Effects of Low pH on the Induction of Root Hair Formation in Young Lettuce (Lactuca sativa L. cv. Grand Rapids) Seedlings

Inoue, Yasunori; Yamaoka, Keiko; Kimura, Kyoko; Sawai, Kaori; Arai, Takashi

doi:10.1007/pl00013909

Cited by 32 publications

(10 citation statements)

References 28 publications

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“…Based on this report, we suggest that the decrease in Mn uptake at low pH is caused not by decreased Mn availability in the medium, but by a decrease in Mn uptake by lettuce roots. Inoue et al (2000) reported that root elongation was suppressed at low pH but this condition had little effect on the fresh weight of the shoot. Therefore, we considered the possibility that shoot growth could be suppressed by the excess Mn at pH 4, because of the suppression of root hair induction at low pH.…”

Section: Discussionmentioning

confidence: 96%

Role of manganese in low-pH-induced root hair formation in Lactuca sativa cv. Grand Rapids seedlings

Konno

Ooishi

Inoue

2003

Journal of Plant Research

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Root hair formation is induced by low pH in lettuce ( Lactuca sativa L. cv. Grand Rapids) seedlings cultured in mineral medium. The role of mineral concentrations in this phenomenon was investigated, especially for manganese. When lettuce seedlings were cultured in media that were deficient in calcium (Ca), manganese (Mn), boron (B) or molybdenum (Mo), morphological changes were induced in roots. Deficiency of other nutrients had little effect on root hair formation. Ca or B deficiency inhibited the growth of the main root and the formation of root hairs, regardless of pH. Mn or Mo deficiency increased root hair formation at pH 6 and suppressed main root growth slightly. In contrast, increasing the Mn concentration suppressed low-pH-induced root hair formation. The Mn content of roots grown at pH 4 was only about 15% of that at pH 6. In contrast, the Mo content of roots grown at low pH was about six times that of roots grown at neutral pH. These results suggest that root hair formation induced by low pH is at least partly mediated by decreased Mn uptake in root cells.

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Section: Discussionmentioning

confidence: 96%

Role of manganese in low-pH-induced root hair formation in Lactuca sativa cv. Grand Rapids seedlings

Konno

Ooishi

Inoue

2003

Journal of Plant Research

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“…It is a well-known phenomenon that acidification enhances activity of expansins (McQueenMason et al, 1992). Decreasing pH to a value of 4.0 to 4.5 strongly stimulated growth of root hairs in Lactuca sativa (Inoue et al, 2000). Accumulation of expansins in the cell wall domain in place of subsequent root hair outgrowth can promote local disassembling of cell wall components.…”

Section: Discussion the Role Of Expansins In Root Hair Developmentmentioning

confidence: 99%

Molecular Cloning and Characterization of β-Expansin Gene Related to Root Hair Formation in Barley

2006

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Root hairs are specialized epidermal cells that play a role in the uptake of water and nutrients from the rhizosphere and serve as a site of interaction with soil microorganisms. The process of root hair formation is well characterized in Arabidopsis (Arabidopsis thaliana); however, there is a very little information about the genetic and molecular basis of root hair development in monocots. Here, we report on isolation and cloning of the b-expansin (EXPB) gene HvEXPB1, tightly related to root hair initiation in barley (Hordeum vulgare). Using root transcriptome differentiation in the wild-type/root-hairless mutant system, a cDNA fragment present in roots of wild-type plants only was identified. After cloning of full-length cDNA and genomic sequences flanking the identified fragment, the subsequent bioinformatics analyses revealed homology of the protein coded by the identified gene to the EXPB family. Reverse transcription-PCR showed that expression of HvEXPB1 cosegregated with the root hair phenotype in F 2 progeny of the cross between the hairless mutant rhl1.a and the wild-type Karat parent variety. Expression of the HvEXPB1 gene was root specific; it was expressed in roots of wild-type forms, but not in coleoptiles, leaves, tillers, and spikes. The identified gene was active in roots of two other analyzed root hair mutants: rhp1.a developing root hair primordia only and rhs1.a with very short root hairs. Contrary to this, a complete lack of HvEXPB1 expression was observed in roots of the spontaneous root-hairless mutant bald root barley. All these observations suggest a role of the HvEXPB1 gene in the process of root hair formation in barley.

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“…Bethke et al 23 demonstrated the existence of apoplastic synthesis of NO from nitrite that requires low pH. Inoue et al 24 demonstrated that low pH induces RHF in young lettuce seedlings. Interestingly, it was clearly demonstrated that low pH (~4.5) at cell wall bulging of trichoblasts is required at the initiation phase of RHF.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Functions as a Positive Regulator of Root Hair Development

Lombardo

Graziano²,

Polacco

et al. 2006

Plant Signaling & Behavior

244

188

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KEY WORDS Research PaperNitric Oxide Functions as a Positive Regulator of Root Hair Development ABSTRACTThe root epidermis is composed of two cell types: trichoblasts (or hair cells) and atrichoblasts (or non-hair cells). In lettuce (Lactuca sativa cv. Grand Rapids var. Rapidmor oscura) plants grown hydroponically in water, the root epidermis did not form root hairs. The addition of 10 µM sodium nitroprusside (SNP), a nitric oxide (NO) donor, resulted in almost all rhizodermal cells differentiated into root hairs. Treatment with the synthetic auxin 1-naphthyl acetic acid (NAA) displayed a significant increase of root hair formation (RHF) that was prevented by the specific NO scavenger carboxy-PTIO (cPTIO). In Arabidopsis, two mutants have been shown to be defective in NO production and to display altered phenotypes in which NO is implicated. Arabidopsis nos1 has a mutation in an NO synthase structural gene (NOS1), and the nia1 nia2 double mutant is null for nitrate reductase (NR) activity. We observed that both mutants were affected in their capacity of developing root hairs. Root hair elongation was significantly reduced in nos1 and nia1 nia2 mutants as well as in cPTIO-treated wild type plants. A correlation was found between endogenous NO level in roots detected by the fluorescent probe DAF-FM DA and RHF. In Arabidopsis, as well as in lettuce, cPTIO blocked the NAA-induced root hair elongation. Taken together, these results indicate that: (1) NO is a critical molecule in the process leading to RHF and (2) NO is involved in the auxin-signaling cascade leading to RHF.

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Effects of Low pH on the Induction of Root Hair Formation in Young Lettuce (Lactuca sativa L. cv. Grand Rapids) Seedlings

Cited by 32 publications

References 28 publications

Role of manganese in low-pH-induced root hair formation in Lactuca sativa cv. Grand Rapids seedlings

Role of manganese in low-pH-induced root hair formation in Lactuca sativa cv. Grand Rapids seedlings

Molecular Cloning and Characterization of β-Expansin Gene Related to Root Hair Formation in Barley

Nitric Oxide Functions as a Positive Regulator of Root Hair Development

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