Effects of <i>inactive parapoxvirus ovis</i> on cytokine levels in rats

Abstract

The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammatory cytokine levels in rats. iPPOV (1 ml/rat) was administered intraperitoneal route to 49 rats, except for 7 rats (Control, 0 group). Serum samples were collected from 7 rats at 1st, 2nd, 4th, 8th, 12th, 16th and 24th hr after treatments. Levels of TNF-α, IL-6, IL-12 and IL-10 were determined using ELISA. Administration of iPPOV stimulated TNF-α (16th and 24th hr) and IL-6 (12th, 16th and 24th hr) synthesis and caused fluctuations in IL-10 and IL-12 concentrations. In conclusion, increased cytokine levels could be attributed to immunomodulatory activity of iPPOV, however, detailed studies are required to fully understand effects of iPPOV on immune system.

Role of Interferon (IFN)α in “Cocktails” for the Generation of (Leukemia-derived) Dendritic Cells (DCleu) From Blasts in Blood From Patients (pts) With Acute Myeloid Leukemia (AML) and the Induction of Antileukemic Reactions

“…We could already show, that the addition of Zylexis increased proportions of mature DC 47 . Therefore, we studied the role of Zylexis (a pox virusproduct, known in veterinarian medicine to improve immunological reactions, especially of the innate immune system [93][94][95] in case of DC-generation with "IFN-GIT" and additionally added Zylexis "IFN-GITZ". We found, that IFNα-containing "IFN-GIT" and "IFN-GITZ" DC-generating methods induced comparable amounts of DC-subtypes.…”

Investigación de parámetros bioquímicos y perfil de citocinas en ovejas con ectima contagioso

“…Se produjo una respuesta compleja de citocina en la que la respuesta de citocina Th1 fue seguida por la respuesta Th2 (27). Avcı et al (28) administraron iPPOV a ratas por vía intraperitoneal para investigar sus efectos sobre los niveles de citocina antiinflamatoria y proinflamatoria. Se tomaron muestras de suero a intervalos horarios para TNF-α (16 y 24 horas) e IL-6 (12, 16 y 24 horas).…”

“…Las síntesis se estimularon provocando ondulaciones en los niveles de IL-10 e IL-12. Los niveles aumentados de citocina fueron asociados con la actividad inmunomoduladora del iPPOV (28). Wang et al (29) estudiaron la respuesta a las células objetivo después de la infección por el virus orf en las células de fibroblasto de la piel de cabra, que es el objetivo principal de la receptora.…”