Effects of Low Temperatures on Enzyme Activity, Chlorophyll and Ion Contents in Common Bean Genotypes

Abstract

Low temperatures slow down plant growth and development and generate physiological damages on plants. Metabolism of largely produced and consumed common bean (Phaseolus vulgaris L.) plants decelerates at temperatures below 15 °C. This study was carried out at Van Yuzuncu Yil University in Van province of Turkey with limited vegetation period due to its cold climate in winter. In this study, the bean plants were subjected to low temperature stress and catalase (CAT), super oxide dismutase (SOD), ascorbate peroxidase (APX) enzyme activities, malondialdehyde (MDA), chlorophylla, chlorophyll-b and total chlorophyll, K, Ca, and Mg ion contents of low temperature stress-tolerant Yakutiye and Ç30 and low temperature stress-sensitive Zulbiye and Ç13 bean genotypes were measured on 25 th , 30 th , 35 th and 40 th days of low temperature stress. Four bean seeds were sown in 2-liter pots filled with 2:1 peat:perlite mixture as to have 2 plants in each pot. The pots were irrigated with Hoagland nutrient solution. Low temperature stress plants were sown on 15 th of March with low outdoor temperatures. In control group, been seeds were sown on 25 th of April and plant samples were taken in again 5-day intervals in 4 periods (25 th , 30 th , 35 th and 40 th days of growth). Significant differences were observed in enzyme activities, chlorophyll contents and ion contents of low temperature-tolerant and sensitive bean genotypes in 4 measurement periods.

Kuraklık Stresinin Urfa Biberinde İyon Klorofil ve Enzim İçerikleri Üzerine Etkisi

“…Bu nedenle kuraklık, tarımsal üretim için en önemli sınırlayıcı faktörler arasında yer almaktadır (Liu ve Stützel, 2004;Özfidaner vd., 2018). Abiyotik streslerde reaktif oksijen türlerinin (ROS), bitkilerde strese karşı toleransın artırılmasında önemli rol oynadığı düşünülmektedir (Kabay 2018a;Kabay 2018b). Kuraklık stresi bitkilerde enzim aktivitesi ve enzim miktarı üzerine de önemli bir etki yapar.…”

Physiological and transcriptomic analysis of tomato in response to sub-optimal temperature stress

“…Similarly, previous study also found that there were greater decrease of chlorophyll a and b contents in low temperature sensitive common bean genotype than in tolerant one. 43 This indicates that the photosynthetic system of T722 suffered minor damage. Chlorophyll fluorescence emission is a noninvasive, effective, and reliable technique for detecting photosystem II (PSII) damage in plants exposed to abiotic stress environment.…”

NO and GSH Alleviate the Inhibition of Low-Temperature Stress on Cowpea Seedlings

“…Excessive ROS content in plant cells will destroy the ROS balance mechanism [ 8 , 9 ], but they will remove harmful H 2 O 2 and O 2 − from plants through the circulation of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) enzyme systems, and glutathione–ascorbic acid (GSH–AsA) [ 10 ]. Low-temperature stress on beans will affect the yield, quality, and metabolic rate of kidney beans [ 11 ]. There are two stages of sensitivity to low temperature: the first is the seedling stage [ 12 ] and the second is the flowering stage.…”