Effects of Low Molecular Weight Chondroitin Sulfate on Type II Collagen-Induced Arthritis in DBA/1J Mice

Cho, So Yean; Sim, Ji Hyun; Jeong, Choon Sik; Chang, Seung Yeup; Choi, Donghyun; Toida, Toshihiko; Kim, Yeong Shik

doi:10.1248/bpb.27.47

Cited by 56 publications

(32 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Comparable results were observed in the same animal model by oral administration of low molecular weight CS (Cho et al 2004), orally administered to DBA/1J mice once daily for 14 days prior to initial immunization with type II collagen. Low molecular weight CS inhibited elastase activity slightly, but hind paw oedema was significantly diminished.…”

Section: Cs Anti-inflammatory Activity In Animal Modelsmentioning

confidence: 56%

Anti-inflammatory activity of chondroitin sulphate: new functions from an old natural macromolecule

Volpi

2011

Inflammopharmacol

102

View full text Add to dashboard Cite

Chondroitin sulphate (CS) is recommended by the European League Against Rheumatism as a symptomatic slow-acting drug for the treatment of osteoarthritis on the basis of numerous clinical trials and meta-analyses. Furthermore, recent clinical trials have also demonstrated the possible structure-modifying effects of CS. This review focuses on recent experimental results and data available in the scientific literature regarding the anti-inflammatory properties of CS with a view to understanding the molecular basis responsible for its activity. Several animal studies have demonstrated that orally administered CS significantly inhibited hind paw oedema, synovitis and destruction of the articular cartilage in a dose-dependent manner. Furthermore, CS proved to have a beneficial effect in slowing down the development of adjuvant arthritis and in reducing disease markers, findings which support its beneficial activity in humans as a chondroprotective drug. Finally, several in vitro studies have focused on the hypothesis that CS may reduce inflammatory processes by acting on the nuclear translocation of NF-κB, which is closely associated with the blood biomarkers of inflammation, primarily IL-1, IL-6 and C-reactive protein.

show abstract

Section: Cs Anti-inflammatory Activity In Animal Modelsmentioning

confidence: 56%

Anti-inflammatory activity of chondroitin sulphate: new functions from an old natural macromolecule

Volpi

2011

Inflammopharmacol

102

View full text Add to dashboard Cite

show abstract

“…CS was shown to reduce synovitis in collagen-induced arthritis in mouse [Omata et al 2000]. It was also able to reduce the pro-inflammatory cytokine, interleukin (IL)-6, in the same model [Cho et al 2004]. CS also reduced IL-1b in joint tissue in Freund's adjuvant arthritis when administered as a dietary supplement [Chou et al 2005] and TNF-a and myeloperoxidase in the plasma of rat with collagen-induced arthritis and after oral administration [Campo et al 2003].…”

Section: Mechanism Of Actionmentioning

confidence: 99%

Chondroitin sulfate in the treatment of osteoarthritis: from in vitro studies to clinical recommendations

Henrotin

Mathy

Sanchez

et al. 2010

Therapeutic Advances in Musculoskeletal

144

View full text Add to dashboard Cite

Chondroitin sulfate (CS) is recommended as a therapeutic intervention in the multimodal approach of osteoarthritis (OA) management. CS has been studied extensively to describe its pharmacology (pharmacokinetic, in vitro and in vivo effects) and its clinical efficacy. Various results have been reported depending on the system of evaluation (model, dosage and duration) and the source of CS (origin and quality). The purpose of this review was to gather most of the available information about CS and to discuss its potency in OA management.

show abstract

“…To determine the average MW of LMWAS, highperformance size exclusion chromatography (HPSEC) analysis was carried out on a TSK-G3000SW column (7.5×300 mm) and its guard column (7.5×75 mm) (Tosoh, Japan) as previously described [24,25] with minor modifications. In the case of intact AS having high molecular weight, a TSK-G6000 PWXL column (7.5× 300 mm) was used with different MW standard samples.…”

Section: Agarose Gel Electrophoresismentioning

confidence: 99%

Inhibition of Helicobacter pylori adhesion to Kato III cells by intact and low molecular weight acharan sulfate

Sim

Hahn

et al. 2011

Glycoconj J

Self Cite

View full text Add to dashboard Cite

We investigated the inhibitory activity of glycosaminoglycans (GAGs) in terms of growth, adhesion, and VacA vacuolation of Helicobacter pylori. Intact acharan sulfate (AS, MW:114 kDa) potently inhibited H. pylori adhesion to Kato III cells with IC(50) value of 1.4 mg/mL, while other GAGs did not show any inhibitory activity except for heparin which is a well-known inhibitor of H. pylori adhesion. To investigate whether low molecular weight acharan sulfate (LMWAS) can inhibit H. pylori adhesion, we performed chemical depolymerization of AS by radical reactions to obtain LMWAS. Its physicochemical properties were characterized by high-performance size exclusion chromatography (HPSEC), agarose gel electrophoresis, disaccharide compositional analysis after digestion with heparinase II, and (1)H-NMR spectroscopy. The most potent molecular size of LMWAS was 3 kDa with IC(50) value of 32 μg/mL, which is 44-fold more potent than intact AS. These results suggest that AS as well as other GAGs can be chemically depolymerized by free radicals and LMWAS compared to intact AS can be applied as a pharmaceutical candidate in order to inhibit H. pylori adhesion to Kato III cells.

show abstract

Effects of Low Molecular Weight Chondroitin Sulfate on Type II Collagen-Induced Arthritis in DBA/1J Mice

Cited by 56 publications

References 22 publications

Anti-inflammatory activity of chondroitin sulphate: new functions from an old natural macromolecule

Anti-inflammatory activity of chondroitin sulphate: new functions from an old natural macromolecule

Chondroitin sulfate in the treatment of osteoarthritis: from in vitro studies to clinical recommendations

Inhibition of Helicobacter pylori adhesion to Kato III cells by intact and low molecular weight acharan sulfate

Contact Info

Product

Resources

About