Effects of &lt;i&gt;Candida norvegensis&lt;/i&gt; Live Cells on &lt;i&gt;In vitro&lt;/i&gt; Oat Straw Rumen Fermentation

Ruiz, O.; Castillo-Castillo, Yamicela; Arzola, C.; Burrola, Eduviges; Salinas, J.; CORRAL, Agustín; Hume, Michael E.; Murillo, Manuel; Itza, Mateo

doi:10.5713/ajas.15.0166

Cited by 16 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to this, Levazot 15 is considered the most promising yeast strain to be used as a microbial additive to improve digestive fermentation of ruminants fed fibrous diets. The higher gas production level obtained with this strain is probably due to an increase in propionic acid synthesis, 35 which facilitates the formation of carbon dioxide (CO 2 ) via the succinate–propionate metabolic pathway. 36 In vivo and in vitro studies have shown increased rates of cellulose and NDF degradation in the presence of yeasts, 37 , 38 which could explain the increase in the gas production level found in this work.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of yeasts from fermented apple bagasse as additives for ruminant feeding

Castillo-Castillo¹,

Ruiz-Barrera

Burrola-Barraza

et al. 2016

Brazilian Journal of Microbiology

View full text Add to dashboard Cite

Solid-state fermentation can be used to produce feeds for ruminants, which can provide an enriched population of yeasts to improve ruminal fermentation. Fermentation of apple bagasse was performed to obtain a yeast-rich product, with the objective of isolating, identifying, and characterizing yeast strains and testing their capability to enhance in vitro ruminal fermentation of fibrous feeds. Yeasts were isolated from apple bagasse fermented under in vitro conditions, using rumen liquor obtained from cannulated cows and alfalfa as a fibrous substrate. A total of 16 new yeast strains were isolated and identified by biochemical and molecular methods. The strains were designated Levazot, followed by the isolate number. Their fermentative capacity was assessed using an in vitro gas production method. Strain Levazot 15 (Candida norvegensis) showed the greatest increase in gas production (p < 0.05) compared with the yeast-free control and positively affected in vitro ruminal fermentation parameters of alfalfa and oat straw. Based on these results, it was concluded that the Levazot 15 yeast strain could be potentially used as an additive for ruminants consuming high-fiber diets. However, further studies of effects of these additives on rumen digestion, metabolism, and productive performance of ruminants are required.

show abstract

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of yeasts from fermented apple bagasse as additives for ruminant feeding

Castillo-Castillo¹,

Ruiz-Barrera

Burrola-Barraza

et al. 2016

Brazilian Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…Based on these results, the Levazoot 15 yeast strain could be potentially used as an additive for ruminants consuming high-fiber diets. In another study, Ruiz et al [38] showed that the same strain of yeast did not affect the ruminal cellulolytic bacterial counts. However, the treatment with yeast cultures positively influenced the cellulolytic fungal populations in rumen after 4 h of incubation.…”

Section: Other Non-saccharomyces Strains Of Yeasts With Potential Commentioning

confidence: 95%

Yeasts as Dietary Additives to Manipulate Ruminal Fermentation: Effect on Nutrient Utilization and Productive Performance of Ruminants

Ruiz-Barrera¹,

Salinas-Chavira²,

Castillo-Castillo³

2019

Yeasts in Biotechnology

View full text Add to dashboard Cite

There is considerable interest in the use of microbial additives such as yeasts in the nutrition of ruminants. The prohibition of the antibiotics as growth promoters in animal feeds increased the interest to investigate the effects of yeasts as natural additives on the gastrointestinal ecosystem and animal productive behavior. The effect of yeast-based preparations on the rumen environment and on the growth performance of ruminants has been well documented and has generated considerable scientific attention in the last two decades. However, the precise action modes by which the yeast cultures improve nutrient utilization and livestock production are still under study. Therefore, the objective of this chapter is to deepen into the action mechanisms of the yeasts at the ruminal level and at the productive level for their use as additives in animal feeding.

