Effects of Mad Honey on Some Biochemical Parameters in Rats

Şahin, Hüseyin; Yıldız, Oktay; Kolaylı, Sevgi

doi:10.1177/2156587215596430

Cited by 12 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In similar studies previously done, Silici et al 18,19 reported that the level of GTX-III in mad honey given to experimental animals was 6.22 μg g −1 . Sahin et al 67 reported that the level of GTX-III in the mad honey given to rats was in the means of 39.94 ± 0.02 μg g −1 . Kurtoğlu et al 87 found to be GTX-I and GTX-III as 20.4 ± 1.69 μg g −1 and 8.20 ± 1.93 μg g −1 , respectively, in order to determine the GTX levels of collected mad honey.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

et al. 2017

View full text Add to dashboard Cite

A total of 66 male Wistar rats were used and six groups (control: 10 animals and experimental: 12 animals) were formed. While a separate control group was established for each study period, mad honey application to the animals in the experimental group was carried out with a single dose (12.5 g kg body weight (b.w.); acute stage), at a dose of 7.5 g kg b.w. for 21 days (subacute stage), and at a dose of 5 g kg b.w. for 60 days (chronic stage). Tissue and blood oxidative stress markers (malondialdehyde (MDA), nitric oxide (NO), 4-hydroxynonenal (HNE), superoxide dismutase, catalase, glutathione (GSH) peroxidase, and glucose-6-phosphate dehydrogenase), hepatic chemical metabolizing parameters in the liver (cytochrome P450 2E1, nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase, nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c reductase (CYTC), GSH S-transferase (GST), and GSH), and micronucleus and comet test in some samples were examined. Findings from the study showed that single and repeated doses given over the period increased MDA, NO, and HNE levels while decreasing/increasing tissue and blood antioxidant enzyme activities. From hepatic chemical metabolizing parameters, GST activity increased in the subacute and chronic stages and CYTC activity increased in the acute period, whereas GSH level decreased in the subacute stage. Changes in tail and head intensities were found in most of the comet results. Mad honey caused oxidative stresses for each exposure period and made some significant changes on the comet test in certain periods for some samples obtained. In other words, according to the available research results obtained, careless consumption of mad honey for different medical purposes is not appropriate.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The dose used was determined by taking into account the LD50 values of GTX 65,66 as well as previous studies with mad honey. 18,19,67 All mad honey applications were made directly into stomach by gavage without using vehicle. Research protocol was received the approval of Erciyes University experimental animals Ethical Board.…”

Section: Methodsmentioning

confidence: 99%

Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The MDA, CAT, SOD, and GSH-PHx activities in the blood and tissue samples have shown negative effects on the antioxidant parameters in the groups with GTx and high doses of RH (Silici & Atayoglu ). Rats that have been applied with different doses of RH for 8 days have shown an increase in liver enzymes (Sahin et al, 2015). It has been reported that RH has a hepatoprotective effect and that the R. Arboreum species decreases cholesterol and triglycerides (Prakesh et al, 2008;Verma et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Rhododendron Honey on Mice Liver Tissue

et al. 2016

View full text Add to dashboard Cite

ILTER, G.; SÖYLER, G.; RASGELE, P. G.; KEKEÇOGLU, M & KAMBUR, M. The effect of rhododendron honey on mice liver tissue. Int. J. Morphol., 34(3): 842-847, 2016. SUMMARY: Rhododendron honey, made by bees from rhododendron pollen, contains a toxic substance called grayanotoxin. Depending on the dose, the poisonous honey can result in serious effects such as cardiac arrhythmia, fibrillation, and myocardial infarction. The purpose of this study is to investigate the effects of the poisonous RH of the Black Sea Region on the liver. Male mice were divided into five groups of twelve mice each, two being the control groups (distilled water) and the others being the rhododendron honey (RH) groups (25, 50, and 75 mg/kg) and 0.01 mg/kg grayanotoxin (GTx) groups. Liver tissues were collected 24 and 48 h later. The sections were stained with hematoxylin, eosin and PAS, then the histopathological score was performed. Significant statistical differences were observed between the RH and control groups in terms of congestion, steatosis, sinusoid dilatation, and inflammation. The control group demonstrated a normal liver structure in the light microscopy, while the GTx-applied 24 h group exhibited expansions in the sinusoids and congestion. Higher levels of congestion, steatosis, and inflammatory cells were seen in the GTx-applied 48 h group. In the same group, giant cells consisting of many nuclei were observed in the sinusoids. The results of the 25 mg RH-applied groups were similar in 24 and 48 h, histopathological score levels were increased slightly, congestion and steatosis were prominent in the 48 h group. Dense steatosis was seen in the hepatocytes around the vena centralis in 50 mg/kg RH-applied 48 h group. Congestion, steatosis and an increase in inflammatory cells were observed in the hepatocytes in the 75 mg/kg RH-applied 24-and 48 h groups. PAS (+) stained hepatocytes were decreased in the RH-and GTx-applied groups. The toxic effects of the rhododendron honey were observed in the mice liver tissue with respect to dose and time. KÜKNER, A.; ILTER, G.; SÖYLER, G.; RASGELE, P. G.; KEKEÇOGLU, M & KAMBUR, M. The effect of rhododendron honey on mice liver tissue. Int. J. Morphol., 34(3):842-847, 2016.

