Effects of Low-Level Laser Therapy, 660 nm, in Experimental Septic Arthritis

Abstract

The effectiveness of low-level laser therapy (LLLT) in the presence of an infectious process has not been well elucidated. The aim of the study was to evaluate the effects of LLLT in an experimental model of septic arthritis. Methods. Twenty-one Wistar rats were divided as follows: control group, no bacteria; placebo group, bacteria were inoculated; Treated group, bacteria were injected and treatment with LLLTwas performed. To assess nociception, a von Frey digital analgesimeter was applied. Synovial fluid was streaked to analyze bacterial growth. The standard strain of S. aureus was inoculated in the right knee. LLLT was performed with 660 nm, 2 J/cm2, over 10 days. After treatment, the knees were fixed and processed for morphological analysis by light microscopy. Results. It was found that nociception increases in the right knee. There was a lack of results for the seeding of the synovial fluid. The morphological analysis showed slight recovery areas in the articular cartilage and synovia; however, there was the maintenance of the inflammatory infiltrate. Conclusion. The parameters used were not effective in the nociception reduction, even with the slight tissue recovery due to the maintenance of inflammatory infiltrate, but produced no change in the natural history of resolution of the infectious process.

Effect of Low-Level Laser Therapy on Bacterial Counts of Contaminated Traumatic Wounds in Dogs

“…38 On the contrary, Araujo et al did not observe any antibacterial effect on a model of septic arthritis in rats, but it should be noted that although a similar dose was used (2 J/cm 2 ), the intraarticular penetration of the 660-nm wavelength that was used was very limited. 59 Vasheghani et al did not observe an antimicrobial effect on third-degree burns in rats either, but in their case, a much lower dose (0.396 J/cm 2 ) was used. 60 The use of doses above 10 J/cm 2 for wounds remains controversial.…”

Blue and red light photoemitters as approach to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth

“…In vitro studies suggest that light at wavelengths between 625-1000 nm (from red to infrared) when applied alone does not induce increased growth of bacteria such as S. aureus and P. aeruginosa (Lipovsky et al, 2010;Lee et al, 2011;Baffoni et al, 2012;Costa and Assis, 2012;Araujo et al, 2013;Kim et al, 2013;Pereira et al, 2014). This suggests that such wavelengths do not interact with chromophores known to exist in these infectious agents.…”

Clinical and Radiographic Alterations in Bilateral Avascular Necrosis of the Femoral Head Following Laser Acupuncture: A Case Report

“…3 Various treatments have been suggested for this pathologic condition including total hip arthroplasty, cell therapy with mesenchymal stromal cells, 4 trans-trochanteric rotational osteotomy, 5 using novel calcium phosphate (CPC) composite scaffold, which contains BMP-VEGFloaded poly-lactic-co-glycolic acid microspheres, 6 core decompression and autologous bone marrow concentrate, 3 and tensor fascia lata muscle pedicle grafting. 7 Some evidence shows that low-level laser irradiation can improve wound healing 8,9 and pain relief in patients with temporomandibular joint disorders, 10 and may successfully be used in treatment of some pathological conditions like Achilles tendon injury in animal models, 11 cartilage defects, 12 experimental septic arthritis, 13 inflammatory and rheumatic diseases, 14 and other musculoskeletal lesions. Some clinical randomized control trials report the effectiveness of LLLT in musculoskeletal pains.…”