show abstract

“…We performed two experimental designs based on in vitro ruminal fermentation tests. Regarding the debate between in vitro and in vivo tests, it is worth highlighting that there is a consensus of several experts (Yáñez- Ruiz et al, 2016;Danielsson et al, 2017) in that IVtFT is very useful for screening diets and preliminary evaluation of feed additives and inhibitory agents of CH 4 production in ruminal fermentation. They also advise running IVvFT after the IVtFT to confirm the main results as well as to gain more information that can only be obtained in IVvFT, before making decisions or implementing strategies at the commercial level.…”

Section: Experimental Designmentioning

confidence: 99%

“…Instead of using Lv or other specific chemical inhibitors, a promising avenue for ruminal CH 4 mitigation is the use of cultures (for instance, yeast cultures) as an inhibitor of CH 4 production in ruminal fermentation. In the work of Ruiz et al (2016), the authors studied the effects of the supplementation of Candida norvegensis (Cn) (live cells and likely metabolites present in the used medium) on the ruminal fermentation of oat straw (as the sole source of carbon, no actual cattle ration was used). Their results hold promise for developing an original approach to mitigation of ruminal CH 4 generation.…”

Section: Effect Of Lovastatin Concentration On Total Gas Production Ch 4 Hydrogen and Short-chain Fatty Acidsmentioning

confidence: 99%

Effects of Fermented Oat Straw as a Lovastatin Carrier on in vitro Methane Production and Rumen Microbiota

et al. 2021

View full text Add to dashboard Cite

To date, there is an urgent need for implementing practical strategies to reduce CH4 emissions from ruminants. Lovastatin (Lv) is a specific inhibitor of methanogenic archaea. Due to the high cost of pure Lv, solid-state fermentation might be an economical bioprocess to produce Lv and facilitate its use in ruminant nutrition. The goal of this work was to assess the effects of supplementing fermented oat straw as a lovastatin carrier (FOS) to a high-grain ration on in vitro CH4 inhibition and rumen microbiota in beef cattle. The experimental design of in vitro rumen fermentation was completely randomized with four concentrations of Lv in the diet mixture. The supplementation with FOS to give Lv concentration of 100 and 150 mg L−1 in the ruminal fermentation medium significantly inhibited methanogenesis at similar levels. This suggested that less than 20% of FOS was required in the ration to achieve up to 38% of CH4 mitigation without affecting the chemical composition and nutritional value of the ration. Short-chain fatty acid (SCFA) production and profile showed that only the treatments with Lv at 100 and 150 mg L−1 decreased the concentration of total SCFAs; the molar ratio of propionate significantly increased with respect to that of the control. Treatment with Lv at 150 mg L−1 did not result in significant differences in the alpha and beta diversity indices compared to the control. However, significant changes in the relative abundance of some microorganisms were detected, such as an increase in Ruminococcus and a decrease in Prevotella. The predominant 99%+ MA in all controls, treatment, and inocula samples belonged to the Methanobrevibacter genus and very small (negligible) unclassified Methanobacterium genus (Euryarchaeota phylum). Interestingly, the reduction of relative abundance of MA was 39.17%, very close to the percent reduction of CH4 production, 38%. Our data showed that there was a parallel and similar percent decrease of both CH4 production and relative abundance of the predominant MA in our experiment, although the statistical significance was not complete. Finally, our results hold promise for significantly decreasing ruminal CH4 by 38%. Thus, our work is one step toward the sustainable management of the livestock sector.

show abstract

Effects of <i>Candida norvegensis</i> Live Cells on <i>In vitro</i> Oat Straw Rumen Fermentation

Cited by 16 publications

References 37 publications

Isolation and characterization of yeasts from fermented apple bagasse as additives for ruminant feeding

Isolation and characterization of yeasts from fermented apple bagasse as additives for ruminant feeding

Yeasts as Dietary Additives to Manipulate Ruminal Fermentation: Effect on Nutrient Utilization and Productive Performance of Ruminants

Effects of Fermented Oat Straw as a Lovastatin Carrier on in vitro Methane Production and Rumen Microbiota

Contact Info

Product

Resources

About