Effects of Low-Power Laser Irradiation on Cell Locomotion in Protozoa

Koutna, Marketa; Janisch, Roman; Unucka, Marek; Svobodník, Adam; Mornstein, Vojtěch

doi:10.1562/2004-02-19-ra-087

Cited by 6 publications

(9 citation statements)

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“…The LLL interacts with different types of tissues and LLL therapy (LLLT) is known to cause varied reactions and modulate several biological processes. It has been demonstrated that the photo-stimulation mediated by the LLLT, in addition to increase ATP synthesis [6], produces an analgesic effect [7][8][9] and stimulates cell division [10], accelerating tissue healing [11][12][13]. Some specific laser types also have the capacity to stimulate the synthesis and deposition of collagen matrix by fibroblasts [14][15][16], and the increase of mitochondrial activity and cell metabolism [8] and proliferation [12].…”

Section: Introductionmentioning

confidence: 99%

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Tagliani¹,

Oliveira²,

Lins³

et al. 2010

Laser Phys. Lett.

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In spite of knowing that cells under stress are biostimulated by low level laser (LLL) irradiation, the ideal condition of stress to different cell lines has not yet been established. Consequently, the aim of the present in vitro study was to evaluate the effects of a defined parameter of LLL irradiation applied on stressed odontoblast-like pulp cells (MDPC-23). The cells were seeded (12500 cells/cm 2 ) in wells of 24-well plates using complete culture medium (DMEM) and incubated for 24 hours. Then, the DMEM was replaced by a new medium with low concentrations (nutritional stress condition) of fetal bovine serum (FBS) giving rise to the following experimental groups: G1: 2% FBS; G2: 5% FBS; and G3: 10% FBS. The cells were irradiated three times with LLL in specific parameters (808±3 nm, 100 mW, 1.5 J/cm 2 ) every 24 hours. No irradiation was carried out in groups G4 (2% FBS-Control), G5 (5% FBS-Control), and G6 (10% FBS-Control). For all groups, the cell metabolism (MTT assay) and morphology (SEM) was evaluated. The experimental groups showed enhanced cell metabolism and normal cell morphology regardless of FBS concentration. A slight increase in the cell metabolism was observed only in group G2. It was concluded that cell nutritional stress caused by reducing the concentration of FBS to 5% is the most suitable method to assess the biostimulation of LLL irradiated MDPC-23 cells.MDPC-23 cells adhered to the glass substrate exhibited a spindle-shaped morphology and some mitosis were observed (arrows); SEM ×500

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Section: Introductionmentioning

confidence: 99%

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Tagliani¹,

Oliveira²,

Lins³

et al. 2010

Laser Phys. Lett.

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“…Several studies have demonstrated that biostimulation by low-level laser therapy (LLLT) [1][2][3][4] in addition to increase ATP synthesis [5] also produces an analgesic effect [6], acceleration of tissue healing [7,8], triggering of acid nucleic production and increase of cell division, proliferation and metabolism [8][9][10]. Some specific types of laser have also the capacity to stimulate the synthesis and deposition of collagen matrix by fibroblasts [11][12][13] due to the primary action of infrared light on lysosomes and mitochondria [14].…”