show abstract

“…Yine başka çalışmalarda, oral yolla gavaj şeklinde ya da i.p. olarak verilen grayanotoksin-III, kanda bazı biyokimyasal parametreleri belirgin şekilde artırmış ancak renal fonksiyonlarda bir bozulmaya neden olmamıştır (23)(24)(25)(26)(27) . Yakın zaman önce Türkmen ve ark.…”

Section: Introductionunclassified

“…Az sayıda deneysel çalışmada bal ekstraktı halinde hayvanlara oral, i.p. ve intraserebroventriküler gibi farklı yollarla uygulanmıştır (23)(24)(25)(26)(27)(28) . Çalışmamızda, deli bal içerisinde en fazla bulunan ve oldukça toksik olduğu bilinen grayanotoksin-III, daha önceki çalışmalardan farklı olarak i.v.…”

Section: Introductionunclassified

The dose-dependent cardiovascular eﬀects of intravenous grayanotoxin-III in rats

Yılmaz¹,

Kaya²,

Yaykaşlı³

et al. 2017

BUCH

View full text Add to dashboard Cite

ÖZAmaç: Deli bal zehirlenmelerin büyük bir kısmı ülkemizde görülmektedir. Deli bal içinde bulunan toksinlerin temel kaynağı Rhododendron türü bitkiler olup, bu bitkilerdeki toksinlerden en fazla grayanotoksin-III bulunur. Deli bal zehirlenmelerinde klinikte bradikardi, atriyoventriküler blok, asistoli, hipotansiyon, salivasyon gibi semptomlar görülür. Bu çalışmada, sıçanlara farklı dozlarda intravenöz (i.v.) yolla verilen grayanotoksin-III'ün doza bağımlı kardiyovasküler etkilerinin değerlendirilmesi amaçlanmıştır. Yöntem: Çalışmada, 42 Wistar albino sıçan kullanılmış ve hayvanlar Kontrol, Grup 1 (5 µg/kg), Grup 2 (10 µg/kg), Grup 3 (20 µg/kg), Grup 4 (50 µg/kg) ve Grup 5 (100 µg/kg) olmak üzere altı gruba ayrılmıştır. Elektrokardiyogram (EKG), tansiyon arteriyel ve solunum sayısı için bazal değerler elde edildikten sonra i.v. grayanotoksin uygulanmasının ardından 60 dk. boyunca bu parametreler kaydedilmiştir. Bulgular: 5 µg/kg grayanotoksin-III verilen grupta, tüm zamanlarda anlamlı bir deği-şiklik olmazken, 10 ve 20 µg/kg toksin verilen gruplarda 2. ve 5. dk.'dan sonra anlamlı biçimde solunum sayıları ve kalp tepe atım sayıları azalmış, arteryel basınçlarda düşme görülmüştür. Yirmi beşinci dk.'dan sonra ise tüm parametreler bazal değerlere yeniden geri dönmüştür. Elli ve 100 µg/kg grayanotoksin-III verilen gruplarda, 2. dk.'dan itibaren tüm parametrelerde hızla düşme olmuş ve 5-10 dk. içinde sıçanlar ölmüştür. Sonuç: Sıçanlar için i.v. grayanotoksin-III'ün 50 µg/kg ve üzerindeki yüksek dozlarda lethal olabildiği, ölümün kardiyak arrest nedeniyle görüldüğü, 5 µg/kg ve altındaki düşük dozlarda toksik etkilerin görülmediği, i.v. uygulamada toksik etkilerin yaklaşık olarak 25 dk. sürdüğü saptanmıştır. Anahtar kelimeler: Grayanotoksin-III, deli bal, kardivasküler etkiler ABSTRACTObjective: The mad honey poisonings are commonly seen in our country. The source of the toxin in mad honey is plants of Rhododendron species. Grayanotoxin-III type toxin is commonly found in these plants. Clinical effects of this toxin are bradycardia, atrioventricular block, asystole, hypotension and symptoms such as salivation. In this study; different doses of grayanotoxin-III were intravenously administered to rats (i.v) and the effect of grayanotoxin on cardiovascular system was evaluated. Methods: This study was performed on 42 Wistar albino rats and the animals were divided into six groups. Control, Group 1 (5 µg/kg), Group 2 (10 µg/kg), Group 3 (20 µg/kg), Group 4 (50 µg/kg) and Group 5 (100 µg/kg) groups were formed. Baseline values for electrocardiography (ECG), arterial blood pressure and respiratory rate were obtained and recorded for 60 minutes after administration of i.v. grayanotoxin-III. Results: Group1 (5 µg/kg) was not significantly changed in all time periods. In 10 and 20 µg/kg groups, respiratory rates, peak heart rates decreased significantly and drop in arterial blood pressures was observed after 2 nd and 5 th minutes. While 25 minutes after administration of i.v. grayanotoxin-III all parameters returned to baseline v...

show abstract

Effects of Mad Honey on Some Biochemical Parameters in Rats

Cited by 12 publications

References 14 publications

Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

The Effect of Rhododendron Honey on Mice Liver Tissue

The dose-dependent cardiovascular eﬀects of intravenous grayanotoxin-III in rats

Contact Info

Product

Resources

About