холинэстеразы, Na + /K + -ATФaзы и Ca 2+ -ATФaзы. Ученые пришли к выводу, что магния хлорид нейтрализует эффекты хлорпирифоса, способствуя нормальной активности АТФазы и ингибируя высвобождение ацетилхолина из клетки [68].…”

Магнийсодержащие Лекарственные Препараты: От Клинической Фармакологии До Фармацевтической Помощи (2-Я Часть)

“…Улучшает качество жизни пациентов с фибромиалгией (при применении трансдермальных лекарственных форм) [15]. Нейтрализует действие хлорпирифоса, способствуя нормальной активности АТФазы и ингибируя высвобождение ацетилхолина из клетки [16]. Обладает антимикробной активностью, в т. ч. при лечении кожных заболеваний [17,18].…”

“…It improves the quality of life of patients with fibromyalgia (when using transdermal dosage forms) [15]; It neutralizes the action of chlorpyrifos, promotes normal ATPase activity and inhibits the release of acetylcholine from the cell [16]; Possesses antimicrobial activity, incl. in the treatment of skin diseases [17,18]; In combination with citrus oil, it also has antibacterial and anti-inflammatory properties [19] Magnesium pidolate…”