Effects of low-intensity pulsed ultrasound on the differentiation of C2C12 cells

Ikeda, Kyoko; Takayama, Tadahiro; Suzuki, Naoto; Shimada, Koji; Otsuka, Kichibee; Ito, Koichi

doi:10.1016/j.lfs.2006.06.029

Cited by 101 publications

(68 citation statements)

References 46 publications

(51 reference statements)

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“…They also demonstrated that LIPUS stimulation converts the differentiation pathway of C2C12 cells into the osteoblast and/or chondroblast lineage via activated phosphorylation of ERK1/2 and p38 MAPK. [43] Mukai et al (2005) in their in vitro study demonstrated that LIPUS promoted the mRNA expression of type II collagen, type X collagen, aggrecan, and TGF-β in rat chondrocytes. [44] In addition, Chen et al (2003) reported that LIPUS stimulation elevated Runx2 mRNA expression and gradually promoted OC mRNA expression in human osteoblasts.…”

Section: Lipus In Cellular Levelsmentioning

confidence: 99%

Untitled

Rashidi

2017

AJP

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Ultrasound (US) waves due to their unique features can be a treatment option for osteoporosis. Initial studies have shown promising but controversial outcomes. Several preclinical and clinical studies have been conducted on osteoporosis and studies are ongoing to find optimum US parameters, mechanisms of action, and therapeutic efficacies of these techniques for osteoporosis treatment. This paper was aimed to review the recent advances of using US waves in the treatment of osteoporosis and possible mechanisms of actions. The databases of PubMed (1980PubMed ( -2016, EMBASE (1980EMBASE ( -2016, Web of Sciences (1980-2016), and Google Scholar (1980-2016 were searched using the set terms. The obtained records were reviewed, and relevant studies were selected for comprehensive review of the current literature. Low-intensity pulsed US (LIPUS) has biological effects on the bone healing process and it can accelerate bone regeneration. Current evidence is limited on the efficacy of US waves for treatment or prevention of osteoporosis; however, the initial studies are promising. The US waves can promote osteoblast and inhibit osteoclast, enhance angiogenesis, trigger expression of different genes associated with osteogenesis. No definite dose-response existed on the clinical trials of US wave applications. The current evidence shows the therapeutic efficacy of US waves particularly LIPUS for osteoporosis treatment; however, to observe therapeutic outcomes long-term US stimulation is required. No definitive dose-response is proposed for osteoporosis. Further in vitro and clinical trials should be conducted to develop US-based techniques for the treatment of osteoporosis as a clinical treatment option.

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Section: Lipus In Cellular Levelsmentioning

confidence: 99%

Untitled

Rashidi

2017

AJP

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“…Another cell type that has been reported to be stimulated by LIPUS to enhance matrix production is macrophages. It has been shown that LIPUS up-regulated the phagocytosis of human primary macrophages through the activation of Src and ERKs and promoted the protein expression of CD147 and MMPs, as well as increased the level of protein tyrosine phosphorylation (Ikeda et al, 2006;Li et al, 2007a). Also, it has been reported that the effect of LIPUS on macrophages depends on cell adhesion, and relates to the integrin-MEK-ERK pathway (Li et al, 2007b).…”

Section: Effect Of Lipus On In-vitro Matrix Formation For Tissue Engimentioning

confidence: 99%

Low Intensity Pulsed Ultrasound: A Laboratory and Clinical Promoter in Tissue Engineering Abstract

El‐Bialy¹

2010

Tissue Engineering

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This chapter will focus on the current and future applications of low intensity pulsed ultrasound (LIPUS) in tissue engineering at laboratory and clinical levels. Also, this chapter will explain the molecular basis of LIPUS stimulatory effects on cell expansion, differentiation and matrix production of different tissues. In addition, this chapter will provide information about the differences between LIPUS application in the laboratory settings and in the clinical applications with emphasis on tissue engineering applications. Moreover, this chapter will discuss why variability in outcome might occur when applying LIPUS in laboratory or clinical settings. Also, we will provide some tips on how to prevent potential inconsistency in treatment results with emphasis on the technique sensitivity and how a scientist or a clinician should be aware of this sensitivity of the application technique in order to optimize the utilization of LIPUS in different settings. This chapter will set a few hypotheses and potential new applications of utilizing LIPUS in other tissue engineering applications. The outcome of this chapter will be an understanding of the advantages and limitations of using LIPUS in tissue engineering applications either at the laboratory or in clinical settings.

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“…Of course, these stages overlap and cannot be distinguished separately (5). External factors such as elasticity of the bone at the fracture site affect bone healing and stimulate bone formation, accelerate cell growth, differentiation, chemotaxis, and synthesis of extracellular matrix (7). Copyright External chemical agents such as steroids, nonsteroidal anti-inflammatory drugs (NSAIDs), and chemotherapy also affect the process of bone repairing (8,9).…”

Section: Introductionmentioning

confidence: 99%

Effects of Indomethacin and Meloxicam, Nonsteroidal Anti-inflammatory Drugs, on Tibia Fracture Union in Rats

Pahlavanhosseini¹,

Firoozabadi²,

Hassanpour³

et al. 2016

J Orthop Spine Trauma

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Background: One of the most important factors in the fracture healing is the intracellular production of prostaglandins by osteoblast cells. Nonsteroidal anti-inflammatory drugs (NSAIDs) exert their effects through inhibition of prostaglandin synthesis. NSAIDs are widely used in orthopedic practices and their effect on bone healing is not fully understood yet. Objectives: The current study aimed at examining the effects of indomethacin and meloxicam on tibia fracture union in rats. Methods: The current study was conducted on 60 male rats. Mid-shaft tibia fracture was induced in rats using bone-breaker device. The animals were randomly divided into 3 groups; a control group that received distilled water and 2 other groups that received indomethacin and meloxicam respectively for 28 days. At the end of weeks 1, 2, 4, 8 and 12, four rats were randomly sacrificed from each group, and histological evaluation, measurement of the calcium, phosphorus, and alkaline phosphatase plasma levels, as well as radiographic examinations were performed on them. Results: After 4 weeks, a thin layer of woven bone was observed in the vast spaces of the bone marrow in the fracture area in the control group. In the meloxicam group in the week 4, the formation of immature blades of bone was observed, which were less organized and more irregular. In the indomethacin group in the week 4, new bone formation was less immature and more areas of cartilage were still observed. In the radiographic evaluations, delayed union in indomethacin and meloxicam groups was observed, which was more significant in the indomethacin group. Conclusions: Indomethacin and meloxicam had impact on the process of bone repair and delayed union in both groups of drugs. This delayed union was more significant in non-selective NSAIDs (COX-I and = II inhibitors) rather than selective NSAIDs (COX-II inhibitor).

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Effects of low-intensity pulsed ultrasound on the differentiation of C2C12 cells

Cited by 101 publications

References 46 publications

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Low Intensity Pulsed Ultrasound: A Laboratory and Clinical Promoter in Tissue Engineering Abstract

Effects of Indomethacin and Meloxicam, Nonsteroidal Anti-inflammatory Drugs, on Tibia Fracture Union in Rats

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