Section: Introductionmentioning

confidence: 99%

Effect of low-level laser irradiation on odontoblast-like cells

et al. 2008

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Low-level laser therapy (LLLT), also referred to as therapeutic laser, has been recommended for a wide array of clinical procedures, among which the treatment of dentinal hypersensitivity. However, the mechanism that guides this process remains unknown. Therefore, the objective of this study was to evaluate in vitro the effects of LLL irradiation on cell metabolism (MTT assay), alkaline phosphatase (ALP) expression and total protein synthesis. The expression of genes that encode for collagen type-1 (Col-1) and fibronectin (FN) was analyzed by RT-PCR. For such purposes, odontoblast-like cell line (MDPC-23) was previously cultured in Petri dishes (15000 cells/cm 2 ) and submitted to stress conditions during 12 h. Thereafter, 6 applications with a monochromatic near infrared radiation (GaAlAs) set at predetermined parameters were performed at 12-h intervals. Nonirradiated cells served as a control group. Neither the MTT values nor the total protein levels of the irradiated group differed significantly from those of the control group (Mann-Whitney test; p > 0.05). On the other hand, the irradiated cells showed a decrease in ALP activity (Mann-Whitney test; p < 0.05). RT-PCR results demonstrated a trend to a specific reduction in gene expression after cell irradiation, though not significant statistically (Mann-Whitney test; p > 0.05). It may be concluded that, under the tested conditions, the LLLT parameters used in the present study did not influence cell metabolism, but reduced slightly the expression of some specific proteins.

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“…Maxillary expansion causes hyperemia, osteoblastic activity, primary bone regeneration, and calcium removal. [ 6 7 8 9 ]…”

Section: Introductionmentioning

confidence: 99%

“…Since 1971, studies have evaluated the effect of laser on bone regeneration in clinical conditions or cell cultures. [ 2 4 5 9 10 11 12 13 14 15 ] Lasers can be used in biopsy sampling, eliminating oral lesions (aphthous ulcers, herpetic lesions), tuberosity reduction, coagulation, treating temporomandibular joint lesions, instrument sterilization, gingivectomies, etc. [ 2 4 5 7 8 9 10 11 12 13 14 15 16 17 18 19 ]…”

Section: Introductionmentioning

confidence: 99%

“…Regeneration is a complicated process and laser might accelerate it. [ 2 4 5 8 9 10 11 12 13 14 20 21 22 ] In a study, Saito and Shimizu investigated the effect of gallium-aluminum-arsenide (Ga-Al-As) low-power laser exposure on bone regeneration in palatal midsuture during the maxillary expansion of rats. [ 4 ] Results of histophotometric investigations showed that compared with the control group, bone regeneration increased approximately 1.2–1.4 times in 7 days in the experimental group.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the effect of laser irradiation on bone regeneration in midpalatal suture concurrent to rapid palatal expansion in rats

Amini

Abadi²,

Mollaei

2015

J Orthodont Sci

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Background:Rapid palatal expansion is one of the most important orthopedic treatments that correct the dental and palatal constriction. Stability of the changes partly depend on the rapidity of new bone formation in affected sutures after expansion. The purpose of this study was to investigate the effect of laser irradiation on the healing of midpalatal suture concurrent to the expansion of midpalatal suture in rats.Materials and Methods:A total of 78 male Sprague rats in seven groups were evaluated: A control group of six rats without any treatments and three experimental groups of 24 which underwent palatal expansion for different time periods (7, 14, and 30 days), and each divided into two groups of with and without laser irradiation. Laser therapy was done by gallium-aluminum-arsenide diode laser with 810 nm wavelength and 4 J/cm2 irradiation in days 0, 2, 4, 6, 8, 10, 12, 14 in 4 points (1 labial and 3 palatal points). After sacrificing, the sections were evaluated by histomorphometric and quantitative analysis and results were statistically investigated by independent samples t-test.Results:The results in 7 days, 14 days, and 30 days show that laser therapy can increase the rate of osteogenesis in palatal suture during rapid palatal expansion but the differences in 7 days groups were not significant (P = 0.117) while in 14 days groups (P = 0.032) and 30 days groups were significant (P = 0.001). Most of effectiveness of low-power laser was seen between 14 and 30 days while the laser therapy was stopped.Conclusion:These findings suggest that low-level laser irradiation can increase and accelerate bone regeneration in the midpalatal suture after rapid palatal expansion, hence, reduce retention time.

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Effects of Low-Power Laser Irradiation on Cell Locomotion in Protozoa

Cited by 6 publications

References 0 publications

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Effect of low-level laser irradiation on odontoblast-like cells

Evaluating the effect of laser irradiation on bone regeneration in midpalatal suture concurrent to rapid palatal expansion in rats

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